This volume includes sections dealing with specific growth factors in which new, updated, or alternative procedures are presented for purification, bioassay, radiolabeling and radioreceptor assay, immunoassay, receptor identification, and quantification and includes general techniques for the study of growth factors. For those unfamiliar with a particular area such as the oncogene-growth factor relationship orientation chapters have been included.


Biochemists, molecular, cell and developmental biologists, endocrinologists, neurologists, immunologists, analytical chemists, geneticists, oncologists, and physiologists.

Table of Contents

Insulin-Like Growth Factor, Nerve Growth Factor, and Platelet-Derived Growth Factor: B. Nilsson, G. Forsberg, and M. Hartmanis, Expression and Purification of Recombinant Insulin-Like Growth Factors from Escherichia coli. A. Ullrich, Insulin-Like Growth Factor I Receptor cDNA Cloning. M.A. Lesniak, L. Bassas, J. Roth, and J.M. Hill, Autoradiographic Localization of Insulin-Like Growth Factor I Receptors in Rat Brain and Chick Embryo. M.H. Tuszynski and F.H. Gage, In Vivo Assay of Neuron-Specific Effects of Nerve Growth Factor. M. Fahnestock, Detection and Assay of Nerve Growth Factor mRNA. M.V. Chao, Detection of Nerve Growth Factor Receptors after Gene Transfer. L. Claesson-Welsh, A. Eriksson, B. Westermark, and C.-H. Heldin, ~Cloning and Expression of Human Platelet-Derived Growth Factor ~ga and ~gb Receptors. L.A. Serunian, K.R. Auger, and L.C. Cantley, Identification and Quantification of Polyphosphoinositides Produced in Response to Platelet-Derived Growth Factor Stimulation. Fibroblast Growth Factor: Y. Shing, Biaffinity Chromatography of Fibroblast Growth Factors. S.A. Thompson, A.A. Protter, L. Bitting, J.C. Fiddes, and J.A. Abraham, Cloning, Recombinant Expression, and Characterization of Basic Fibroblast Growth Factor. S. Rogelj, D.F. Stern, and M. Klagsbrun, Construction and Expression of Transforming Gene Resulting from Fusion of Basic Fibroblast Growth Factor Gene with Signal Peptide Sequence. T. Yoshida, K. Miyagawa, H. Sakamoto, T. Sugimura, and M. ~Terada, Identification and Characterization of Fibroblast Growth Factor-Related Transforming Gene hst-1. J.-J. Feige and A. Baird, Phosphorylation and Identification of Phosphorylated Forms o


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Academic Press
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