General Description of the Volume: Oncogenes have become a central focus of cancer research. All involved in this discipline will find this volume an invaluable aid in developing protocols needed for their research. General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Cell culture * Molecular cloning * Gene function * Protein-protein interactions * Protein-DNA interactions


Biochemists, oncologists, geneticists, molecular biologists, microbiologists, biomedical researchers, and cell biologists.

Table of Contents

Cells: W.C. Weinberg, S.H. Yuspa, A.A. Dlugosz, A.B. Glick, and T. Tennenbaum, Isolation and Utilization of Epidermal Keratinocytes for Oncogene Research. J. Ray, H.K. Raymon, and F.H. Gage, Generation and Culturing of Precursor Cells and Neuroblasts from Embryonic and Adult Central Nervous System. H. Land, Transformation of Primary Rat Embryo Cells. H. Beug, P. Steinlein, P. Bartunek, and M.J. Hayman, Avian Hematopoietic Cell Culture: In Vitro Model Systems to Study Oncogenic Transformation of Hematopoietic Cells. P. Crisanti, B. Pessac, and G. Calothy, Avian Neuroretina Cells in Oncogene Studies. D. Boettiger and C. DiLullo, Myogenic and Chondrogenic Cells. W. Krek and J.A. DeCaprio, Cell Synchronication. T.D. Tlsty, A. Briot, and B. Poulose, Analysis of Cell Cycle Checkpoint Status in Mammalian Cells. A.M. Killary and R.E.K. Fournier, Microcell Fusion. M.P. Moyer, Tumor Cells Culture. Molecular Clones: T. Yamamoto and Y. Kadowaki, Superfamilies of Protooncogenes: Homology Cloning and Characterization of Related Members. M.A. Sells and J. Chernoff, PCR Cloning of Related Genes. T. Miki and S.A. Aaronson, Isolation of Oncogenes by Expression cDNA Cloning. J. Samarut, F. Flamant, and J.-M. Heard, Replication-Competent and -Defective Retrovirus Vectors for Oncogenic Studies. E.M. Blackwood and R.N. Eisenman, Identification of Protein-Protein Interactions by lambdagt11Expression Cloning. P.L. Bartel and S. Fields, Analyzing Protein-Protein Interactions Using Two-Hybrid System. G.G. Hicks, E.-g. Shi, J. Chen, M. Roshon, D. Williamson, C. Scherer, and H.E. Ruley


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© 1995
Academic Press
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About the serial-volume-editors


@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY