PCR Protocols - 1st Edition - ISBN: 9780123721808, 9780080886718

PCR Protocols

1st Edition

A Guide to Methods and Applications

Editors: Michael Innis David Gelfand John Sninsky Thomas White
eBook ISBN: 9780080886718
Imprint: Academic Press
Published Date: 28th December 1989
Page Count: 482
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Description

The correct procedures you need for frustration-free PCR methods and applications are contained in this complete, step-by-step, clearly written, inexpensive manual.

Key Features

  • Avoid contamination--with specific instructions on setting up your lab
  • Avoid cumbersome molecular biological techniques
  • Discover new applications

Readership

Researchers and technicians in molecular biology, in both basic research and applications, including genetics and disease diagnosis

Table of Contents

[Contents in a condensed version] Basic Methodology Amplification of genomic DNA targets Optimization of PCR reaction and design of primers Nonradioactive detection of PCR products mRNA quantitation by PCR Cloning PCR products Contamination and product carry-over PCR from paraffin sections Amplifying ancient DNA Research Applications Direct DNA sequencing of PCR products DNA footprinting Detection of rare mRNAs Site-directed mutagenesis Screening DNA libraries Amplification of flanking DNA sequences Genetics and Evoltion Tissue typing and disease susceptibility Haplotype analysis of single cells Population biology Multiplex DNA amplification Diagnostics and Forensics HIV detection HTLV I and II detection Screening for novel viruses Detection of oncogenic mutations Diagnosis of retinoblastoma Individual identification Instruments and Supplies Organizing a laboratory for PCR work Low-cost air-driven cycling oven Basic equipment and supplies for the PCR lab

Details

No. of pages:
482
Language:
English
Copyright:
© Academic Press 1989
Published:
Imprint:
Academic Press
eBook ISBN:
9780080886718

About the Editor

Michael Innis

Affiliations and Expertise

Cetus Corporation, Emeryville, California, U.S.A.

David Gelfand

Affiliations and Expertise

Roche Molecular Systems, Alameda, California, U.S.A.

John Sninsky

Affiliations and Expertise

Roche Molecular Systems, Alameda, California, U.S.A.

Thomas White

Affiliations and Expertise

Hoffmann-Roche Diagnostic Research, Almeda, California, U.S.A.

Reviews

"For an up-to-date, definitive discussion of technical variations and the wide range of known applications, PCR Protocols, A Guide to Methods and Applications is an invaluable reference. This work must, by its nature, be definitive; the first three editors are from Cetus, the corporation... The editors have done well to give this work a surprisingly cohesive feel... This manual is an excellent summary of PCR to date. The index is good and the reference citations alone would save an investigator new to the field countless hours in the library. The prose and 'cookbook' presentation of the procedures are clear at an appropriate level for anyone with a background in more conventional molecular techniques. While PCR is in its infancy, this work will certainly be updated many times and by many others, however as a reflection of the scope of PCR to date, it will not be replaced or rendered obsolete." --APPLIED CYTOGENETICS

"PCR Protocols is an excellent lab manual for making PCR work... Most of the articles are written with numbered, step-by-step procedures and have reagent information, down to the sequences of the oligonucleotides used. Even the comb binding of the book makes it suitable for bench-top use... The breadth of the applications and methodology described in this book should give even the novice the confidence and skill to make PCR work... The 30 or so applications articles give the experienced reader a good overview of the power and utility of PCR. In summary, if you have questions about PCR, PCR Protocols probably has the answers." --TIG

"This volume owes its value to the expertise of its contributors, all specialists of international renown in the developing field of PCR... This book is an excellent compilation for all who, having a general background in molecular biology, wish to practice PCR... No user of the PCR technique could wish to miss it." --TIBTECH

"The fifty-five chapters presented in this guide to the methods and applications of PCR provide detailed experimental protocols. They are written by researchers who use PCR successfully in their laboratory and who do not shy away from pointing out the potential pitfalls. This extremely useful publication of PCR Protocols goes far beyond the simple amplification of nucleic acids... A well-organized index is provided. This book will make it more difficult to find an excuse for not using the PCR technique." --AMERICAN JOURNAL OF PHYSIOLOGY