Plant Protoplasts

Plant Protoplasts

International Review of Cytology, Vol. 16

1st Edition - January 1, 1983

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  • Editor: Kenneth L. Giles
  • eBook ISBN: 9781483218397

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Plant Protoplasts covers the techniques involved with, and uses of, protoplast technology. The book discusses isolation, fusion, and culture of higher plant protoplasts, lower plant protoplasts, and blue-green algal protoplast. The text also describes the production of haploid protoplasts from developing pollen grains; the use of protoplasts in mutant selection schemes, and the development of protoplast systems for use with monocotyledonous plants. The book will be invaluable to plant technologists, botanists, biochemists, research workers as well as advanced students interested in gaining a background knowledge of the field.

Table of Contents

  • Contributors


    Supplements in Series

    The Enzymatic Isolation of Plant Protoplasts: Historical Perspective



    Protoplasts and Spheroplasts of Cyanobacteria

    I. Introduction

    II. Cyanobacteria Wall Structures

    III. Protoplast and Spheroplast Methods

    IV. Conclusions


    Protoplasts of Nonvascular Plants

    I. Introduction

    II. Eukaryotic Algae Update

    III. Liverworts

    IV. Mosses

    V. Ferns

    VI. Conclusions


    Plant Protoplast Isolation and Culture

    I. Introduction

    II. Critical Variables for Successful Protoplast Isolation and Culture

    III. Results of Successful Protoplast Culture

    IV. Concluding Remarks and Recommendations


    Wall Regeneration around Isolated Protoplasts

    I. Introduction

    II. Criteria of Wall Formation

    III. Time Course of Wall Formation

    IV. Structural Aspects of Wall Formation

    V. Chemical Studies of Wall Formation by Protoplasts

    VI. Inhibitors of Cell Wall Regeneration

    VII. General Conclusions and Prospects


    Isolation and Culture of Protoplasts of Grasses

    I. Introduction

    II. Isolation of Protoplasts

    III. Culture of Protoplasts

    IV. Conclusions


    Protoplasts in Plant Virus Research

    I. Introduction

    II. Source of Protoplasts

    III. Infection of Protoplasts

    IV. Studies of Virus Replication Using Protoplasts

    V. Concluding Remarks


    Haploid Protoplasts

    I. Introduction

    II. Isolation and Culture of Pollen Protoplasts

    III. Isolation and Culture of Pollen-Tetrad and Pollen-Mother Cell Protoplasts

    IV. Isolation of Protoplasts from Mesophyll Cells and Callus Cell Suspension

    V. Culture of Haploid Protoplasts and the Regeneration of Plants

    VI. Fusion of Haploid Protoplasts

    VII. Genetic Variability in the Regenerated Plants

    VIII. Haploid Protoplasts and Mutations

    IX. Conclusions and Prospects


    Somatic Hybridization: Fusion Methods, Recovery of Hybrids, and Genetic Analysis

    I. Introduction

    II. Methods of Protoplast Fusion

    III. Selection of Hybrid Cells

    IV. Recovery and Classification of Recent Somatic Hybrid Plants: Research Directions

    V. Genetic Analysis

    VI. Concluding Remarks


    Protoplasts in Mutant Selection and Characterization


    II. Mutants Selected in Protoplast Culture

    III. Characterization of Mutants by Cell Fusion

    IV. Cell Fusion to Introduce New Mutations into Functional Plants

    V. Concluding Remarks


    Protoplast Transformation by Ti Plasmid—Whole Plants and Progeny

    I. Introduction

    II. Crown Gall Induction on Plants

    III. T-DNA Characteristics

    IV. Tumor Morphology Mutants

    V. Plant Cell Transformation in Vitro

    VI. The Fate of T-DNA in Regenerated Plants

    VII. Concluding Remarks


    Methods and Mechanisms of Gene Uptake in Protoplasts

    I. Introduction

    II. Uptake of Isolated Genes

    III. Direct Gene Transfer from Bacteria to Plant Cells

    IV. Are Microtubules Involved in Gene Transfer

    V. Conclusions


    Protoplast Technology Applied to Metabolite Production

    I. Introduction

    II. Metabolite Synthesis

    III. Metabolite Accumulation

    IV. Clonal Variation in Metabolite Production

    V. Somatic Inheritance of Metabolite Production

    VI. Cytology of Metabolite Production

    VII. Conclusion


    Higher Plant Protoplasts—Retrospect and Prospect

    I. Introduction

    II. Aspects of Protoplast Isolation and Culture

    III. Morphological Studies of Isolated Protoplasts

    IV. Physiological Studies with Plant Protoplasts

    V. Genetic Manipulations with Plant Protoplasts

    VI. Concluding Remarks



Product details

  • No. of pages: 320
  • Language: English
  • Copyright: © Academic Press 1983
  • Published: January 1, 1983
  • Imprint: Academic Press
  • eBook ISBN: 9781483218397

About the Editor

Kenneth L. Giles

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