Organic Trace Analysis by Liquid Chromatography

Organic Trace Analysis by Liquid Chromatography

1st Edition - October 28, 1981

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  • Author: James Lawrence
  • eBook ISBN: 9780323157025

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Organic Trace Analysis by Liquid Chromatography focuses on high-performance liquid chromatography in the field of formulations analysis, which includes quality control of pharmaceutical preparations, pesticide formulations, cosmetics, and food colors. This book explores the application of liquid chromatography to trace analysis. Organized into 10 chapters, this book starts with an overview of the special requirements of liquid chromatography for trace analysis. This text then compares the approach to gas chromatography and formulations analysis, noting the significant difference. Other chapters consider the liquid chromatography equipment and examine the best types and conditions of instrumentation suitable for trace analysis. This book discusses as well the chromatography theory and includes descriptive accounts of the principles of the different forms of chromatography. The final chapter describes the typical approaches that are used for trace analysis. This book is a valuable resource for analysts engaged in the determination of trace organics in many various substrates.

Table of Contents

  • Preface

    1 General Considerations in Developing a Trace Analytical Technique Employing Liquid Chromatography

    I. Liquid Chromatography versus Gas Chromatography

    II. Residue Analysis versus Formulations Analysis

    III. Pump and Injector Requirements

    IV. Detectors

    V. Chromatography

    VI. Extraction and Cleanup

    VII. Minimum Detectable Levels

    VIII. Matrix Effects

    IX. Use of Derivatization

    X. Microprocessors and Automation


    2 Pumping Systems

    I. Introduction

    II. Constant Volume Pumps

    III. Constant Pressure Pumps

    IV. Gradient Elution

    V. Comparison of Pumping Systems

    3 Sampling Technique and Injection Ports

    I. Effect of Sample Solution on Peak Shape

    II. Requirements for Trace Analysis

    III. Types of Injection Ports


    4 Chromatography Columns and Packing Materials

    I. Requirements for Trace Analysis

    II. Pellicular versus Microparticulate Porous Packings

    III. Commercial Prepacked Columns

    IV. Column Packing Techniques

    V. Stationary-Phase Materials


    5 Detectors

    I. Introduction

    II. General Detector Requirements

    III. Photometric Detectors

    IV. Electrochemical Detectors

    V. Solute Transport Detectors

    VI. Refractive Index Detectors

    VII. Mass Spectrometer

    VIII. Other Detectors


    6 Chromatography Theory

    I. Fundamental Principles

    II. Adsorption Chromatography

    III. Partition Chromatography

    IV. Macroreticular Resins

    V. Ion-Pair Chromatography

    VI. Ion-Exchange Chromatography

    VII. Ion Chromatography

    VIII. Gel Permeation Chromatography

    IX. Ligand-Exchange Chromatography

    X. Charge-Transfer Chromatography


    7 Chemical Derivatization

    I. Introduction

    II. UV-Absorbance Derivatization

    III. Fluorescence Derivatization

    IV. Derivatization for Other Detection Modes

    V. Postcolumn Reactions


    8 Sample Extraction and Cleanup

    I. Considerations

    II. Sample Extraction

    III. Sample Cleanup

    IV. Column Chromatography

    V. Thin-Layer Chromatography

    VI. Distillation

    VII. Low-Temperature Precipitation


    9 Approach to Method Development and Routine Analysis

    I. Choosing the Best Chromatography System

    II. Analysis Time and Interfering Peaks

    III. Qualitative and Quantitative Analysis and Confirmation

    IV. Automation

    V. Integration of LC with Other Analytical Techniques

    VI. LC as a Cleanup Technique


    10 Applications

    I. Introduction

    II. Clinical (Biological Fluids and Tissues)

    III. Environmental

    IV. Foods



Product details

  • No. of pages: 300
  • Language: English
  • Copyright: © Academic Press 1981
  • Published: October 28, 1981
  • Imprint: Academic Press
  • eBook ISBN: 9780323157025

About the Author

James Lawrence

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