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Organization and Practice in Tuberculosis Bacteriology - 1st Edition - ISBN: 9780407002968, 9781483162539

Organization and Practice in Tuberculosis Bacteriology

1st Edition

Authors: C. H. Collins J. M. Grange M. D. Yates
eBook ISBN: 9781483162539
Imprint: Butterworth-Heinemann
Published Date: 27th November 1985
Page Count: 136
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Organization and Practice in Tuberculosis Bacteriology focuses on the organization and technical methods of tuberculosis bacteriology. This book discusses the mycobacteria and mycobacterial disease and role and scope of the tuberculosis laboratory in human and veterinary medicine and epidemiology. The organization of tuberculosis laboratory services; collection, preservation and transport of specimens; and examination of direct smears are also elaborated. This text likewise covers the decontamination of specimens, inoculation of culture media, and identification of species and variants of tubercle bacilli. Other topics include the drug sensitivity tests, animal inoculation and assay tests; problems faced in the therapy of tuberculosis, and lipid chromatography. This publication is suitable for biologists, medical practitioners, and clinicians concerned with tuberculosis bacteriology.

Table of Contents

1 Introduction: Mycobacteria and Mycobacterial Disease


Other Mycobacterioses in Man

2 The Role and Scope of the Tuberculosis Laboratory in Human and Veterinary Medicine and Epidemiology

3 The Organization of Tuberculosis Laboratory Services

Grades of Laboratories

Design of the Tuberculosis Laboratory


Training and Motivation of Staff

Proficiency Testing

Prevention of Laboratory-Acquired Tuberculosis

4 Collection, Preservation and Transport of Specimens



Aspirated Fluids and Pus


Milk Samples

Water Samples

Identifying Specimens

Preservation of Specimens


5 Examination of Direct Smears

Preparation of Specimens


Quantitative Microscopy

Precautions against False Positives

Positive Smears - Negative Cultures

Acid- and Alcohol-Fast Bacilli

Storage and Disposal of Slides

6 Cultural Methods

Decontamination of Specimens

Culture Media

Inoculation of Culture Media


Examination of Cultures

Radiometrie Method for TB Culture

7 Taxonomic Principles - The Basis of Identification

8 Identification of Species and Variants of Tubercle Bacilli



Identification of Variants of M. Tuberculosis and BCG

Variants and Species of Tubercle Bacilli

9 Identification of 'Other Mycobacteria'


Identification Methods

Cultural and Biochemical Tests

Lipid Chromatography

Additional Biochemical Tests

Additional Reference Techniques

Species of Medical and Veterinary Interest

Mycobacterium Avium

Mycobacterium Avium-Intracellulare-Scrofulaceum '(MAIS)'

Mycobacterium Chelonei

Mycobacterium Flavescens

Mycobacterium Fortuitum

Mycobacterium Gastri

Mycobacterium Gordonae

Mycobacterium Haemophilum

Mycobacterium Intracellulare

Mycobacterium Kansasii

Mycobacterium Malmoense

Mycobacterium Marinum

Mycobacterium Nonchromogenicum

Mycobacterium Paratuberculosis

Mycobacterium Phlei

Mycobacterium Scrofulaceum

Mycobacterium Simiae

Mycobacterium Smegmatis

Mycobacterium Szulgai

Mycobacterium Terrae

Mycobacterium Triviale

Mycobacterium Ulcerans

Mycobacterium Xenopi

Species of Limited Interest in Human and Veterinary Medicine

10 Drug Sensitivity Tests

The theory of Drug Resistance and Treatment Regimens

Principles of Treatment Regimens

Development of Modern Treatment Regimens

Problems Faced in the Therapy of Tuberculosis

The Treatment of Other Mycobacterial Infections

The Role of Sensitivity Testing

Methods for Sensitivity Tests

11 Animal Inoculation and Assay Tests

Animal Inoculation Tests

Assay of Antimicrobial Agents in Serum and their Detection in Urine

12 Leprosy

Technical Procedures

The Skin

The Nose


Appendix Stains, Culture Media and Other Materials




No. of pages:
© Butterworth-Heinemann 1985
27th November 1985
eBook ISBN:

About the Authors

C. H. Collins

J. M. Grange

M. D. Yates

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