Phosphatases - 1st Edition - ISBN: 9780128138816

Phosphatases, Volume 607

1st Edition

Serial Volume Editors: Karen Allen
Hardcover ISBN: 9780128138816
Imprint: Academic Press
Published Date: 1st September 2018
Page Count: 412
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Table of Contents

1. Empirical Valence Bond Simulations of the Evolution of Enzyme Function
Shina Caroline Lynn Kamerlin and Miha Purg
2. QM/MM free energy and kinetic isotope analysis of phosphoryl transfer in enzymes
Qiang Cui

Structural, mechanism and evolution of phosphatases
3. How to define rapid motions in pumping pyrophosphatases
Adrian Goldman
4. The evolution of K+-independence in pyrophosphatases
Adrian Goldman
5. Crystallization of Michaelis, Intermediate and Inhibited Complexes in Phosphatases
Karen N. Allen
6. Investigation of Nucleotide Loading and Effector Binding of K-Ras
Vadim Gaponenko

Substrate specificity in phosphatases
7. Phosphatases from the Amidohydrolase Superfamily
Frank M. Raushel
8. Comparison of Enzyme States by Principal Component Analysis of NMR Spectra using TREND NMR Software
Steven R. Van Doren
9. Assessment and impacts of phosphorylation on protein flexibility of the phosphohexomutases
Lesa Beamer
10. Molecular tools for studying the impact of cis/trans prolyl isomerization on RNA polymerase II CTD phosphatase activity and specificity
Yan Jessie Zhang

Signaling and Regulation
11. Quantitative analysis of intracellular phosphatase activity of histidine kinases
Ann M. Stock
12. Measuring the Activities of Two-Component Regulatory System Phosphatases
Robert B. Bourret
13. Biochemical Characterization of PGAM5, an Atypical Protein Phosphatase
Peter Tipton
14. Measuring phosphatidic acid phosphatase activity of purified lipins
Thurl Harris
15. Combining SAMDI Mass Spectrometry and Peptide Arrays to Profile Phosphatase Activities
Milan Mrksich
16. Protein Chemical Approaches to Understanding PTEN Lipid Phosphatase Regulation
Phillip A. Cole


Methods in Enzymology, Volume 607: Phosphatases, the latest release in this ongoing series, highlights new advances in the field as detailed by an international board of authors. This latest release includes chapters on Empirical Valence Bond Simulations of the Evolution of Enzyme Function, QM/MM Free Energy and Kinetic Isotope Analysis of Phosphoryl Transfer in Enzymes, the Structural, Mechanism and Evolution of Phosphatases, How to Define Rapid Motions in Pumping Pyrophosphatases, The Evolution of K+-Independence in Pyrophosphatases, the Crystallization of Michaelis, Intermediate and Inhibited Complexes in Phosphatases, and an Investigation of Nucleotide Loading and Effector Binding of K-Ras.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Updated release includes the latest information on phosphatases


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


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© Academic Press 2018
Academic Press
Hardcover ISBN:

Ratings and Reviews

About the Serial Volume Editors

Karen Allen Serial Volume Editor

Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering

Affiliations and Expertise

Department of Chemistry, Metcalf Center for Science and Engineering, Boston University, Boston, MA, USA