Optical Techniques in Biological Research

Optical Techniques in Biological Research

1st Edition - December 14, 1984

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  • Editor: Denis Rousseau
  • eBook ISBN: 9780323150217

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Optical Techniques in Biological Research discusses the fundamentals and applications of light scattering, Raman scattering, Fourier transform infrared spectroscopy, nanosecond fluorescence spectroscopy, and circular dichroism. Electron microscopy is also included owing to some of its classical similarity to optical microscopy optical structural and resonance techniques for biological research. The chapters are aimed at a level such that only a general understanding of chemistry and biology is required. The objective is to present material in a way that allows the research worker to assess quickly the applicability, utility, and significance of the specific technique to his or her problem or field of interest. Together with Structural and Resonance Techniques in Biological Research, this book marks the introduction of a new series of volumes, Physical Techniques in Biology and Medicine, which is intended to replace a previous treatise, Physical Techniques in Biological Research.

Table of Contents

  • List of Contributors

    Series Introduction


    1. Light Scattering

    List of Symbols

    I. Introduction

    II. Elastic Light Scattering

    III. Quasi-Elastic Light Scattering

    IV. Laser Doppler Techniques


    2. Raman Scattering

    I. Introduction

    II. Theoretical Background

    III. Experimental Procedures

    IV. Nonresonance Raman Scattering

    V. Resonance Raman Scattering

    VI. Intact Organelles

    VII. New Techniques

    VIII. Concluding Remarks


    3. Fourier Transform Infrared Spectroscopy

    I. Infrared Spectroscopy

    II. Biological Applications

    III. Techniques of Measurement

    IV. Instrumental Considerations

    V. Computational Problems in Fourier Transform Spectroscopy

    VI. Summary and Conclusion


    General References

    4. Nanosecond Fluorescence Spectroscopy

    I. Introduction

    II. Measurement of Nanosecond Emission

    III. Analysis of Nanosecond Fluorescence Data

    IV. Analysis of Nanosecond Fluorescence Anisotropy

    V. Applications


    5. Circular Dichroism

    I. Introduction

    II. Methods

    III. Application to Biological Problems

    IV. Scattering Particles

    V. Protein Molecules

    VI. Extensions of Circular Dichroism Techniques

    VII. Theory

    VIII. Instrumentation

    IX. Summary


    6. Electron Microscopy

    I. Introduction

    II. Formation of Electron Images

    III. The Instrument

    IV. Specimen Preparation

    V. Special Applications

    VI. Image Processing

    VII. Other Short-Wavelength Microscopes

    VIII. Epilogue

    Appendix I: Theory of Imaging



Product details

  • No. of pages: 442
  • Language: English
  • Copyright: © Academic Press 1984
  • Published: December 14, 1984
  • Imprint: Academic Press
  • eBook ISBN: 9780323150217

About the Editor

Denis Rousseau

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