For over fifty years the Methods in Enzymology series has been the critically acclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume features articles on the topic of oxygen biology and hypoxia.


Biochemists and researchers in related life science disciplines.

Table of Contents

Section I. Hypoxia-inducible factor Chapter 1 HIF PAS domains: structure and function Thomas H. Scheuermann, Jinsong Yang, Lei Zhang, Kevin H. Gardner, and Richard K. Bruick Chapter 2 Structure-function analysis of HIF Lorenz Poellinger Chaper 3 HIF Prolyl Hydroxylase: Purification and Assays of PHD2 Kirsty S. Hewitson, Christopher J. Schofield, Peter J. Ratcliffe Chapter 4 Determination and modulation of prolyl-4-hydroxylase domain (PHD) oxygen sensor activity Renato Wirthner, Kuppusamy Balamurugan, Daniel P. Stiehl, Sandra Barth, Patrick Spielmann, Felix Oehme, Ingo Flamme, Dörthe M. Katschinski, Roland H. Wenger and Gieri Camenisch Chapter 5 Characterisation of ankyrin-repeat containing proteins as substrates of the asparaginyl hydroxylase Factor Inhibiting HIF (FIH-1). Sarah Linke, Rachel J. Hampton-Smith, Daniel J. Peet Chapter 6 Transgenic Models to Understand HIF Function Andrew Doedens and Randall S. Johnson Chapter 7 The Silencing Approach of the Hypoxia Signalling Pathway" Edurne Berra and Jacques Pouyssegur Chapter 8 Cellular and developmental adaptations to hypoxia; a Drosophila perspective Nuria Magdalena Romero, Andrés Dekanty, Pablo Wappner Section II. Erythropoietin Chapter 9 Constitutively overexpressed erythropoietin reduces infarct size in a mouse model of permanent coronary artery ligation Giovanni G. Camici, Thomas Stallmach, Matthias Hermann, Rutger Hassink, Peter Doevendans, Beat Grenacher, Alain Hirschy, Johannes Vogel, Thomas F. Lüscher, Fr


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© 2007
Academic Press
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