Pathology, Vector Studies, and Culture
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Malaria: Volume 2, Pathology, Vector Studies, and Culture is a collection of papers that deals with erythrocyte destruction mechanism in malaria, the pathology of malaria, colonization of laboratory mosquitoes, and their transmission of plasmodia. Other papers describe the culture of the invertebrate stages of plasmodia, of mosquito tissues, and also of erythrocytic and exoerythrocytic stages of plasmodia. One paper constructs a model to show the roles of the different destructive and regenerative processes in the mechanisms of erythrocyte destruction in malaria. Another paper describes the organ changes and physiopathological mechanism connected with Plasmodium infection. These organs concern the spleen, liver, heart, and vascular system. One paper reviews the procedures involved in malaria transmission by mosquitoes, as well as some specialized procedures unique in avian, rodent, simian, and human malarias. Another paper discusses the benefits and drawbacks of a culture medium based on the composition of adult anopheline hemolymph. This medium should lead to a rapid growth rate in primary cultures of mosquito cells and a shorter interval of adaptation for continuous cell lines. The collection can prove useful for pharmacologists, general medical practitioners, investigators, and laboratory technicians involved in mosquito borne diseases or tropical medicine research.
Table of Contents
List of Contributors
Preface
Contents of Volumes 1 and 3
Contents of Babesiosis
1 Erythrocyte Destruction Mechanisms in Malaria
I. Introduction
II. Mechanisms of Erythrocyte Destruction
III. Proposed Model of the Relationship between Erythrocyte Loss and Parasitemia
References
2 Pathology of Malaria
I. Introduction
II. Spleen Pathology
III. Liver Pathology
IV. Heart and Vascular Pathology
V. Hematopathology
VI. Pulmonary Pathology
VII. Thymus Pathology
VIII. Renal Pathology
IX. Neuropathology
X. Placenta Pathology
References
3 Colonization and Maintenance of Mosquitoes in the Laboratory
I. Introduction
II. Basic Mosquito Biology
III. Collection of Field Material
IV. Transport of Living Material
V. Rearing
VI. Selection Procedures
VII. Pathogen and Pesticide Problems
References
4 The Transmission by Mosquitoes of Plasmodia in the Laboratory
I. Introduction
II. Susceptibility of Mosquitoes to Plasmodium
III. Gametocytes in the Vertebrate Host
IV. The Infective Blood Meal
V. Maintenance of Infected Mosquitoes
VI. Sampling Procedures for Determining the Degree of Mosquito Infection
VII. Harvesting, Purifying, and Maintaining Sporozoites
VIII. Infecting Vertebrates with Sporozoites
IX. Quantitation of Sporozoite Infectivity and Viability
X. Specific Host-Parasite-Vector Systems in Malaria Research
XI. Conclusions
References
5 Culture of the Invertebrate Stages of Plasmodia and the Culture of Mosquito Tissues
I. Introduction
II. Attempts to Culture the Vertebrate Stages of Plasmodia
III. Current Methods of Initiating Primary Cell and Organ Cultures from Mosquito Species with Emphasis on Anophelines
IV. Adult Anopheline Hemolymph Analysis and the Design of Culture Media
V. Concluding Remarks
References
6 Cultivation of Erythrocytic and Exoerythrocytic Stages of Plasmodia
I. Introduction
II. Culture of Erythrocytic Stages within Their Host Cells
III. Extracellular Culture of Erythrocytic Stages
IV. Exoerythrocytic Stages in Culture
References
Index
Product details
- No. of pages: 346
- Language: English
- Copyright: © Academic Press 1980
- Published: January 28, 1980
- Imprint: Academic Press
- eBook ISBN: 9781483258713
About the Editor
Julius P. Kreier
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