1st Edition - August 23, 2018

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  • Editor: Karen Allen
  • eBook ISBN: 9780128138823
  • Hardcover ISBN: 9780128138816

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Methods in Enzymology, Volume 607: Phosphatases, the latest release in this ongoing series, highlights new advances in the field as detailed by an international board of authors. This latest release includes chapters on Empirical Valence Bond Simulations of the Evolution of Enzyme Function, QM/MM Free Energy and Kinetic Isotope Analysis of Phosphoryl Transfer in Enzymes, the Structural, Mechanism and Evolution of Phosphatases, How to Define Rapid Motions in Pumping Pyrophosphatases, The Evolution of K+-Independence in Pyrophosphatases, the Crystallization of Michaelis, Intermediate and Inhibited Complexes in Phosphatases, and an Investigation of Nucleotide Loading and Effector Binding of K-Ras.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Updated release includes the latest information on phosphatases


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. Empirical Valence Bond Simulations of Organophosphate Hydrolysis: Theory and Practice
    Miha Purg and Shina Caroline Lynn Kamerlin
    2. Analysis of Phosphoryl-Transfer Enzymes With QM/MM Free Energy Simulations
    Daniel Roston, Xiya Lu, Dong Fang, Darren Demapan and Qiang Cui
    3. Defining Dynamics of Membrane-Bound Pyrophosphatases by Experimental and Computational Single-Molecule FRET
    Steven P.D. Harborne, Jannik Strauss, Ainoleena Turku, Matthew A. Watson, Roman Tuma, Sarah A. Harris and Adrian Goldman
    4. A Simple Strategy to Determine the Dependence of Membrane-Bound Pyrophosphatases on K+ as a Cofactor
    Jannik Strauss, Craig Wilkinson, Keni Vidilaseris, Steven P.D. Harborne and Adrian Goldman
    5. Crystallization of Liganded Phosphatases in the HAD Superfamily
    Christine Harvey, Katherine O’Toole and Karen N. Allen
    6. Structure, Mechanism, and Substrate Profiles of the Trinuclear Metallophosphatases From the Amidohydrolase Superfamily
    Swapnil V. Ghodge and Frank M. Raushel
    7. Affinities and Comparisons of Enzyme States by Principal Component Analysis of NMR Spectra, Automated Using TREND Software
    Jia Xu and Steven R. Van Doren
    8. Assessment and Impacts of Phosphorylation on Protein Flexibility of the α-D-Phosphohexomutases
    Kyle M. Stiers and Lesa J. Beamer
    9. Chemical Tools for Studying the Impact of cis/trans Prolyl Isomerization on Signaling: A Case Study on RNA Polymerase II Phosphatase Activity and Specificity
    Nathaniel Tate Burkholder, Brenda Medellin, Seema Irani, Wendy Matthews, Scott A. Showalter and Yan Jessie Zhang
    10. Quantitative Analysis of Intracellular Response Regulator Phosphatase Activity of Histidine Kinases
    Rong Gao and Ann M. Stock
    11. Measuring the Activities of Two-Component Regulatory System Phosphatases
    Robert B. Bourret and Ruth E. Silversmith
    12. Assembly of PGAM5 Into Multimeric Complexes Provides a Mechanism for Allosteric Regulation of Phosphatase Activity
    Peter Tipton, Tong Su and Mark Hannink
    13. Purification of Lipin and Measurement of Phosphatidic Acid Phosphatase Activity From Liposomes
    Mitchell E. Granade and Thurl E. Harris
    14. Combining SAMDI Mass Spectrometry and Peptide Arrays to Profile Phosphatase Activities
    Lindsey C. Szymczak, Che-Fan Huang, Eric J. Berns and Milan Mrksich
    15. Protein Chemical Approaches to Understanding PTEN Lipid Phosphatase Regulation
    Daniel R. Dempsey and Philip A. Cole

Product details

  • No. of pages: 440
  • Language: English
  • Copyright: © Academic Press 2018
  • Published: August 23, 2018
  • Imprint: Academic Press
  • eBook ISBN: 9780128138823
  • Hardcover ISBN: 9780128138816

About the Serial Volume Editor

Karen Allen

Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering

Affiliations and Expertise

Department of Chemistry, Metcalf Center for Science and Engineering, Boston University, Boston, MA, USA

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