PCR Protocols

PCR Protocols

A Guide to Methods and Applications

1st Edition - December 28, 1989

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  • Editors: Michael Innis, David Gelfand, John Sninsky, Thomas White
  • eBook ISBN: 9780080886718

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Description

The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual.

Key Features

  • Avoid contamination--with specific instructions on setting up your lab
  • Avoid cumbersome molecular biological techniques
  • Discover new applications

Readership

Researchers and technicians in molecular biology, in both basic research and applications, including genetics and disease diagnosis

Table of Contents

  • [Contents in a condensed version]
    Basic Methodology
    Amplification of genomic DNA targets
    Optimization of PCR reaction and design of primers
    Nonradioactive detection of PCR products
    mRNA quantitation by PCR
    Cloning PCR products
    Contamination and product carry-over
    PCR from paraffin sections
    Amplifying ancient DNA
    Research Applications
    Direct DNA sequencing of PCR products
    DNA footprinting
    Detection of rare mRNAs
    Site-directed mutagenesis
    Screening DNA libraries
    Amplification of flanking DNA sequences
    Genetics and Evoltion
    Tissue typing and disease susceptibility
    Haplotype analysis of single cells
    Population biology
    Multiplex DNA amplification
    Diagnostics and Forensics
    HIV detection
    HTLV I and II detection
    Screening for novel viruses
    Detection of oncogenic mutations
    Diagnosis of retinoblastoma
    Individual identification
    Instruments and Supplies
    Organizing a laboratory for PCR work
    Low-cost air-driven cycling oven
    Basic equipment and supplies for the PCR lab

Product details

  • No. of pages: 482
  • Language: English
  • Copyright: © Academic Press 1989
  • Published: December 28, 1989
  • Imprint: Academic Press
  • eBook ISBN: 9780080886718

About the Editors

Michael Innis

Affiliations and Expertise

Cetus Corporation, Emeryville, California, U.S.A.

David Gelfand

Affiliations and Expertise

Roche Molecular Systems, Alameda, California, U.S.A.

John Sninsky

Affiliations and Expertise

Roche Molecular Systems, Alameda, California, U.S.A.

Thomas White

Affiliations and Expertise

Hoffmann-Roche Diagnostic Research, Almeda, California, U.S.A.

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