This volume provides up-to-date information on the purification and characterization of the enzymes involved in phospholipid biosynthesis and on the mechanisms utilized by cells for the transfer of phospholipids. It also includes protocols for generating phospholipid biosynthetic mutants.


Biochemists, molecular biologists, microbiologists, and researchers in the pharmaceutical industry.

Table of Contents

E.A. Dennis, The Biosynthesis of Phospholipids. Strategies for Generating Phospholipid Synthesis Mutants: W. Dowhan, Strategies for Generating and Utilizing Phospholipid Synthesis Mutants in Escherichia coli. M.J. Swede, K.A. Hudak, J.M. Lopes, and S.A. Henry, Strategies for Generating Phospholipid Synthesis Mutants in Yeast. R.A. Zoeller and C.R.H. Raetz, Strategies for Isolating Somatic Cell Mutants Defective in Lipid Biosynthesis of Plasmalogens. Acyltransferases: M.A. Scheideler and R.M. Bell, Glycerophosphate Acyltransferase from Escherichia coli. D. Haldar and A. Vancura, Glycerophosphate Acyltransferase from Liver. T. Sugiura and K. Waku, Coenzyme A-Independent Acyltransferase. P.C. Choy, P.G. Tardi, and J.J. Mukherjee, Lysophosphatidylcholine Acyltransferase. P.C. Choy and C.R. McMaster, 1-Alkyl- and 1-Alkenylglycerophosphocholine Acyltransferases. K.O. Webber and A.K. Hajra, Dihydroxyacetone Phosphate Acyltransferase. R.A. Coleman, Diacylglycerol Acyltransferase and Monoacylglycerol Acyltransferase from Liver and Intestine. K.Y. Hostetler, S.J. Huterer, and J.R. Wherrett, Biosynthesis of Bis(monoacylglycero)phosphate in Liver and Macrophage Lysosomes. S. Jackowski, L. Hsu, and C.O. Rock, 2-Acylglycerophosphoethanolamine Acyltransferase/Acyl-[Acyl-Carrier-Protein] Synthetase from Escherichia coli. Kinases: K. Ishidate and Y. Nakazawa, Choline/Ethanolamine Kinase from Rat Kidney. T.J. Porter and C. Kent, Choline/Ethanolamine Kinase from Rat Liver. T. Uchida and S. Yamashita, Choline/Ethanolamine Kinase from Rat


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© 1992
Academic Press
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