Description

This volume provides up-to-date information on the purification and characterization of the enzymes involved in phospholipid biosynthesis and on the mechanisms utilized by cells for the transfer of phospholipids. It also includes protocols for generating phospholipid biosynthetic mutants.

Readership

Biochemists, molecular biologists, microbiologists, and researchers in the pharmaceutical industry.

Table of Contents

E.A. Dennis, The Biosynthesis of Phospholipids. Strategies for Generating Phospholipid Synthesis Mutants: W. Dowhan, Strategies for Generating and Utilizing Phospholipid Synthesis Mutants in Escherichia coli. M.J. Swede, K.A. Hudak, J.M. Lopes, and S.A. Henry, Strategies for Generating Phospholipid Synthesis Mutants in Yeast. R.A. Zoeller and C.R.H. Raetz, Strategies for Isolating Somatic Cell Mutants Defective in Lipid Biosynthesis of Plasmalogens. Acyltransferases: M.A. Scheideler and R.M. Bell, Glycerophosphate Acyltransferase from Escherichia coli. D. Haldar and A. Vancura, Glycerophosphate Acyltransferase from Liver. T. Sugiura and K. Waku, Coenzyme A-Independent Acyltransferase. P.C. Choy, P.G. Tardi, and J.J. Mukherjee, Lysophosphatidylcholine Acyltransferase. P.C. Choy and C.R. McMaster, 1-Alkyl- and 1-Alkenylglycerophosphocholine Acyltransferases. K.O. Webber and A.K. Hajra, Dihydroxyacetone Phosphate Acyltransferase. R.A. Coleman, Diacylglycerol Acyltransferase and Monoacylglycerol Acyltransferase from Liver and Intestine. K.Y. Hostetler, S.J. Huterer, and J.R. Wherrett, Biosynthesis of Bis(monoacylglycero)phosphate in Liver and Macrophage Lysosomes. S. Jackowski, L. Hsu, and C.O. Rock, 2-Acylglycerophosphoethanolamine Acyltransferase/Acyl-[Acyl-Carrier-Protein] Synthetase from Escherichia coli. Kinases: K. Ishidate and Y. Nakazawa, Choline/Ethanolamine Kinase from Rat Kidney. T.J. Porter and C. Kent, Choline/Ethanolamine Kinase from Rat Liver. T. Uchida and S. Yamashita, Choline/Ethanolamine Kinase from Rat

Details

No. of pages:
584
Language:
English
Copyright:
© 1992
Published:
Imprint:
Academic Press
eBook ISBN:
9780080883236
Print ISBN:
9780121821104

About the serial-volume-editors

Edward Dennis

Edward A. Dennis: University of California, San Diego Distinguished Professor of Chemistry and Biochemistry and former Department Chair and Professor of Pharmacology in the School of Medicine, Dennis is currently Editor-in-Chief of the Journal of Lipid Research and Director of the LIPID MAPS initiative. He also served as Chair of the Board of Directors of the Keystone Symposia on Molecular and Cellular Biology and current as an Emeritus member of the Board of Directors. His current research focus is on phospholipases, cell signaling and lipidomics on which he has published over 300 papers, most related to signal transduction by lipid messengers

Affiliations and Expertise

University of California at San Diego, La Jolla, CA, U.S.A.

Dennis Vance

Affiliations and Expertise

Lipid and Lipoprotein Research Group, University of Alberta, Edmonton, Canada

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY