Phosphatases - 1st Edition - ISBN: 9780128138816, 9780128138823

Phosphatases, Volume 607

1st Edition

Serial Volume Editors: Karen Allen
eBook ISBN: 9780128138823
Hardcover ISBN: 9780128138816
Imprint: Academic Press
Published Date: 23rd August 2018
Page Count: 440
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Table of Contents

    1. Empirical Valence Bond Simulations of Organophosphate Hydrolysis: Theory and Practice
    2. Miha Purg and Shina Caroline Lynn Kamerlin

    3. Analysis of Phosphoryl-Transfer Enzymes With QM/MM Free Energy Simulations
    4. Daniel Roston, Xiya Lu, Dong Fang, Darren Demapan and Qiang Cui

    5. Defining Dynamics of Membrane-Bound Pyrophosphatases by Experimental and Computational Single-Molecule FRET
    6. Steven P.D. Harborne, Jannik Strauss, Ainoleena Turku, Matthew A. Watson, Roman Tuma, Sarah A. Harris and Adrian Goldman

    7. A Simple Strategy to Determine the Dependence of Membrane-Bound Pyrophosphatases on K+ as a Cofactor
    8. Jannik Strauss, Craig Wilkinson, Keni Vidilaseris, Steven P.D. Harborne and Adrian Goldman

    9. Crystallization of Liganded Phosphatases in the HAD Superfamily
    10. Christine Harvey, Katherine O’Toole and Karen N. Allen

    11. Structure, Mechanism, and Substrate Profiles of the Trinuclear Metallophosphatases From the Amidohydrolase Superfamily
    12. Swapnil V. Ghodge and Frank M. Raushel

    13. Affinities and Comparisons of Enzyme States by Principal Component Analysis of NMR Spectra, Automated Using TREND Software
    14. Jia Xu and Steven R. Van Doren

    15. Assessment and Impacts of Phosphorylation on Protein Flexibility of the α-D-Phosphohexomutases
    16. Kyle M. Stiers and Lesa J. Beamer

    17. Chemical Tools for Studying the Impact of cis/trans Prolyl Isomerization on Signaling: A Case Study on RNA Polymerase II Phosphatase Activity and Specificity
    18. Nathaniel Tate Burkholder, Brenda Medellin, Seema Irani, Wendy Matthews, Scott A. Showalter and Yan Jessie Zhang

    19. Quantitative Analysis of Intracellular Response Regulator Phosphatase Activity of Histidine Kinases
    20. Rong Gao and Ann M. Stock

    21. Measuring the Activities of Two-Component Regulatory System Phosphatases
    22. Robert B. Bourret and Ruth E. Silversmith

    23. Assembly of PGAM5 Into Multimeric Complexes Provides a Mechanism for Allosteric Regulation of Phosphatase Activity
    24. Peter Tipton, Tong Su and Mark Hannink

    25. Purification of Lipin and Measurement of Phosphatidic Acid Phosphatase Activity From Liposomes
    26. Mitchell E. Granade and Thurl E. Harris

    27. Combining SAMDI Mass Spectrometry and Peptide Arrays to Profile Phosphatase Activities
    28. Lindsey C. Szymczak, Che-Fan Huang, Eric J. Berns and Milan Mrksich

    29. Protein Chemical Approaches to Understanding PTEN Lipid Phosphatase Regulation

Daniel R. Dempsey and Philip A. Cole


Methods in Enzymology, Volume 607: Phosphatases, the latest release in this ongoing series, highlights new advances in the field as detailed by an international board of authors. This latest release includes chapters on Empirical Valence Bond Simulations of the Evolution of Enzyme Function, QM/MM Free Energy and Kinetic Isotope Analysis of Phosphoryl Transfer in Enzymes, the Structural, Mechanism and Evolution of Phosphatases, How to Define Rapid Motions in Pumping Pyrophosphatases, The Evolution of K+-Independence in Pyrophosphatases, the Crystallization of Michaelis, Intermediate and Inhibited Complexes in Phosphatases, and an Investigation of Nucleotide Loading and Effector Binding of K-Ras.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Updated release includes the latest information on phosphatases


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


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© Academic Press 2018
Academic Press
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Ratings and Reviews

About the Serial Volume Editors

Karen Allen Serial Volume Editor

Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering

Affiliations and Expertise

Department of Chemistry, Metcalf Center for Science and Engineering, Boston University, Boston, MA, USA