Organized Multienzyme Systems: Catalytic Properties

Organized Multienzyme Systems: Catalytic Properties

1st Edition - January 28, 1985

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  • Editor: G. Rickey Welch
  • eBook ISBN: 9780323149105

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Organized Multienzyme Systems: Catalytic Properties describes the kinetic and catalytic properties of organized enzyme systems. This book is composed of nine chapters that specifically cover both immobilized and naturally occurring systems. The first two chapters examine the nature and function of enzyme organization in the mitochondrion, as well as the structural/functional coupling of the components in energy-transducing membrane systems. These topics are followed by discussions on ""dynamic compartmentation"" in soluble multienzyme systems; the allosteric enzyme systems; and allosterism in reversibly adsorptive enzyme systems. Other chapters explore model studies with specific immobilized multienzyme sequences, as regards the analysis of microenvironmental effects, and the mathematical exposition on the kinetic analysis of multienzyme systems in homogeneous solution. The last chapters present some theoretical and experimental studies on the behavior of immobilized systems. These chapters also provide a speculative integrative view of the kind of functional coherence that may be operative in organized states in vivo. This book is of great value to cell biologists, biochemists, and enzyme scientists and researchers.

Table of Contents

  • Contributors


    Chapter 1. Organization of Proteins within the Mitochondrion

    I. Biological Organization

    II. The Mitochondrion

    III. Concluding Remarks


    Chapter 2. Catalytic Facilitation and Membrane Bioenergetics

    I. Introduction

    II. Structural Dynamics of Energy-Coupling Membranes

    III. Protonmotive Functions of Electron Transport and ATP Synthase Complexes

    IV. The Protonmotive Force as an Intermediate in Electron-Transport Phosphorylation?

    V. The Effectiveness of Reconstituted Systems in Catalyzing ATP Synthesis

    VI. How Then Might Energy Coupling Proceed?

    VII. Protoneural Proteins?

    VIII. Mechanisms of Uncoupling

    IX. Control Theoretical View of Energy Coupling

    X. On the "Organization" of Energy-Coupling Membranes and Other Organized Multienzyme Systems


    Chapter 3. Dynamic Compartmentation in Soluble Multienzyme Systems

    I. Introduction

    II. Macrocompartments versus Microcompartments

    III. Static versus Dynamic Compartments

    IV. Evidence for Structural Enzyme Organization in Glycolysis

    V. Models of Dynamic Compartmentation in Soluble Enzyme Systems

    VI. Summary and Perspectives


    Chapter 4. Organized Polymeric Enzyme Systems: Catalytic Properties

    I. Introduction

    II. Protein Flexibility as the Prima Ratio of Allosteric Enzyme Behavior

    III. Phenomenology of Multiple Ligand Binding to Enzymes

    IV. The Concerted and the Induced-Fit Models of Ligand Binding

    V. Phenomenological Kinetics of Allosteric Enzymes

    VI. Thermodynamics of Subunit Interactions and the Principles of Structural Kinetics

    VII. Structural Formulation of Steady-State Enzyme Reaction Rates and Equilibrium Ligand Binding Isotherms

    VIII. The Antagonism between Substrate Binding and Enzyme Reaction Rate

    IX. The Evolution of Enzyme Flexibility and Cooperativity

    X. Conclusions


    Chapter 5. Control of Enzyme Activity in Reversibly Adsorptive Enzyme Systems

    I. Introduction

    II. Experimental Data Supporting the Concept That Enzymes May Be Adsorbed to Subcellular Structures under Physiological Conditions

    III. Types of Reversibly Adsorptive Enzyme Systems

    IV. Characteristics of Reversibly Adsorptive Enzyme Systems

    V. Changes in Catalytic and Regulatory Properties of Enzymes by Their Adsorption

    VI. Influence of Specific Ligands on Adsorption of Enzymes

    VII. Physiological Significance of Reversible Adsorption of Enzymes

    VIII. Conclusion


    Chapter 6. Models of Organized Multienzyme Systems: Use in Microenvironmental Characterization and in Practical Application

    I. Introduction

    II. Model Systems of Enzyme Sequences

    III. Role of Immobilized Enzyme Systems in the Interpretation of Microenvironmental Compartmentation in Vivo

    IV. Practical Applications of Immobilized Enzyme Sequences

    V. Concluding Remarks


    Chapter 7. Kinetic Analysis of Multienzyme Systems in Homogeneous Solution

    I. Introduction

    II. Unimolecular Unidirectional Closed Systems

    III. Unimolecular Bidirectional Closed Systems

    IV. Coupled Enzyme Systems—Polynomial Solutions

    V. Coupled Enzyme Systems—Approximations

    VI. Other Features of Coupled Enzyme Systems

    VII. Coupled Enzymes in the Pre-Steady State

    VIII. Parameter Estimation in Systems of Differential Equations

    IX. Conclusion


    Chapter 8. Theoretical and Experimental Studies on the Behavior of Immobilized Multienzyme Systems

    I. Introduction

    II. General Theoretical Treatment of Diffusion Reactions

    III. Experimental Studies

    IV. Conclusion and Perspectives


    Chapter 9. Long-Range Energy Continua and the Coordination of Multienzyme Sequences in Vivo

    I. Introduction

    II. Transduction and Conservation of Chemical Free Energy within Multienzyme Aggregates

    III. Enzymes: Biochemical Electrodes and Protodes

    IV. An Electrochemical Interpretation of Metabolism

    V. Long-Range Energy Continua and Metabolic Regulation

    VI. Concluding Remarks



Product details

  • No. of pages: 472
  • Language: English
  • Copyright: © Academic Press 1985
  • Published: January 28, 1985
  • Imprint: Academic Press
  • eBook ISBN: 9780323149105

About the Editor

G. Rickey Welch

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