Nucleosomes, Volume 170

1st Edition

Print ISBN: 9780121820718
eBook ISBN: 9780080882871
Imprint: Academic Press
Published Date: 28th August 1989
Page Count: 683


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The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.


Biochemists, molecular biologists, cell biologists, developmental biologists, and biophysicists.

Table of Contents

Chromatin and Nucleosomes: Preparative Techniques:

R.D. Kornberg, Preparation of Chromatin and Nucleosomes: General Considerations.

P. Oudet, E. Weiss, and E. Regnier, Preparation of Simian Virus 40 Minichromsomes.

A. Dean, D.S. Pederson, and R.T. Simpson, Isolation of Yeast Plasmid Chromatin.

T.M. Herman, Affinity Isolation of Replicating Simian Virus 40 Replicating Minichromosomes.

Chromatin and Nucleosomes: Analytical Techniques:

H. Noll and M. Noll, Sucrose Gradient Techniques and Applications to Nucleosome Structure.

S.Y. Huang and W.T. Garrard, Electrophoretic Analyses of Nucleosomes and Other Protein-DNA Complexes.

J.M. Sogo and F. Thoma, Electron Microscopy of Chromatin.

P. Oudet, P. Schultz, J.-C. Homo, and P. Colin, Electron Microscopy of Simian Virus 40 Minichromosomes.

C.L.F. Woodcock, Immunoelectron Microscopy of Nucleosomes.

J.-R. Daban and C.R. Cantor, Use of Fluorescent Probes to Study Nucleosomes.

M. Bustin, Preparation and Application of Immunological Probes for Nucleosomes.

S. Muller and M.H.V. Van Regenmortel, Use of Antihistone Antibodies with Nucleosomes.

L.C. Lutter, Digestion of Nucleosomes with Deoxyribonucleases I and II.

C. Wu, Analysis of Hypersensitive Sites in Chromatin.

M.E. Cusick, P.M. Wassarman, and M.L. DePamphilis, Application of Nucleases to Visualizing Chromatin Organization at Replication Forks.

M. Bellard, D. Dretzen, A. Giangrande, and P. Ramain, Nuclease Digestion of Transcriptionally Active Chromatin.

J.M. Gottesfeld, Analysis of RNA Polymerase III Transcription in Vitro Using Chromatin and Cloned Gene Template


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