Description

Since biological tissues are unstable in an oxygen atmosphere, a great deal of effort is expended by organisms to metabolically limit or repair oxidative tissue damage. This volume of Methods in Enzymology and its companion Volume 233 present methods developed to investigate the roles of oxygen radicals and antioxidants in disease. It includes techniques and assays of the activity of biothiols in soluble systems, their interaction with lipids and proteins, and the various new thiol enzymes, such as thioltransferases, being elucidated. Antioxidant reactions and gene regulation are also included.

Readership

Biochemists, pharmacologists, physiologists, and cell biologists.

Table of Contents

Oxidative Damage to DNA and DNA Repair: M. Dizdaroglu, Chemical Determination of Oxidative DNA Damage by Gas Chromatography-Mass Spectrometry. M.K. Shigenaga, E.N. Aboujaoude, Q. Chen, and B.N. Ames, Assays of the Oxidative DNA Damage Biomarker 8-Oxo-2-deoxyguanosine and 8-Oxoguanine in Nuclear DNA and Biological Fluids by High-Performance Liquid Chromatography with Electrochemical Detection. K.K. Hamilton, K. Lee, and P.W. Doetsch, Detection and Characterization of Eukaryotic Enzymes That Recognize Oxidative DNA Damage. W.A. Schulz, M.S.W. Obendorf, and H. Sies, Localization of Strand Breaks in Plasmid DNA Treated with Reactive Oxygen Species. Y. Luo, E.S. Henle, R. Chatopadhyaya, R. Jin, and S. Linn, Detecting DNA Damage Caused by Iron and Hydrogen Peroxide. P.K. Wong and R.A. Floyd, Photochemical Synthesis of 8-Hydroxyguanine Nucleosides. M.J. Burkitt, Copper (DNA Adducts). J. Cadet. J.-L. Ravanat, G.W. Buchko, H.C. Yeo, and B.N. Ames, Singlet Oxygen DNA Damage: Chromatographic and Mass Spectrometric Analysis of Damage Products. C. Baumstark-Khan, Alkaline Elution versus Fluorescence Analysis of DNA Unwinding. L. Augeri, K.K. Hamilton, A.M. Martin, P. Yohannes, and P.W. Doetsch, Purification and Properties of Yeast Redoxyendonuclease. C.F.M. Menck, Shuttle Vector between Prokaryotes and Eukaryotes for Assaying Singlet Oxygen-Induced DNA Damage and Mutagenicity. B. Epe and J. Hegler, Oxidative DNA Damage: Endonuclease Fingerprinting. Assay of Stress Genes/Proteins, Effects of Reactive Oxygen Species and Antioxidants on Cell Regulatory Processes: W. Drige, S. Mihm, M. Bockstette, and S. Roth, Effect of Reactive Oxygen Intermediates and An

Details

No. of pages:
704
Language:
English
Copyright:
© 1994
Published:
Imprint:
Academic Press
Electronic ISBN:
9780080883472
Print ISBN:
9780121821357

About the serial-volume-editor

Helmut Sies

Helmut Sies is an Honorary Member of the American Society for Biochemistry and Molecular Biology. He received an Honorary Ph.D. from the University of Buenos Aires, Argentina in 1996. Dr. Sies is a member of the Northrhine-Westphalian Academy of Sciences, Germany, and a Corresponding Member of both the Academy of Sciences of Heidelberg, Germany, and the Academy of Medicine, Buenos Aires, Argentina. He has received many awards and prizes, including the FEBS Anniversary Prize awarded by the Federation of European Biochemical Societies, 1978; the Distinguished Foreign Scholar award, MASUA, 1985; the Silver Medal, Karolinska Institute, Stockholm, 1986; the Ernst Jung Preis fur Medizin, 1988; the Claudius-Galenus-Preis, 1990; and the ISFE-Preis, 1994. Dr. Sies sereves on the editorial board and advisory committee for twelve journals, has edited numerous books, and has published more than 400 original articles and chapters. He received his M.D. at the University of Munich in 1967 and currently serves as Full Professor and Chairman of the Department of Physiological Chemistry at the University of Düsseldorf.

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY