Photoacoustic Probes for In Vivo Imaging

1st Edition, Volume 657 - August 3, 2021
Editor: Jefferson Chan
Language: English
Hardback ISBN:
9 7 8 - 0 - 3 2 3 - 8 5 5 3 0 - 3
eBook ISBN:
9 7 8 - 0 - 3 2 3 - 8 5 5 3 1 - 0

Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of aut… Read more

Photoacoustic Probes for In Vivo Imaging

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Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters. Each chapter is written by an international board of authors.

Cover image
Title page
Table of Contents
Copyright
Contributors
Preface
Chapter One: Development of a small-molecule-based activatable photoacoustic probe
Abstract
1: Introduction
2: Development of small-molecule-based activatable PA probes
3: Development of an activatable PA probe for HOCl: PA-MMSiNQ
4: Protocols
5: Concluding remarks
Chapter Two: A caspase-3 activatable photoacoustic probe for in vivo imaging of tumor apoptosis
Abstract
1: Introduction
2: Design of tumor targeting and caspase-3 activatable probe, 1-RGD
3: Synthesis of 1-RGD and control probe 1-Ac
4: In vitro characterization of 1-RGD and 1-Ac
5: In vivo application of 1-RGD
6: Summary
Chapter Three: Quantitatively visualizing the activity of MMP-2 enzyme in vivo using a ratiometric photoacoustic probe
Abstract
1: Introduction
2: Design and construction of MMP-2-responsive and FRET-based probe
3: Synthesis of QSY21-GPLGVRGY-Cy5.5 (QC) and control probe QSY21-GLALGPGY-Cy5.5
4: In vitro characterization of QSY21-GPLGVRGY-Cy5.5 (QC) and control QSY21-GLALGPGY-Cy5.5
5: Hydrodynamic size profile and temporal evolution of the QC probe particles in PBS and DMEM
6: Photophysical characterization of QSY21-NHS, Cy5.5-N3 and QC
7: In vitro enzyme test
8: Morphology study
9: PA property study of probes
10: Cell culture
11: Confocal
12: Mice tumor model
13: In vivo SPECT imaging
14: In vivo photoacoustic imaging
15: In vitro determination of MMP-2 expression
16: Concluding remarks
Chapter Four: Aluminum naphthalocyanine conjugate as an MMP-2-activatable photoacoustic probe for in vivo tumor imaging
Abstract
1: Introduction
2: Selection of photoacoustic photosensitizer
3: Design of MMP-2-responsive photoacoustic probe AlNc-pep-PEG
4: Synthesis of AlNc-pep-PEG
5: In vitro characterization of AlNc-pep-PEG
6: Characterization of AlNc-pep-PEG in vivo
7: Concluding remarks
Chapter Five: Alkaline phosphatase-triggered self-assembly of near-infrared nanoparticles for the enhanced photoacoustic imaging of tumors
Abstract
1: Introduction
2: Design of 1P and 1
3: Syntheses of 1P and 1
4: In vitro characterizations of 1P and 1
5: In vivo characterizations of 1P
6: Concluding remarks
Chapter Six: A TME-activated in situ nanogenerator for magnetic resonance/fluorescence/photoacoustic imaging
Abstract
1: Introduction
2: Materials
3: Methods
4: Notes
5: Results
6: Summary
Acknowledgment
Chapter Seven: Near-infrared II photoacoustic probes for nitric oxide sensing
Abstract
1: Introduction
2: Strategy for optimizing nitric oxide response
3: Selection of nitric oxide responsive trigger
4: Design of phase two probes
5: Synthesis of phase two probes
6: Photophysical characterization of phase two probes
7: Selection of APNO-1080 and in vitro characterization
8: In vivo characterization of APNO-1080
9: Concluding remarks
Chapter Eight: Monitoring tumor growth with a novel NIR-II photoacoustic probe
Abstract
1: Introduction
2: Before you begin
3: Key resources table
4: Design and synthesis of BDT-TQ
5: Photophysical characterization of BDT-TQ in vitro
6: Characterization of BDT-TQE-Tat nanoparticles in vivo
7: Conclusion
Chapter Nine: A high-contrast photoacoustic agent with near-infrared emission
Abstract
1: Introduction
2: Preparation and properties of fluorescent agent IR-1302 NPs
3: Optical characterization of IR-1302 NPs in vitro
4: Photothermal functionality of IR-1302 NPs in vitro
5: Photoacoustic imaging of IR-1302 NPs in vitro
6: Photoacoustic imaging of IR-1302 NPs in vivo
7: Concluding remarks
Chapter Ten: Mitochondria-targeted photoacoustic probe for imaging of hydrogen peroxide in inflamed mouse model
Abstract
1: Introduction
2: Design of TPP-HCy-BOH and HCy-BOH
3: Syntheses of TPP-HCy-BOH and HCy-BOH
4: Characterization of TPP-HCy-BOH and HCy-BOH in vitro
5: Characterization of TPP-HCy-BOH and HCy-BOH in vivo
6: Conclusions
Chapter Eleven: Fluoro-photoacoustic polymeric renal reporter for real-time dual imaging of acute kidney injury
Abstract
1: Introduction
2: Selection of biomarkers for early diagnosis of acute kidney injury
3: Design of FPRR
4: Synthesis of FPRR
5: In vitro characterization of FPRR
6: In vivo characterization of FPRR
7: Conclusions
Chapter Twelve: ALP-activated probe for diagnosis of liver injury by multispectral optoacoustic tomography
Abstract
1: Introduction
2: Construction of activatable photoacoustic probes for detection of specific biomarkers
3: Design of the ALP-activated small molecule photoacoustic probe (C1X-OR1)
4: Synthesis of the probe C1X-OR1 and the hepatocyte-targeting phospholipid DSPE-PEG2000-ChA
5: Response of C1X-OR1 toward ALP in solution
6: Response of C1X-OR1 toward ALP in cells
7: In vivo detection of ALP for imaging drug-induced liver injury by use of liposomal C1X-OR1
8: Summary
Acknowledgment
Chapter Thirteen: Photoacoustic probe of targeting intracellular Staphylococcus aureus infection with signal-enhanced by self-assembly
Abstract
1: Introduction
2: The mechanism of PA probes (MPC)
3: Before you begin
4: Key resources table
5: Materials and equipment
6: Step-by-step method details
7: Expected outcomes
8: Quantification and statistical analysis
9: Advantages
10: Limitations
11: Optimization and troubleshooting
12: Safety considerations and standards
13: Alternative methods/procedures
14: Concluding remarks
Chapter Fourteen: Bioengineered bacterial vesicles for optoacoustics-guided phototherapy
Abstract
1: Introduction
2: Protocol
3: Summary
Acknowledgments
Chapter Fifteen: A practical guide to photoswitching optoacoustics tomography
Abstract
1: Introduction
2: General experimental considerations
3: Data preprocessing
4: “Manual” data analysis
5: Data analysis using ML
6: Multiplexing
7: Pitfalls and troubleshooting
Acknowledgments
Funding
Chapter Sixteen: Oxygen-embedded quinoidal acene based semiconducting chromophore nanoprobe for amplified photoacoustic imaging
Abstract
1: Introduction
2: Design of Nano(O-Nonacene)-PEG and Nano([email protected])-PEG
3: Synthesis of Nano(O-Nonacene)-PEG
4: Characterization of Nano(O-Nonacene)-PEG
5: Characterization of Nano(O-Nonacene)-PEG in vivo
6: Concluding remarks
Chapter Seventeen: A general strategy to optimize the performance of aza-BODIPY-based probes for enhanced photoacoustic properties
Abstract
1: Introduction
2: Strategy for optimization of aza-BODIPY platform by conformational restrictions
3: Selection of conformationally restricted aza-BODIPY platform
4: Design of CRaB probes
5: Synthesis of CRaB probes
6: In vitro characterization of CRaB analogs
7: In vivo characterization of CRaB-Hyp
8: Concluding remarks
Chapter Eighteen: Functionalized contrast agents for multimodality photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy molecular retinal imaging
Abstract
1: Introduction
2: Requirements of ophthalmology exogenous contrast agent design
3: Multimodality PAM, OCT, and fluorescence imaging
4: Physical production of ultrapure spherical colloidal GNPs
5: Synthesis of indocyanine green (ICG)-labeled and Arginine(R)-Glycine(G)-Aspartic(D) (RGD) peptide-conjugated CGNP clusters (ICG@CGNP clusters-RGD)
6: Characterization of CGNP clusters
7: In vitro PAM and OCT evaluation of CGNP clusters-RGD and photostability analysis
8: In vivo multimodal PAM and OCT retinal molecular imaging
9: Conclusions

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Jefferson Chan