Paper Chromatography - 1st Edition - ISBN: 9781483227191, 9781483276878

Paper Chromatography

1st Edition

A Laboratory Manual

Authors: Richard J. Block Raymond Le Strange Gunter Zweig
eBook ISBN: 9781483276878
Imprint: Academic Press
Published Date: 1st January 1952
Page Count: 205
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Paper Chromatography: A Laboratory Manual focuses on methods, technologies, and processes, and aims to provide readers with a readily accessible source for the uses and adaptations of paper chromatography.
The book first offers information on general methods, including descending, ascending, and ascending-descending chromatography, filter paper ""chromatopile"", ""reversed phase"" paper chromatography, and paper electrophoresis. The text then elaborates on quantitative methods and amino acids, amines, and proteins. Discussions focus on visual comparison, elution, area of spot, total color of spot, maximum color density, identification of amines, separation of proteins, and general directions.
The publication examines carbohydrates and aliphatic acids and steroids. Topics include simple sugars, miscellaneous derived sugars, and aliphatic acids. The text also ponders on purines, pyrimidines, and related substances and phenols, aromatic acids, and porphyrins. The text is a valuable reference for readers interested in paper chromatography.

Table of Contents


I. Introduction

II. Theory

III. General Methods

Method I: Kapillaranalyse

Method II: Descending Chromatography


Chromatograph Chamber

Experimental Procedure

Method III: Ascending Chromatography

Chromatograph Chamber

Filter Paper Rack

Experimental Procedure


Method IV: Ascending Descending Chromatography

Chromatograph Chamber


Method V: Filter Paper "Chromatophile"

Method VI: "Reversed Phase" Paper Chromatography

Method VII: Circular Filter Paper Chromatography


Experimental Procedure

Identification of Zones

Method VIII: Microbiological and Enzymatic Methods

Method IX: Paper Electrophoresis

Paper Chromatography in an Electrical Field

Paper Electrophoresis

Experimental Technique

Effects of Temperature and Saturation on Paper Chromatograms

Filter Paper

Application of Sample to Paper


Application of Sample

Elution of Spots

Photoelectric Apparatus


Ultraviolet Spectrophotometer

IV. Quantitative Methods

Method I: Visual Comparison

Visual Comparison Method after Berry and Cain

Method II: Elution

Elution Technique after Fowden, Boissonas, and Block

Method III: Area of Spot


Method IV: Total Color of Spot

Total Color Density Modified from Block and Bull

Total Color Density According to Rockland


Method V: Maximum Color Density

Maximum Color Density on Two-Dimensional Paper Chromatograms

Maximum Color Density on One-Dimensional Paper Chromatograms

Method VI: Area Density Method

Method VII: Retention Analysis

V. Amino Acids, Amines and Proteins

Section I: Amino Acids and Peptides

General Directions

Section II: Identification of Dinitrophenyl Amino Acids and Amino Alcohols

Dinitrophenyl Derivatives

Amino Alcohols

Section III: Identification of Amines




Quantities Used

Color Reagents

Preparation of Sample


Section IV: Separation of Proteins

Conventional Chromatography

Paper Electrophoresis

VI. Carbohydrates

Section I: Simple Sugars

General Directions

Experimental Procedure

Section II: Di- and Polysaccharides, Starch and Cellulose

Preparation of Samples

Detection of Spots

Section III: Substituted Sugars

General Directions

Section IV: Miscellaneous Derived Sugars

Sugar Acids

Polyhydric Alcohols

VII. Aliphatic Acids and Steroids

Section I: Aliphatic Acids

General Directions

Detailed Experiment

Section II: α-Keto Acids

General Directions for α-Keto Acids

Section III: Steroids

General Directions

Detailed Experiment

VIII. Purines, Pyrimidines, and Related Substances

General Directions

Quantities Used



Techniques Used

Preparation of Samples

Detection and Quantitative Evaluation of Spots

IX. Phenols, Aromatic Acids, and Porphyrins

Section I: Phenols and Aromatic Acids

General Directions

Detailed Experiments

Section II: Porphyrins

General Directions

Detailed Experiment

X. Miscellaneous Organic Substances

Experimental Details

Adrenaline and Related Compounds

Lignin Degradation Products

Choline, Ethanolamine and Serine


Nicotinic Acid and Related Compounds

β-Indole Acetic Acid and Tryptophan

Anthraquinone Pigments

pH Indicators

Flavonoid Pigments

Chlorinated Organic Insecticides


Urea, Creatine, Creatinine, and Miscellaneous Compounds

XI. Antibiotics and Vitamins

Section I: Antibiotics

General Directions

Detailed Experiments

Section II: Vitamins

General Directions

Experimental Details

XII. Inorganic Separations

General Directions

Quantities Used



Special Techniques Used

Preparation of Samples

Detection and Determination

Experimental Details



Author Index

Subject Index


No. of pages:
© Academic Press 1952
Academic Press
eBook ISBN:

About the Author

Richard J. Block

Raymond Le Strange

Gunter Zweig

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