Description

This volume includes sections dealing with specific growth factors in which new, updated, or alternative procedures are presented for purification, bioassay, radiolabeling and radioreceptor assay, immunoassay, receptor identification, and quantification and includes general techniques for the study of growth factors. For those unfamiliar with a particular area such as the oncogene-growth factor relationship orientation chapters have been included.

Readership

Biochemists, molecular, cell and developmental biologists, endocrinologists, neurologists, immunologists, analytical chemists, geneticists, oncologists, and physiologists.

Table of Contents

Insulin-Like Growth Factor, Nerve Growth Factor, and Platelet-Derived Growth Factor: B. Nilsson, G. Forsberg, and M. Hartmanis, Expression and Purification of Recombinant Insulin-Like Growth Factors from Escherichia coli. A. Ullrich, Insulin-Like Growth Factor I Receptor cDNA Cloning. M.A. Lesniak, L. Bassas, J. Roth, and J.M. Hill, Autoradiographic Localization of Insulin-Like Growth Factor I Receptors in Rat Brain and Chick Embryo. M.H. Tuszynski and F.H. Gage, In Vivo Assay of Neuron-Specific Effects of Nerve Growth Factor. M. Fahnestock, Detection and Assay of Nerve Growth Factor mRNA. M.V. Chao, Detection of Nerve Growth Factor Receptors after Gene Transfer. L. Claesson-Welsh, A. Eriksson, B. Westermark, and C.-H. Heldin, ~Cloning and Expression of Human Platelet-Derived Growth Factor ~ga and ~gb Receptors. L.A. Serunian, K.R. Auger, and L.C. Cantley, Identification and Quantification of Polyphosphoinositides Produced in Response to Platelet-Derived Growth Factor Stimulation. Fibroblast Growth Factor: Y. Shing, Biaffinity Chromatography of Fibroblast Growth Factors. S.A. Thompson, A.A. Protter, L. Bitting, J.C. Fiddes, and J.A. Abraham, Cloning, Recombinant Expression, and Characterization of Basic Fibroblast Growth Factor. S. Rogelj, D.F. Stern, and M. Klagsbrun, Construction and Expression of Transforming Gene Resulting from Fusion of Basic Fibroblast Growth Factor Gene with Signal Peptide Sequence. T. Yoshida, K. Miyagawa, H. Sakamoto, T. Sugimura, and M. ~Terada, Identification and Characterization of Fibroblast Growth Factor-Related Transforming Gene hst-1. J.-J. Feige and A. Baird, Phosphorylation and Identification of Phosphorylated Forms o

Details

No. of pages:
578
Language:
English
Copyright:
© 1991
Published:
Imprint:
Academic Press
Print ISBN:
9780121820992
Electronic ISBN:
9780080883137

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY