PCR Protocols
A Guide to Methods and Applications
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The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual.
Key Features
- Avoid contamination--with specific instructions on setting up your lab
- Avoid cumbersome molecular biological techniques
- Discover new applications
Readership
Researchers and technicians in molecular biology, in both basic research and applications, including genetics and disease diagnosis
Table of Contents
- [Contents in a condensed version]
Basic Methodology
Amplification of genomic DNA targets
Optimization of PCR reaction and design of primers
Nonradioactive detection of PCR products
mRNA quantitation by PCR
Cloning PCR products
Contamination and product carry-over
PCR from paraffin sections
Amplifying ancient DNA
Research Applications
Direct DNA sequencing of PCR products
DNA footprinting
Detection of rare mRNAs
Site-directed mutagenesis
Screening DNA libraries
Amplification of flanking DNA sequences
Genetics and Evoltion
Tissue typing and disease susceptibility
Haplotype analysis of single cells
Population biology
Multiplex DNA amplification
Diagnostics and Forensics
HIV detection
HTLV I and II detection
Screening for novel viruses
Detection of oncogenic mutations
Diagnosis of retinoblastoma
Individual identification
Instruments and Supplies
Organizing a laboratory for PCR work
Low-cost air-driven cycling oven
Basic equipment and supplies for the PCR lab
Product details
- No. of pages: 482
- Language: English
- Copyright: © Academic Press 1989
- Published: December 28, 1989
- Imprint: Academic Press
- eBook ISBN: 9780080886718
About the Editors
Michael Innis
Affiliations and Expertise
Cetus Corporation, Emeryville, California, U.S.A.
David Gelfand
Affiliations and Expertise
Roche Molecular Systems, Alameda, California, U.S.A.
John Sninsky
Affiliations and Expertise
Roche Molecular Systems, Alameda, California, U.S.A.
Thomas White
Affiliations and Expertise
Hoffmann-Roche Diagnostic Research, Almeda, California, U.S.A.