Oxygen Radicals in Biological Systems, Part D, Volume 234

1st Edition

Print ISBN: 9780121821357
eBook ISBN: 9780080883472
Imprint: Academic Press
Published Date: 12th August 1994
Page Count: 704

Institutional Access


Since biological tissues are unstable in an oxygen atmosphere, a great deal of effort is expended by organisms to metabolically limit or repair oxidative tissue damage. This volume of Methods in Enzymology and its companion Volume 233 present methods developed to investigate the roles of oxygen radicals and antioxidants in disease. It includes techniques and assays of the activity of biothiols in soluble systems, their interaction with lipids and proteins, and the various new thiol enzymes, such as thioltransferases, being elucidated. Antioxidant reactions and gene regulation are also included.


Biochemists, pharmacologists, physiologists, and cell biologists.

Table of Contents

Oxidative Damage to DNA and DNA Repair:

M. Dizdaroglu, Chemical Determination of Oxidative DNA Damage by Gas Chromatography-Mass Spectrometry.

M.K. Shigenaga, E.N. Aboujaoude, Q. Chen, and B.N. Ames, Assays of the Oxidative DNA Damage Biomarker 8-Oxo-2-deoxyguanosine and 8-Oxoguanine in Nuclear DNA and Biological Fluids by High-Performance Liquid Chromatography with Electrochemical Detection.

K.K. Hamilton, K. Lee, and P.W. Doetsch, Detection and Characterization of Eukaryotic Enzymes That Recognize Oxidative DNA Damage.

W.A. Schulz, M.S.W. Obendorf, and H. Sies, Localization of Strand Breaks in Plasmid DNA Treated with Reactive Oxygen Species.

Y. Luo, E.S. Henle, R. Chatopadhyaya, R. Jin, and S. Linn, Detecting DNA Damage Caused by Iron and Hydrogen Peroxide.

P.K. Wong and R.A. Floyd, Photochemical Synthesis of 8-Hydroxyguanine Nucleosides.

M.J. Burkitt, Copper (DNA Adducts).

J. Cadet. J.-L. Ravanat, G.W. Buchko, H.C. Yeo, and B.N. Ames, Singlet Oxygen DNA Damage: Chromatographic and Mass Spectrometric Analysis of Damage Products.

C. Baumstark-Khan, Alkaline Elution versus Fluorescence Analysis of DNA Unwinding.

L. Augeri, K.K. Hamilton, A.M. Martin, P. Yohannes, and P.W. Doetsch, Purification and Properties of Yeast Redoxyendonuclease.

C.F.M. Menck, Shuttle Vector between Prokaryotes and Eukaryotes for Assaying Singlet Oxygen-Induced DNA Damage and Mutagenicity.

B. Epe and J. Hegler, Oxidative DNA Damage: Endonuclease Fingerprinting.

Assay of Stress Genes/Proteins, Effects of Reactive Oxygen Species and Antioxidants on Cell Regulatory Processes:

W. Drige, S. Mihm, M. Bockstette, and S. Roth, Effect of Reactive Oxygen Intermediates and An


No. of pages:
© Academic Press 1994
Academic Press
eBook ISBN:
Hardcover ISBN:


@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY