Physical Chemical Techniques - 2nd Edition - ISBN: 9781483167411, 9781483194899

Physical Chemical Techniques

2nd Edition

Physical Techniques in Biological Research

Editors: Dan H. Moore
eBook ISBN: 9781483194899
Imprint: Academic Press
Published Date: 1st January 1968
Page Count: 404
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Physical Techniques in Biological Research, Volume II, Part A: Physical Chemical Techniques focuses on physical chemical techniques that have been most widely used in the study of molecules of biological significance. This book outlines the theoretical basis of the methods, describes the apparatus and manipulations used, and describes the applications of the techniques by examples. Organized into seven chapters, this volume begins with an overview of the basic property that makes the use of isotopes as tracers possible. This text then explains the predicted behavior during separations of chemically reacting systems by digital computer techniques. Other chapters consider the mutual diffusion in a binary system of components A and B. This book discusses as well the migration of charged particles or molecules in a liquid medium under the influence of an applied electric field. The final chapter deals with the basic units of electric potential differences. This book is a valuable resource for biological chemists.

Table of Contents

List of Contributors Preface to First Edition of Volume II Preface to Second Edition of Volume II Chapter 1 Tracer Techniques: Stable and Radioactive Isotopes I. Introduction II. Elements of Radioactivity III. The Measurement of Radioactivity IV. Radioautography V. Safe Handling of Radioactive Isotopes VI. The Measurement of the Heavy Stable Isotopes VII. The Useful Tracer Isotopes and Their Properties VIII. The Synthesis of Labeled Compounds IX. The Scope of the Tracer Technique X. Some Limitations of the Tracer Technique References Chapter 2 Ultracent Rifugation I. Introduction II. Molecular Weight Determinations III. Sedimentation Velocity IV. Density Gradient Sedimentation V. Hydrostatic Pressure Effects Reference Chapter 3 Diffusion in Liquids
I. Diffusion—a Stochastic Process II. Experimental Methods II. Diffusiometry with Astigmatic Rayleigh Interference Optics IV. Applications References Chapter 4 Electrophoresis I. Introduction II. Microscopic Method III. Early Moving Boundary Apparatus IV. The Tiselius Apparatus V. Optical Systems VI. Electrophoretic Procedure VII. The Measurement of Conductivity and Calculation of Mobility VIII. Some Hints on Interpretation of Patterns IX. Applications of Moving Boundary Electrophoresis X. Separatory Electrophoresis Techniques References Chapter 5 Electrophoresis of Proteins and Peptides in Stabilizing Media I. Introduction II. Theoretical Considerations on Electrophoresis in Stabilizing Media III. Description of the Supporting Media IV. Special Methods References Chapter 6 Surface Film Techniques I. Films at the Air-Water Interface II. Interfacial Films III. Transfer of Surface Films from a Liquid onto a Solid Surface IV. Thickness of Surface Films V. Sur


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© Academic Press 1968
Academic Press
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About the Editor

Dan H. Moore

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