In this thematic volume of Progress in Molecular Biology and Translational Science, researchers reflect on recent developments and research surrounding G protein-coupled receptors. The chapters cover a large breadth of research, including GPCR role in stem cell function and pharmacology.

Authors explore in-depth research techniques and applications of GPCR usage, covering theory, laboratory approaches, and unique qualities that make GPCRs a crucial tool in microbiological and cancer research.

Key Features

Key features:

* Contributions from leading authorities
* Informs and updates on all the latest developments in the field


Researchers of G-protein receptors, molecular biology, and translational science

Table of Contents

  • Contributors
  • Preface
  • Chapter One. Approaches for Probing Allosteric Interactions at 7 Transmembrane Spanning Receptors
    • 1 Introduction
    • 2 General Considerations for Assays Designed to Identify and Characterize Allosteric Modulators
    • 3 General Workflow Used in Identifying and Characterizing Allosteric Modulators
    • 4 Data Analysis: General Features for Allosteric Interactions
    • 5 Kinetic Assays to Measure Allosteric Interactions at 7TMRs
    • 6 Label-Free Technology
    • 7 Endpoint Assays
    • 8 Radioligand Binding Assays for Allosteric Interactions
    • 9 Competitive or Not Competitive
    • 10 Conclusions and Future Directions
    • References
  • Chapter Two. Pharmacology of Metabotropic Glutamate Receptor Allosteric Modulators
    • Abbreviations
    • 1 Introduction
    • 2 Physiology and Pharmacology of mGlus
    • 3 Allosteric Modulation of mGlus
    • 4 Location of Allosteric Sites
    • 5 Therapeutic Indications for Allosteric Modulators
    • 6 Concluding Remarks
    • References
  • Chapter Three. Mining the Potential of Label-Free Biosensors for Seven-Transmembrane Receptor Drug Discovery
    • 1 Introduction
    • 2 Promises of Label-Free Screening
    • 3 Challenges of Label-Free Screening
    • 4 The Value Proposition of Label-Free to Drug Discovery
    • 5 Concluding Remarks
    • References
  • Chapter Four. G Protein-Coupled Receptors in Cancer
    • 1 General Introduction
    • 2 Biochemical Interactions
    • 3 Drug Design
    • References
  • Chapter Five. GPCRs in Stem Cell Function
    • Abbreviations
    • 1 Introduction
    • 2 Types of Stem Cells
    • 3 GPCRs in Embryonic Stem Cells
    • 4 GPCRs in Induced Pluripotent Stem Cells
    • 5 GPCRs in Adult Stem Cells
    • 6


No. of pages:
© 2013
Academic Press
Print ISBN:
Electronic ISBN:


Praise for the series:
"Full of interest not only for the molecular biologist-for whom the numerous references will be invaluable-but will also appeal to a much wider circle of biologists, and in fact to all those who are concerned with the living cell." --British Medical Journal