Cells, Protoplasts, and Liposomes: Techniques of Cell Suspension Culture. Culture and Characteristics of Green Plant Cells. Preparation of Protoplasts from Plant Tissues for Organelle Isolation. Interaction of Plant Protoplast and Liposome. Liposomes as Carriers for the Transfer and Expression of Nucleic Acids into Higher Plant Protoplasts. Interspecific Transfer of Partial Nuclear Genomic Information by Protoplast Fusion.
Vacuoles and Tonoplasts: Isolation of Mature Vacuoles of Higher Plants: General Principles, Criteria for Purity and Integrity. Isolation of Vacuoles and Tonoplast from Protoplasts. Preparation of Tonoplast Vesicles from Isolated Vacuoles. Biochemical and Enzymatic Components of a Vacuolar Membrane: Tonoplast of Lutoids from Hevea Latex. Characterization of Tonoplast Enzyme Activities and Transport. Preparation of Tonoplast Vesicles: Applications to H+-Coupled Secondary Transport in Plant Vacuoles. Purification and Characterization of the Tonoplast H+-Translocating ATPase. High-Performance Liquid Chromatography for Simultaneous Kinetic Measurements of Adenine Nucleotides in Isolated Vacuoles.
Plastids: Isolation of Intact Chloroplasts: General Principles and Criteria of Integrity. Isolation of Plastids in Density Gradients of Percoll and Other Silica Sols. Purification of Chloroplasts Using Silica Sols. Separation of Chloroplasts and Cytosol from Protoplasts. Use of Thermolysin to Probe the Cytosolic Surface of the Outer Envelope Membrane from Plastids. Characterization of Plastid Polypeptides from the Outer and Inner Envelope Membranes. Isolation of Amyloplasts from Suspension Cultures of Soybean. Isolation of Amyloplasts from Developing Endosperm of Maize (Zea mays L.). Isolation of Plastids from Buds of Cauliflower (Brassica oleracea L.). Isolation of Membranous Chromoplasts from Daffodil Flowers. Structure and Function of the Inner Membrane Systems in Etioplasts. Isolation Procedures for Inside-Out ThylakoidVesicles. Characterization of Chloroplast Cytochromes. Cell-Free Reconstitution of Protein Transport into Chloroplasts. Polar Lipids of Chloroplast Membranes. Isolation and Reconstitution of Thylakoid Lipids. Long-Chain Fatty Acid Synthesis and Utilization by Isolated Chloroplasts. Phosphatidylglycerol Synthesis in Chloroplast Membranes. Galactolipid Biosynthesis in Chloroplast Membranes. Chlorophylls and Carotenoids: Pigments of Photosynthetic Biomembranes.
a-Tocopherol and Plastoquinone Synthesis in Chloroplast Membranes. Solubilization and Reconstitution of Carotenogenic Enzymes from Daffodil Chromoplast Membranes Using 3-[(3-Cholamidopropyl)dimethylammonio]-1-propane Sulfonate.
Mitochondria: Isolation of Plant Mitochondria: General Principles and Criteria of Integrity. Purification of Plant Mitochondria on Silica Sol Gradients. Isolation of Mitochondria from Leaves of C3, C4, and Crassulacean Acid Metabolism Plants. Separation of Spinach Leaf Mitochondria According to Surface Properties: Partition in Aqueous Polymer Two-Phase Systems. Isolation of Submitochondrial Particles with Different Polarities. Isolation of the Outer Membrane of Plant Mitochondria. Characterization of Channels Isolated from Plant Mitochondria. Phosphoglycerides of Mitochondrial Membranes. Ubiquinone Biosynthesis in Plant Mitochondria. Purification of Complexes II and IV from Plant Mitochondria.
Peroxisomes and Glyoxysomes: Isolation of Glyoxysomes and Purification of Glyoxysomal Membranes. Peroxisomes and Fatty Acid Degradation. Proteins and Phospholipids of Glyoxysomal Membranes from Castor Bean.
Nuclei, Endoplasmic Reticulum, and Plasma Membrane: Isolation of Nuclei from Soybean Suspension Cultures. Isolation of the Plasma Membrane: Membrane Markers and General Principles. Preparation of High-Purity Plasma Membranes. Possible Approaches to Surface Labeling of the Plasma Membrane. Isolation of Endoplasmic Reticulum: General Principles, Enzymatic Markers, and Endoplasmic Reticulum-Bound Polysomes. Phosphoglyceride Synthesis in Endoplasmic Reticulum.
General Physical and Biochemical Methods: Electron Microscopy of Plant Cell Membranes. Two-Dimensional Electrophoresis in the Analysis and Preparation of Cell Organelle Polypeptides. Plant Membrane Sterols: Isolation, Identification, and Biosynthesis. Separation of Molecular Species of Plant Glycolipids and Phospholipids by High-Performance Liquid Chromatography. Movement of Phospholipids between Membranes: Purification of a Phospholipid Transfer Protein from Spinach Leaf. Pesticides and Lipid Synthesis in Plant Membranes. Rapid Filtration Technique for Metabolite Fluxes across Cell Organelles. Application of Nuclear Magnetic Resonance Methods to Plant Tissues. Author Index. Subject Index.
The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.
Plant biochemists, plant molecular biologists, plant physiologists, agriculturists, and plant microbiologists.
- No. of pages:
- © Academic Press 1987
- 19th November 1987
- Academic Press
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Vanderbilt University School of Medicine, Nashville, Tennessee, U.S.A.
Helmut Sies is an Honorary Member of the American Society for Biochemistry and Molecular Biology. He received an Honorary Ph.D. from the University of Buenos Aires, Argentina in 1996. Dr. Sies is a member of the Northrhine-Westphalian Academy of Sciences, Germany, and a Corresponding Member of both the Academy of Sciences of Heidelberg, Germany, and the Academy of Medicine, Buenos Aires, Argentina. He has received many awards and prizes, including the FEBS Anniversary Prize awarded by the Federation of European Biochemical Societies, 1978; the Distinguished Foreign Scholar award, MASUA, 1985; the Silver Medal, Karolinska Institute, Stockholm, 1986; the Ernst Jung Preis fur Medizin, 1988; the Claudius-Galenus-Preis, 1990; and the ISFE-Preis, 1994. Dr. Sies sereves on the editorial board and advisory committee for twelve journals, has edited numerous books, and has published more than 400 original articles and chapters. He received his M.D. at the University of Munich in 1967 and currently serves as Full Professor and Chairman of the Department of Physiological Chemistry at the University of Düsseldorf.
Institut Fur Physiologische Chemie, Germany
Université de Grenoble, France