Organization and Practice in Tuberculosis Bacteriology

Organization and Practice in Tuberculosis Bacteriology

1st Edition - November 27, 1985

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  • Authors: C. H. Collins, J. M. Grange, M. D. Yates
  • eBook ISBN: 9781483162539

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Organization and Practice in Tuberculosis Bacteriology focuses on the organization and technical methods of tuberculosis bacteriology. This book discusses the mycobacteria and mycobacterial disease and role and scope of the tuberculosis laboratory in human and veterinary medicine and epidemiology. The organization of tuberculosis laboratory services; collection, preservation and transport of specimens; and examination of direct smears are also elaborated. This text likewise covers the decontamination of specimens, inoculation of culture media, and identification of species and variants of tubercle bacilli. Other topics include the drug sensitivity tests, animal inoculation and assay tests; problems faced in the therapy of tuberculosis, and lipid chromatography. This publication is suitable for biologists, medical practitioners, and clinicians concerned with tuberculosis bacteriology.

Table of Contents

  • 1 Introduction: Mycobacteria and Mycobacterial Disease


    Other Mycobacterioses in Man

    2 The Role and Scope of the Tuberculosis Laboratory in Human and Veterinary Medicine and Epidemiology

    3 The Organization of Tuberculosis Laboratory Services

    Grades of Laboratories

    Design of the Tuberculosis Laboratory


    Training and Motivation of Staff

    Proficiency Testing

    Prevention of Laboratory-Acquired Tuberculosis

    4 Collection, Preservation and Transport of Specimens



    Aspirated Fluids and Pus


    Milk Samples

    Water Samples

    Identifying Specimens

    Preservation of Specimens


    5 Examination of Direct Smears

    Preparation of Specimens


    Quantitative Microscopy

    Precautions against False Positives

    Positive Smears - Negative Cultures

    Acid- and Alcohol-Fast Bacilli

    Storage and Disposal of Slides

    6 Cultural Methods

    Decontamination of Specimens

    Culture Media

    Inoculation of Culture Media


    Examination of Cultures

    Radiometrie Method for TB Culture

    7 Taxonomic Principles - The Basis of Identification

    8 Identification of Species and Variants of Tubercle Bacilli



    Identification of Variants of M. Tuberculosis and BCG

    Variants and Species of Tubercle Bacilli

    9 Identification of 'Other Mycobacteria'


    Identification Methods

    Cultural and Biochemical Tests

    Lipid Chromatography

    Additional Biochemical Tests

    Additional Reference Techniques

    Species of Medical and Veterinary Interest

    Mycobacterium Avium

    Mycobacterium Avium-Intracellulare-Scrofulaceum '(MAIS)'

    Mycobacterium Chelonei

    Mycobacterium Flavescens

    Mycobacterium Fortuitum

    Mycobacterium Gastri

    Mycobacterium Gordonae

    Mycobacterium Haemophilum

    Mycobacterium Intracellulare

    Mycobacterium Kansasii

    Mycobacterium Malmoense

    Mycobacterium Marinum

    Mycobacterium Nonchromogenicum

    Mycobacterium Paratuberculosis

    Mycobacterium Phlei

    Mycobacterium Scrofulaceum

    Mycobacterium Simiae

    Mycobacterium Smegmatis

    Mycobacterium Szulgai

    Mycobacterium Terrae

    Mycobacterium Triviale

    Mycobacterium Ulcerans

    Mycobacterium Xenopi

    Species of Limited Interest in Human and Veterinary Medicine

    10 Drug Sensitivity Tests

    The theory of Drug Resistance and Treatment Regimens

    Principles of Treatment Regimens

    Development of Modern Treatment Regimens

    Problems Faced in the Therapy of Tuberculosis

    The Treatment of Other Mycobacterial Infections

    The Role of Sensitivity Testing

    Methods for Sensitivity Tests

    11 Animal Inoculation and Assay Tests

    Animal Inoculation Tests

    Assay of Antimicrobial Agents in Serum and their Detection in Urine

    12 Leprosy

    Technical Procedures

    The Skin

    The Nose


    Appendix Stains, Culture Media and Other Materials



Product details

  • No. of pages: 136
  • Language: English
  • Copyright: © Butterworth-Heinemann 1985
  • Published: November 27, 1985
  • Imprint: Butterworth-Heinemann
  • eBook ISBN: 9781483162539

About the Authors

C. H. Collins

J. M. Grange

M. D. Yates

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