Description

Since biological tissues are unstable in an oxygen atmosphere, a great deal of effort is expended by organisms to metabolically limit or repair oxidative tissue damage. This volume of Methods in Enzymology and its companion Volume 234 present methods developed to investigate the roles of oxygen radicals and antioxidants in disease.

Key Features

@introbul:Key Features @bul:* Generation, detection, and characterization of oxygen radicals, chemistry, biochemistry, and intermediate states of reductio* Isolation, characterization, and assay of enzymes or substrates involved in formation or removal of oxygen radical * Methods for assessing molecular, cell, and tissue damage; assays and repair of oxidative damage

Readership

Biochemists, pharmacologists, physiologists, and cell biologists.

Table of Contents

Generation, Detection, and Characterization of Oxygen Radicals in Chemistry, Biochemistry, and Intermediate States of Reduction: C. von Sonntag and H.-P. Schuchmann, Pulse Radiolysis. M. Saran and W. Bors, Pulse Radiolysis for Investigation of Nitric Oxide-Related Reactions. M. D'Aquino, C. Bullion, M. Chopra, D. Devi, S. Devi, C. Dunster, G. James, E. Komuro, E. Niki, F. Raza, F. Robertson, J. Sharma, and R. Willson, Sulfhydryl Free Radical Formation by Sonolysis, by Radiolysis, and Thermally: Vitamin A, Curcumin, Muconic Acid, and Related Conjugated Olefins and References. C. von Sonntag and H.-P. Schuchmann, Suppression of Hydroxyl Radical Reactions in Biological Systems: Considerations Based on Competition Kinetics. O.I Aruoma, Deoxyribose Assay for Detecting Hydroxyl Radicals. H. Kaur and B. Halliwell, Detection of Hydroxyl Radicals by Aromatic Hydroxylation. P.J. Evans and B. Halliwell, Measurement of Iron and Copper in Biological Systems: Blemycin and Copper-Phenanthroline Assays. L.M. Weiner, Oxygen Radicals Generation and DNA Scission by Anticancer and Synthetic Quinones. G.M. Rosen, S. Pou, B.E. Britigan, and M.S. Cohen, Spin Trapping of Hydroxyl Radicals in Biological Systems. R.P. Mason and K.T. Knecht, In Vivo Detection of Radical Adducts by Electron Spin Resonance. E. Albano, A. Tomasi, and M. Ingelman-Sundberg, Spin Trapping of Alcohol-Derived Radicals in Microsomes and Reconstituted Systemsby Electron Spin Resonance. M. Tsuchiya, M. Suematsu, and H. Suzuki, In Vivo Visualization of Oxygen Radical-Dependent Photoemission. J. Fuchs, N. Groth, T. Herrling, and L. Packer, In Vivo Electron Paramagnetic Resonance Imaging of Skin. A.

Details

No. of pages:
711
Language:
English
Copyright:
© 1994
Published:
Imprint:
Academic Press
Electronic ISBN:
9780080883465
Print ISBN:
9780121821340

About the serial-volume-editor

Reviews

@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY