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Nucleosomes - 1st Edition - ISBN: 9780121820718, 9780080882871

Nucleosomes, Volume 170

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editor: Roger Kornberg
eBook ISBN: 9780080882871
Imprint: Academic Press
Published Date: 28th August 1989
Page Count: 683


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Table of Contents

Chromatin and Nucleosomes: Preparative Techniques: R.D. Kornberg, Preparation of Chromatin and Nucleosomes: General Considerations. P. Oudet, E. Weiss, and E. Regnier, Preparation of Simian Virus 40 Minichromsomes. A. Dean, D.S. Pederson, and R.T. Simpson, Isolation of Yeast Plasmid Chromatin. T.M. Herman, Affinity Isolation of Replicating Simian Virus 40 Replicating Minichromosomes. Chromatin and Nucleosomes: Analytical Techniques: H. Noll and M. Noll, Sucrose Gradient Techniques and Applications to Nucleosome Structure. S.Y. Huang and W.T. Garrard, Electrophoretic Analyses of Nucleosomes and Other Protein-DNA Complexes. J.M. Sogo and F. Thoma, Electron Microscopy of Chromatin. P. Oudet, P. Schultz, J.-C. Homo, and P. Colin, Electron Microscopy of Simian Virus 40 Minichromosomes. C.L.F. Woodcock, Immunoelectron Microscopy of Nucleosomes. J.-R. Daban and C.R. Cantor, Use of Fluorescent Probes to Study Nucleosomes. M. Bustin, Preparation and Application of Immunological Probes for Nucleosomes. S. Muller and M.H.V. Van Regenmortel, Use of Antihistone Antibodies with Nucleosomes. L.C. Lutter, Digestion of Nucleosomes with Deoxyribonucleases I and II. C. Wu, Analysis of Hypersensitive Sites in Chromatin. M.E. Cusick, P.M. Wassarman, and M.L. DePamphilis, Application of Nucleases to Visualizing Chromatin Organization at Replication Forks. M. Bellard, D. Dretzen, A. Giangrande, and P. Ramain, Nuclease Digestion of Transcriptionally Active Chromatin. J.M. Gottesfeld, Analysis of RNA Polymerase III Transcription in Vitro Using Chromatin and Cloned Gene Templates. I.L. Cartwright and S.C.R. Elgin, Nonenzymatic Cleavage of Chromatin. J.O. Thomas, Chemical Radiolabeling of Lysines that Interact Strongly with DNA in Chromatin. A.D. Mirzabekov, S.G. Bavykin, A.V. Belyavsky, V.L. Karpov, O.V. Preobrazhenskaya, V.V. Shich, and K.K. Ebralidse, Mapping DNA-Protein Interaction by Cross-Linking. S.A. Nedospasov, A.N. Shakhov, and G.P. Georgiev, Analysis of Nucleosome Positioning by Indirect End-Labeling and Molecular Cloning. D. Rhodes, R.S. Brown, and A. Klug, Crystallization of Nucleosome Core Particles. Histones: Preparation and Analysis: C. von Holt, W.F. Brandt, H.J. Greyling, G.G. Lindsey, J.T. Retief, J. de A. Rodrigues, S. Schwager, and B.T. Sewell, Isolation and Characterization of Histones. R.D. Cole, Purification and Analysis of H1 Histones. R.W. Lennox and L.H. Cohen, Analysis of Histone Subtypes and Their Modified Forms by Polyacrylamide Gel Electrophoresis. J.O. Thomas, Chemical Cross-Linking of Histones. Assembly and Reconstitution of Chromatin: D. Rhodes and R.A. Laskey, Assembly of Nucleosomes and Chromatin in Vitro. A. Stein, Reconstitution of Chromatin from Purified Components. A. Shimamura, B. Jessee, and A. Worcel, Assembly of Chromatin with Oocyte Extracts. L. Sealy, R.R. Burgess, M. Cotten, and R. Chalkley, Purification of Xenopus Egg Nucleoplasmin and Its Use in Chromatin Assembly in Vitro. B. Neubauer and W. H~adorz, Analysis of Nucleosome Positioning by in Vitro Reconstitution. Subject Index. Author Index.


The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.


Biochemists, molecular biologists, cell biologists, developmental biologists, and biophysicists.


No. of pages:
© Academic Press 1989
28th August 1989
Academic Press
eBook ISBN:


@from:Praise for the Volume @qu:"Its four sections cover a broad range of procedures designed to take you from cell to gel... The longest section, that on analytical techniques, starts with a review of the theory of sedimentation. Sucrose gradients are featured. Most of the other chapters here include preparatory methods, often for a specific organism or tissue." @source:--ANALYTICAL BIOCHEMISTRY @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY

About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editor

Roger Kornberg

Affiliations and Expertise

School of Medicine, Stanford University, California, U.S.A.