Paper Chromatography

Paper Chromatography

A Laboratory Manual

1st Edition - January 1, 1952

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  • Authors: Richard J. Block, Raymond Le Strange, Gunter Zweig
  • eBook ISBN: 9781483276878

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Description

Paper Chromatography: A Laboratory Manual focuses on methods, technologies, and processes, and aims to provide readers with a readily accessible source for the uses and adaptations of paper chromatography. The book first offers information on general methods, including descending, ascending, and ascending-descending chromatography, filter paper ""chromatopile"", ""reversed phase"" paper chromatography, and paper electrophoresis. The text then elaborates on quantitative methods and amino acids, amines, and proteins. Discussions focus on visual comparison, elution, area of spot, total color of spot, maximum color density, identification of amines, separation of proteins, and general directions. The publication examines carbohydrates and aliphatic acids and steroids. Topics include simple sugars, miscellaneous derived sugars, and aliphatic acids. The text also ponders on purines, pyrimidines, and related substances and phenols, aromatic acids, and porphyrins. The text is a valuable reference for readers interested in paper chromatography.

Table of Contents


  • Foreword

    I. Introduction

    II. Theory

    III. General Methods

    Method I: Kapillaranalyse

    Method II: Descending Chromatography

    Trough

    Chromatograph Chamber

    Experimental Procedure

    Method III: Ascending Chromatography

    Chromatograph Chamber

    Filter Paper Rack

    Experimental Procedure

    Comments

    Method IV: Ascending Descending Chromatography

    Chromatograph Chamber

    Comments

    Method V: Filter Paper "Chromatophile"

    Method VI: "Reversed Phase" Paper Chromatography

    Method VII: Circular Filter Paper Chromatography

    Apparatus

    Experimental Procedure

    Identification of Zones

    Method VIII: Microbiological and Enzymatic Methods

    Method IX: Paper Electrophoresis

    Paper Chromatography in an Electrical Field

    Paper Electrophoresis

    Experimental Technique

    Effects of Temperature and Saturation on Paper Chromatograms

    Filter Paper

    Application of Sample to Paper

    Micropipettes

    Application of Sample

    Elution of Spots

    Photoelectric Apparatus

    Densitometers

    Ultraviolet Spectrophotometer

    IV. Quantitative Methods

    Method I: Visual Comparison

    Visual Comparison Method after Berry and Cain

    Method II: Elution

    Elution Technique after Fowden, Boissonas, and Block

    Method III: Area of Spot

    Comment

    Method IV: Total Color of Spot

    Total Color Density Modified from Block and Bull

    Total Color Density According to Rockland

    Comment

    Method V: Maximum Color Density

    Maximum Color Density on Two-Dimensional Paper Chromatograms

    Maximum Color Density on One-Dimensional Paper Chromatograms

    Method VI: Area Density Method

    Method VII: Retention Analysis

    V. Amino Acids, Amines and Proteins

    Section I: Amino Acids and Peptides

    General Directions

    Section II: Identification of Dinitrophenyl Amino Acids and Amino Alcohols

    Dinitrophenyl Derivatives

    Amino Alcohols

    Section III: Identification of Amines

    ?? LEVELLING

    Solvents

    Paper

    Quantities Used

    Color Reagents

    Preparation of Sample

    Comments

    Section IV: Separation of Proteins

    Conventional Chromatography

    Paper Electrophoresis

    VI. Carbohydrates

    Section I: Simple Sugars

    General Directions

    Experimental Procedure

    Section II: Di- and Polysaccharides, Starch and Cellulose

    Preparation of Samples

    Detection of Spots

    Section III: Substituted Sugars

    General Directions

    Section IV: Miscellaneous Derived Sugars

    Sugar Acids

    Polyhydric Alcohols

    VII. Aliphatic Acids and Steroids

    Section I: Aliphatic Acids

    General Directions

    Detailed Experiment

    Section II: α-Keto Acids

    General Directions for α-Keto Acids

    Section III: Steroids

    General Directions

    Detailed Experiment

    VIII. Purines, Pyrimidines, and Related Substances

    General Directions

    Quantities Used

    Paper

    Solvents

    Techniques Used

    Preparation of Samples

    Detection and Quantitative Evaluation of Spots

    IX. Phenols, Aromatic Acids, and Porphyrins

    Section I: Phenols and Aromatic Acids

    General Directions

    Detailed Experiments

    Section II: Porphyrins

    General Directions

    Detailed Experiment

    X. Miscellaneous Organic Substances

    Experimental Details

    Adrenaline and Related Compounds

    Lignin Degradation Products

    Choline, Ethanolamine and Serine

    Alkaloids

    Nicotinic Acid and Related Compounds

    β-Indole Acetic Acid and Tryptophan

    Anthraquinone Pigments

    pH Indicators

    Flavonoid Pigments

    Chlorinated Organic Insecticides

    Pterins

    Urea, Creatine, Creatinine, and Miscellaneous Compounds

    XI. Antibiotics and Vitamins

    Section I: Antibiotics

    General Directions

    Detailed Experiments

    Section II: Vitamins

    General Directions

    Experimental Details

    XII. Inorganic Separations

    General Directions

    Quantities Used

    Paper

    Solvents

    Special Techniques Used

    Preparation of Samples

    Detection and Determination

    Experimental Details

    Comments

    Bibliography

    Author Index

    Subject Index


Product details

  • No. of pages: 205
  • Language: English
  • Copyright: © Academic Press 1952
  • Published: January 1, 1952
  • Imprint: Academic Press
  • eBook ISBN: 9781483276878

About the Authors

Richard J. Block

Raymond Le Strange

Gunter Zweig

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