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Optical Techniques in Biological Research discusses the fundamentals and applications of light scattering, Raman scattering, Fourier transform infrared spectroscopy, nanosecond fluorescence spectroscopy, and circular dichroism. Electron microscopy is also included owing to some of its classical similarity to optical microscopy optical structural and resonance techniques for biological research. The chapters are aimed at a level such that only a general understanding of chemistry and biology is required. The objective is to present material in a way that allows the research worker to assess quickly the applicability, utility, and significance of the specific technique to his or her problem or field of interest. Together with Structural and Resonance Techniques in Biological Research, this book marks the introduction of a new series of volumes, Physical Techniques in Biology and Medicine, which is intended to replace a previous treatise, Physical Techniques in Biological Research.
List of Contributors
1. Light Scattering
List of Symbols
II. Elastic Light Scattering
III. Quasi-Elastic Light Scattering
IV. Laser Doppler Techniques
2. Raman Scattering
II. Theoretical Background
III. Experimental Procedures
IV. Nonresonance Raman Scattering
V. Resonance Raman Scattering
VI. Intact Organelles
VII. New Techniques
VIII. Concluding Remarks
3. Fourier Transform Infrared Spectroscopy
I. Infrared Spectroscopy
II. Biological Applications
III. Techniques of Measurement
IV. Instrumental Considerations
V. Computational Problems in Fourier Transform Spectroscopy
VI. Summary and Conclusion
4. Nanosecond Fluorescence Spectroscopy
II. Measurement of Nanosecond Emission
III. Analysis of Nanosecond Fluorescence Data
IV. Analysis of Nanosecond Fluorescence Anisotropy
5. Circular Dichroism
III. Application to Biological Problems
IV. Scattering Particles
V. Protein Molecules
VI. Extensions of Circular Dichroism Techniques
6. Electron Microscopy
II. Formation of Electron Images
III. The Instrument
IV. Specimen Preparation
V. Special Applications
VI. Image Processing
VII. Other Short-Wavelength Microscopes
Appendix I: Theory of Imaging
- No. of pages:
- © Academic Press 1984
- 14th December 1984
- Academic Press
- eBook ISBN:
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