Description

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The volume covers nucleosomes, histones and chromatin and has chapters on dynamic mapping of histone-DNA interactions in nucleosomes by unzipping single molecules of DNA, digital DNase technology, and genome-wide analysis of chromatin transition.

Key Features

  • Contains quality chapters authored by leaders in the field
  • The volume covers nucleosomes, histones and chromatin
  • Has chapters on dynamic mapping of histone-DNA interactions in nucleosomes by unzipping single molecules of DNA, digital DNase technology, and genome-wide analysis of chromatin transition

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

18. TBC

  Anjana Rao


19. Quantitative affinity measurements for chromatin binding modules and their cognate marks-- pull-downs/ITC/FP/SPR

  Alex J. Ruthenburg


20. TBC

  Yang Shi


21. Modified Lysine Analogs for the study of chromatin modifying enzymes and nucleosome structure

  Kevin Shokat and Matthew D. Simon


22. Map the exact polydenylation site of mRNA transcripts

  Lars M. Steinmetz


23. Analysis of the mechanism of chromatin remodeling by

  Vasily M. Studitsky


24. TBC

  Michiel Vermeulen


25. Dynamic mapping of histone-DNA interactions in nucleosomes by unzipping single molecules of DNA

  Michelle D. Wang


26. Mechanisms of ATP-dependent chromatin remodelling revealed by high-resolution optical tweezers

  Yongli Zhang


27. Enzymatic analysis of the Tet proteins by TLC and mass spectrometry

  Yi Zhang


28. Investigating histone modifications using quantitative mass spectrometry

  Bing Zhu


29. Single-molecule studies of chromatin remodeling

  Xiaowei Zhuang


30. Genome-wide mapping of nucleosomes, chromatin modifications and chromatin-modifying enzymes

  Keji Zhao


31. Digital DNase technology

  John Stamatoyannopoulos


32. Genome-wide Analysis of Chromatin Transitions; Integration of Static and Dynamic Approaches

  Gordon Hager


33. Mapping the transcription machinery on the yeast genome

  Patrick Cramer


34. TBC

  Bradley R. Cairns

Details

No. of pages:
408
Language:
English
Copyright:
© 2012
Published:
Imprint:
Academic Press
Print ISBN:
9780123919380
Electronic ISBN:
9780123919397