Optochemical Biology

Optochemical Biology

1st Edition - July 30, 2019

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  • Editor: Alexander Deiters
  • eBook ISBN: 9780128170298
  • Hardcover ISBN: 9780128170281

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Optochemical Biology, Volume 624, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters on a variety of topics, including Cell Lineage Tracing in Zebrafish Embryos with an Expanded Genetic Code, Optical Control of Tumor Induction in the Zebrafish, Optogenetic Control by Pulsed Illumination, Optimizing the Photocontrol of bZIP Coiled Coils with Azobenzene Crosslinkers: Role of the Crosslinking Site, Site-Directed RNA Editing in Vivo Can Be Triggered by the Light-Driven Assembly of an Artificial Riboprotein, In Situ Formation of an Azo Bridge on Proteins Controllable by Visible Light, and much more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Includes the latest information on optochemical biology


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. Optical control of protein activity and gene expression by photoactivation of caged cyclofen
    Fatima Hamouri, Weiting Zhang, Isabelle Aujard, Thomas Le Saux, Bertrand Ducos, Sophie Vriz, Ludovic Jullien and David Bensimon
    2. Reversible optogenetic control of protein function and localization
    Daniel Z. Wu, Rachel M. Lackner, Chanat Aonbangkhen, Michael A. Lampson and David M. Chenoweth
    3. Protocols for the generation of caged guideRNAs for light-triggered RNA-targeting with SNAP-ADARS
    Alfred Hanswillemenke and Thorsten Stafforst
    4. Combinatorial control of gene function with wavelength-selective caged Morpholinos
    Sankha Pattanayak, Luis Angel Vázquez-Maldonado, Alexander Deiters and James K. Chen
    5. Phosphodiester photo-tethers for the (multi-)cyclic conformational caging of oligonucleotides
    Patricia Müller, Patrick Seyfried, Anton Frühauf and Alexander Heckel
    6. Chemical modification of proteins with photocleavable groups
    Karthik Nadendla, Bhagyesh Sarode and Simon H. Friedman
    7. Intramolecular cross-linking of proteins with azobenzene-based cross-linkers
    Nobuo Yasuike, Huixin Lu, Peng Xia and G. Andrew Woolley
    8. Ruthenium-crosslinked hydrogels for rapid, visible-light protein release
    Teresa L. Rapp and Ivan J. Dmochowski
    9. Chromatically independent, two-color uncaging of glutamate and GABA with one- or two-photon excitation
    Stefan Passlick and Graham C.R. Ellis-Davies
    10. DIY optogenetics: Building, programming, and using the light plate apparatus
    Karl P. Gerhardt, Sebastian M. Castillo-Hair and Jeffrey J. Tabor
    11. Pulsatile illumination for photobiology and optogenetics
    Julia Dietler, Robert Stabel and Andreas Möglich
    12. Genetically encoding photoswitchable click amino acids for general optical control of conformation and function of proteins
    Christian Hoppmann and Lei Wang
    13. Light-activation of Cre recombinase in zebrafish embryos through genetic code expansion
    Wes Brown and Alexander Deiters

Product details

  • No. of pages: 298
  • Language: English
  • Copyright: © Academic Press 2019
  • Published: July 30, 2019
  • Imprint: Academic Press
  • eBook ISBN: 9780128170298
  • Hardcover ISBN: 9780128170281

About the Serial Volume Editor

Alexander Deiters

Dr. Alexander Deiters works at the University of Pittsburgh, USA

Affiliations and Expertise

University of Pittsburgh, USA

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