Physical Principles and Techniques of Protein Chemistry Part C

1st Edition - January 1, 1973
Editor: Sydney Leach
Language: English
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 1 4 1 1 4 - 7

Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from… Read more

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Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry.

List of Contributors

Preface

Contents of Other Parts

17. Density and Volume Change Measurements

Glossary of Symbols

I. Introduction

II. Definitions and Basic Considerations

III. Direct Volume Change

IV. Density

Appendix A: Derivation of the Partial Specific Volume from the Density

Appendix B: Splitting-Up of the Parameter: Exclusion of Diffusible Components

References

18. Osmotic Pressure

Glossary of Symbols

I. Introduction and Phenomenon

II. Elementary Theory

III. Applications

IV. Techniques

References

19. Small-Angle X-Ray Scattering

Glossary of Symbols

I. Introduction

II. Remarks on the Theory of Particulate Scattering

III. Remarks on the Experimental Technique

IV. Selected Examples of Applications

V. Prospects

References

20. Pulsed Nuclear Magnetic Resonance

Glossary of Symbols

I. Introduction

II. Scope of the Technique

III. Theory of Relaxation

IV. Experimental Methods

V. Experimental Design

VI. Individual Enzymes

VII. Absorption of Water by Diamagnetic Molecules

References

21. The Use of Least Squares in Data Analysis

Glossary of Symbols

I. Introduction

II. Outline of Theory

III. The Iteration Process

IV. Statistical Aspects

V. Model Functions

VI. Computational Procedure

VII. Applications

References

22. Optical Rotatory Dispersion and the Main Chain Conformation of Proteins

Glossary of Symbols

I. Introduction

II. The Basic Relations for Optically Active Molecules

III. Visible Rotatory Dispersion

IV. Peptide Cotton Effects

V. Cotton Effects Due to Side-Chain Chromophores

VI. Other Secondary Structures

VII. Conformational Transitions

VIII. Experimental Considerations

IX. The Relative Advantages of ORD and CD

Appendix: Table I

References

23. Circular Dichroism

Glossary

I. Introduction

II. Theory of Optical Activity and Its Applications

III. Secondary Structure of Proteins

IV. Side-Chain Optical Activity in Model Compounds and Proteins

V. Selected Proteins

VI. Concluding Remarks

Appendix: Electromagnetic Units

References

Author Index

Subject Index