Description

General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Oxidative Damage to Lipids, Proteins, and Nucleic Acids * Antioxidant Assays in Cells, Body Fluids, and Tissues * Oxidant and Redox Sensitive Steps in Signal Transduction and Gene Expression * Noninvasive Methods

Readership

AUDIENCE: Researchers in biochemistry, pharmacology, physiology, cell biology, molecular biology, and biomedicine.

Table of Contents

Oxidative Damage to Lipids, Proteins and Nucleic Acids: Lipids: J.D. Morrow and L.J. Roberts II,Mass Spectrometric Quantification of F2-Isoprostanes in Biological Fluids and Tissues as a Measure of Oxidant Stress. J. Nourooz-Zadeh, Improved GC-MS Assay for the Measurement of Plasma Isoprostanes. G.T. Shwaery, H. Mowri, J.F. Keaney, Jr., and B. Frei, Preparation of Lipid Hydroperoxide-Free LDL. W. Korytowski, PG. Geiger, and A.W. Girotti, Lipid Hydroperoxide Analysis by High-Performance Liquid Chromatography with Mercury Cathode Electrochemical Detection. A.M. Pastorino, A. Zamburlini, L. Zennaro, M. Maiorino, and F. Ursini, Measurement of Lipid Hydroperoxides in Human Plasma and Lipoproteins by Kinetic Analysis of Photon Emission. G.J. Handelman, HPLC Analysis of Cholesterol Linoleate Hydroperoxide in Oxidized LDL, with Calibration by Conjugated Diene Internal Standard. G.T. Shwaery, J.M. Samii, B. Frei, and J.F. Keaney, Jr., Determination of Phospholipid Oxidation in Cultured Cells. J. Nourooz-Zadeh, Ferrous Ion Oxidation in the Presence of Xylenol Orange for the Detection of Lipid Hydroperoxides in Plasma. T.R. Dugas and D.F. Church, Purification and Characterization of Phospholipid for Use in Lipid Oxidation Studies. H.C. Yeo, J.K. Liu, H.J. Helbock, K.B. Beckman, and B.N. Ames, Malondialdehyde Assay in Biological Fluids by Gas Chromatography-Mass Spectrometry. P.A. Glascott, Jr. and J. Farber, Assessment of the Physiological Interaction Between Vitamin E and Vitamin C. Proteins and Nucleic Acids: S.L. Hazen, F.F. Hsu, J.P. Gaut, J.R. Crowley, and J.W. Heinecke, Modification of Proteins and Lipids by Myeloperoxidase.

Details

No. of pages:
530
Language:
English
Copyright:
© 1999
Published:
Imprint:
Academic Press
eBook ISBN:
9780080884110
Print ISBN:
9780121822019

About the serial-volume-editor

Helmut Sies

Helmut Sies is an Honorary Member of the American Society for Biochemistry and Molecular Biology. He received an Honorary Ph.D. from the University of Buenos Aires, Argentina in 1996. Dr. Sies is a member of the Northrhine-Westphalian Academy of Sciences, Germany, and a Corresponding Member of both the Academy of Sciences of Heidelberg, Germany, and the Academy of Medicine, Buenos Aires, Argentina. He has received many awards and prizes, including the FEBS Anniversary Prize awarded by the Federation of European Biochemical Societies, 1978; the Distinguished Foreign Scholar award, MASUA, 1985; the Silver Medal, Karolinska Institute, Stockholm, 1986; the Ernst Jung Preis fur Medizin, 1988; the Claudius-Galenus-Preis, 1990; and the ISFE-Preis, 1994. Dr. Sies sereves on the editorial board and advisory committee for twelve journals, has edited numerous books, and has published more than 400 original articles and chapters. He received his M.D. at the University of Munich in 1967 and currently serves as Full Professor and Chairman of the Department of Physiological Chemistry at the University of Düsseldorf.

Affiliations and Expertise

Institut Fur Physiologische Chemie, Germany

Reviews

@from:Praise for the Volume @qu:"This volume is highly recommended for anyone with an interest in studies of NO. Since applications in so many different areas are covered and since the roles of NO are so varied and its presence ubiquitous, this volume should be of general usefulness for investigators in many different fields." @source:--Gene A. Homandberg, PhD, Rush Medical College of Rush University for DOODY'S ELECTRONC JOURNAL @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists."