Peptide, Protein and Enzyme Design

Peptide, Protein and Enzyme Design

1st Edition - August 27, 2016

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  • Editor: Vincent L. Pecoraro
  • eBook ISBN: 9780128054345
  • Hardcover ISBN: 9780128053805

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De Novo Enzyme Design, the newest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume includes the design of metal binding maquettes, insertion of non-natural cofactors, Cu metallopeptides, non-covalent interactions in peptide assemblies, peptide binding and bundling, heteronuclear metalloenzymes, florinated peptides, De Novo imaging agents, and protein-protein interaction.

Key Features

  • Continues the legacy of this premier serial with quality chapters on de novo enzyme design
  • Represents the newest volume in the Methods in Enzymology series, providing premier, quality chapters authored by leaders in the field
  • Ideal reference for those interested in the study of enzyme design that looks at both structure and mechanism


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. Chemical Post-Translational Modification with Designed Rhodium(II) Catalysts
    Samuel C. Martin, Matthew B. Minus and Zachary T. Ball
    2. Cell Binding Assays for Determining the Affinity of Protein-Protein Interactions: Technologies and Considerations
    Sean Hunter and Jennifer Cochran
    3. Protein and Antibody Engineering by Phage Display
    Julia C. Frei and Jonathan R. Lai
    4. Incorporation of Unnatural Amino Acids into Proteins Expressed in Mammalian Cells
    Robert Serfling and Irene Coin
    5. Method for Enzyme Design with Genetically Encoded Unnatural Amino Acids
    Cheng Hu and Jiangyun Wang
    6. Methods for Solving Highly Symmetric De Novo Designed Metalloproteins: Crystallographic Examination of a Novel Three Stranded Coiled Coil Structure Containing D-Amino Acids
    Leela Ruckthong, Jeanne A. Stuckey and Vincent L. Pecoraro
    7. SpyRings Declassified: A Blueprint for using Isopeptide-Mediated Cyclization to Enhance Enzyme Thermal Resilience
    Christopher Schoene, S. Paul Bennett and Mark Howarth
    8. Engineering and Application of LOV2-Based Photoswitches
    Seth Parker Zimmerman, Brian Kuhlman and Hayretin Yumerefendi
    9. Minimalist Design of Allosterically Regulated Protein Catalysts
    Olga V. Makhlynets and Ivan V. Korendovych
    10. Combining Design and Selection to Create Novel Protein-Peptide Interactions
    Elizabeth B. Speltz, Nicholas Sawyer and Lynne Regan
    11. Metal-Directed Design of Supramolecular Protein Assemblies
    Jake B. Bailey, Rohit H. Subramanian, Lewis A. Churchfield and F. Akif Tezcan
    12. Designing Fluorinated Proteins
    E. Neil G. Marsh
    13. Solid Phase Synthesis of Helically Folded Aromatic Oligoamides
    Simon J. Dawson, Xiaobo Hu, Stijn Claerhout and Ivan Huc
    14. Conformational Restriction of Peptides Using Dithiol Bis-Alkylation
    Leila Peraro, Timothy R. Siegert and Joshua A. Kritzer
    15. Engineering Short Pre-Organized Peptide Sequences for Metal Ion Coordination: Copper(II) a Case Study
    Luís M. P. Lima and Olga Iranzo
    16. De Novo Construction of Redox Active Proteins
    C. C. Moser, M. M Sheehan, N. M. Ennist, G. Kodali, C. Bialas, M. T. Englander, B. M. Discher and P. L. Dutton
    17. Design Strategies for Redox Active Metalloenzymes: Applications in Hydrogen Production
    Rafael Alcala-Torano, Dayn Joseph Sommer, Zahra Bahrami Dizicheh and Giovanna Ghirlanda
    18. Equilibrium Studies of Designed Metalloproteins
    Brian R. Gibney
    19. Reconstitution of Heme Enzymes with Artificial Metalloporphyrinoids
    Koji Oohora and Takashi Hayashi
    20. Creation of a Thermally Tolerant Peroxidase
    Yoshihito Watanabe and Hiroshi Nakajima
    21. Designing Covalently Linked Heterodimeric Four-Helix Bundles
    Marco Chino, Linda Leone, Ornella Maglio and Angela Lombardi
    22. Design of Heteronuclear Metalloenzymes
    Ambika Bhagi-Damodaran,Parisa Hosseinzadeh, Evan Mirts, Julian Reed, Igor D. Petrik and Yi Lu
    23. Periplasmic Screening for Artificial Metalloenzymes
    Markus Jeschek, Sven Panke and Thomas R. Ward
    24. De Novo Designed Imaging Agents Based on Lanthanide Peptides Complexes
    Anna F. A. Peacock
    25. Peptide Binding for Bio-Based Nanomaterials
    Nicholas M. Bedford, Catherine Munro and Marc R. Knecht

Product details

  • No. of pages: 684
  • Language: English
  • Copyright: © Academic Press 2016
  • Published: August 27, 2016
  • Imprint: Academic Press
  • eBook ISBN: 9780128054345
  • Hardcover ISBN: 9780128053805

About the Serial Volume Editor

Vincent L. Pecoraro

John T. Groves Collegiate Professor of Chemistry, Department of Chemistry, University of Michigan, USA

Affiliations and Expertise

Department of Chemistry, University of Michigan, MI, USA

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