Pathology, Vector Studies, and Culture

1st Edition - January 28, 1980
Editor: Julius P. Kreier
Language: English
eBook ISBN:
9 7 8 - 1 - 4 8 3 2 - 5 8 7 1 - 3

Malaria: Volume 2, Pathology, Vector Studies, and Culture is a collection of papers that deals with erythrocyte destruction mechanism in malaria, the pathology of malaria,… Read more

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Malaria: Volume 2, Pathology, Vector Studies, and Culture is a collection of papers that deals with erythrocyte destruction mechanism in malaria, the pathology of malaria, colonization of laboratory mosquitoes, and their transmission of plasmodia. Other papers describe the culture of the invertebrate stages of plasmodia, of mosquito tissues, and also of erythrocytic and exoerythrocytic stages of plasmodia. One paper constructs a model to show the roles of the different destructive and regenerative processes in the mechanisms of erythrocyte destruction in malaria. Another paper describes the organ changes and physiopathological mechanism connected with Plasmodium infection. These organs concern the spleen, liver, heart, and vascular system. One paper reviews the procedures involved in malaria transmission by mosquitoes, as well as some specialized procedures unique in avian, rodent, simian, and human malarias. Another paper discusses the benefits and drawbacks of a culture medium based on the composition of adult anopheline hemolymph. This medium should lead to a rapid growth rate in primary cultures of mosquito cells and a shorter interval of adaptation for continuous cell lines. The collection can prove useful for pharmacologists, general medical practitioners, investigators, and laboratory technicians involved in mosquito borne diseases or tropical medicine research.

List of Contributors

Preface

Contents of Volumes 1 and 3

Contents of Babesiosis

1 Erythrocyte Destruction Mechanisms in Malaria

I. Introduction

II. Mechanisms of Erythrocyte Destruction

III. Proposed Model of the Relationship between Erythrocyte Loss and Parasitemia

References

2 Pathology of Malaria

I. Introduction

II. Spleen Pathology

III. Liver Pathology

IV. Heart and Vascular Pathology

V. Hematopathology

VI. Pulmonary Pathology

VII. Thymus Pathology

VIII. Renal Pathology

IX. Neuropathology

X. Placenta Pathology

References

3 Colonization and Maintenance of Mosquitoes in the Laboratory

I. Introduction

II. Basic Mosquito Biology

III. Collection of Field Material

IV. Transport of Living Material

V. Rearing

VI. Selection Procedures

VII. Pathogen and Pesticide Problems

References

4 The Transmission by Mosquitoes of Plasmodia in the Laboratory

I. Introduction

II. Susceptibility of Mosquitoes to Plasmodium

III. Gametocytes in the Vertebrate Host

IV. The Infective Blood Meal

V. Maintenance of Infected Mosquitoes

VI. Sampling Procedures for Determining the Degree of Mosquito Infection

VII. Harvesting, Purifying, and Maintaining Sporozoites

VIII. Infecting Vertebrates with Sporozoites

IX. Quantitation of Sporozoite Infectivity and Viability

X. Specific Host-Parasite-Vector Systems in Malaria Research

XI. Conclusions

References

5 Culture of the Invertebrate Stages of Plasmodia and the Culture of Mosquito Tissues

I. Introduction

II. Attempts to Culture the Vertebrate Stages of Plasmodia

III. Current Methods of Initiating Primary Cell and Organ Cultures from Mosquito Species with Emphasis on Anophelines

IV. Adult Anopheline Hemolymph Analysis and the Design of Culture Media

V. Concluding Remarks

References

6 Cultivation of Erythrocytic and Exoerythrocytic Stages of Plasmodia

I. Introduction

II. Culture of Erythrocytic Stages within Their Host Cells

III. Extracellular Culture of Erythrocytic Stages

IV. Exoerythrocytic Stages in Culture

References

Index