The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.
Plant biochemists, plant molecular biologists, plant physiologists, agriculturists, and plant microbiologists.
Cells, Protoplasts, and Liposomes: Techniques of Cell Suspension Culture. Culture and Characteristics of Green Plant Cells. Preparation of Protoplasts from Plant Tissues for Organelle Isolation. Interaction of Plant Protoplast and Liposome. Liposomes as Carriers for the Transfer and Expression of Nucleic Acids into Higher Plant Protoplasts. Interspecific Transfer of Partial Nuclear Genomic Information by Protoplast Fusion.
Vacuoles and Tonoplasts: Isolation of Mature Vacuoles of Higher Plants: General Principles, Criteria for Purity and Integrity. Isolation of Vacuoles and Tonoplast from Protoplasts. Preparation of Tonoplast Vesicles from Isolated Vacuoles. Biochemical and Enzymatic Components of a Vacuolar Membrane: Tonoplast of Lutoids from Hevea Latex. Characterization of Tonoplast Enzyme Activities and Transport. Preparation of Tonoplast Vesicles: Applications to H+-Coupled Secondary Transport in Plant Vacuoles. Purification and Characterization of the Tonoplast H+-Translocating ATPase. High-Performance Liquid Chromatography for Simultaneous Kinetic Measurements of Adenine Nucleotides in Isolated Vacuoles.
Plastids: Isolation of Intact Chloroplasts: General Principles and Criteria of Integrity. Isolation of Plastids in Density Gradients of Percoll and Other Silica Sols. Purification of Chloroplasts Using Silica Sols. Separation of Chloroplasts and Cytosol from Protoplasts. Use of Thermolysin to Probe the Cytosolic Surface of the Outer Envelope Membrane from Plastids. Characterization of Plastid Polypeptides from the Outer and Inner Envelope Membranes. Isolation of Amyloplasts from Suspension Cultures of Soybean.<BR id=
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- © Academic Press 1987
- 19th November 1987
- Academic Press
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- Hardcover ISBN:
@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be Colowick and Kaplan's multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY