Since biological tissues are unstable in an oxygen atmosphere, a great deal of effort is expended by organisms to metabolically limit or repair oxidative tissue damage. This volume of Methods in Enzymology and its companion Volume 233 present methods developed to investigate the roles of oxygen radicals and antioxidants in disease. It includes techniques and assays of the activity of biothiols in soluble systems, their interaction with lipids and proteins, and the various new thiol enzymes, such as thioltransferases, being elucidated. Antioxidant reactions and gene regulation are also included.
Biochemists, pharmacologists, physiologists, and cell biologists.
Oxidative Damage to DNA and DNA Repair:
M. Dizdaroglu, Chemical Determination of Oxidative DNA Damage by Gas Chromatography-Mass Spectrometry.
M.K. Shigenaga, E.N. Aboujaoude, Q. Chen, and B.N. Ames, Assays of the Oxidative DNA Damage Biomarker 8-Oxo-2-deoxyguanosine and 8-Oxoguanine in Nuclear DNA and Biological Fluids by High-Performance Liquid Chromatography with Electrochemical Detection.
K.K. Hamilton, K. Lee, and P.W. Doetsch, Detection and Characterization of Eukaryotic Enzymes That Recognize Oxidative DNA Damage.
W.A. Schulz, M.S.W. Obendorf, and H. Sies, Localization of Strand Breaks in Plasmid DNA Treated with Reactive Oxygen Species.
Y. Luo, E.S. Henle, R. Chatopadhyaya, R. Jin, and S. Linn, Detecting DNA Damage Caused by Iron and Hydrogen Peroxide.
P.K. Wong and R.A. Floyd, Photochemical Synthesis of 8-Hydroxyguanine Nucleosides.
M.J. Burkitt, Copper (DNA Adducts).
J. Cadet. J.-L. Ravanat, G.W. Buchko, H.C. Yeo, and B.N. Ames, Singlet Oxygen DNA Damage: Chromatographic and Mass Spectrometric Analysis of Damage Products.
C. Baumstark-Khan, Alkaline Elution versus Fluorescence Analysis of DNA Unwinding.
L. Augeri, K.K. Hamilton, A.M. Martin, P. Yohannes, and P.W. Doetsch, Purification and Properties of Yeast Redoxyendonuclease.
C.F.M. Menck, Shuttle Vector between Prokaryotes and Eukaryotes for Assaying Singlet Oxygen-Induced DNA Damage and Mutagenicity.
B. Epe and J. Hegler, Oxidative DNA Damage: Endonuclease Fingerprinting.
Assay of Stress Genes/Proteins, Effects of Reactive Oxygen Species and Antioxidants on Cell Regulatory Processes:
W. Drige, S. Mihm, M. Bockstette, and S. Roth, Effect of Reactive Oxygen Intermediates and An
- No. of pages:
- © Academic Press 1994
- 12th August 1994
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
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