Nuclear Particles

Nuclear Particles

The Cell Nucleus, Vol. 9

1st Edition - January 28, 1981

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  • Editor: Harris Busch
  • eBook ISBN: 9781483216812

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Description

The Cell Nucleus, Volume IX: Nuclear Particles, Part B discusses "splicing", "processing", and the controls of transcriptional and transport events which must be essential to cells that are either growing or are phenotypically differentiated. The book describes the characteristics and structure of nuclear 30 S RNP particles; as well as the composition and general topology of RNA and protein in monomer 40 S ribonucleoprotein particles. The text also demonstrates the two-dimensional gel electrophoresis of nuclear particles and immunocytochemistry of nuclear hnRNP complexes; the enzymatic activities associated with hnRNP; and the digestion products of nuclear ribonucleoprotein. The nucleocytoplasmic transport of mRNA; the nucleocytoplasmic transport of ribosomal subparticles; and the rnp particles involved in release of in vitro synthesized poly(a)-containing RNA in isolated nuclei are also considered. The book further tackles nuclear bodies as functional indicators in the target cells of sex steroid hormone; and the functional implications of nuclear glycoproteins and glycosaminoglycans. Cell biologists, geneticists, pharmacologists, molecular biologists, biochemists, and students taking related courses will find the book invaluable.

Table of Contents


  • List of Contributors

    Preface

    Contents of Other Volumes

    Chapter 1 Nuclear 30 S RNP Particles

    I. Isolation and Characterization of Nuclear RNP Particles

    II. Characteristics of the Protein and RNA Moieties of 30 S Particles

    III. Structure of Nuclear RNP Complexes

    IV. Conclusion: Structural Organization

    References

    Chapter 2 The Composition and General Topology of RNA and Protein in Monomer 40 S Ribonucleoprotein Particles

    I. Introduction

    II. Composition of Monomer 40 S hnRNP Particles

    III. The Arrangement of RNA and Protein in Monomer 40 S hnRNP Particles

    IV. Summary and a Possible Mechanism for 40 S hnRNP Function in RNA Splicing

    References

    Chapter 3 Two-Dimensional Gel Electrophoresis of Nuclear Particles

    I. Introduction

    II. Two-Dimensional Gel Electrophoresis Procedure

    III. Standardization of Gels

    IV. Saline-EDTA and Tris Washers of Nuclei

    V. Nuclear Matrix Proteins

    VI. Nuclear Membrane-Lamina Complex

    VII. Nucleolar Matrix

    VIII. Proteins of hnRNA

    IX. Proteins of Whole Nuclei and Chromatin

    X. Association of hnRNP with the Nuclear Matrix

    XI. Possible Gene Duplication of NHP

    XII. Silver Staining of Two-Dimensional Gels

    XIII. Summary

    References

    Chapter 4 Immunocytochemistry of Nuclear hnRNP Complexes

    I. hnRNP Complexes

    II. Localization of 30 S RNP Core Proteins

    III. Summary: hnRNP Core Proteins and Nuclear RNA Metabolism

    References

    Chapter 5 Enzymatic Activities Associated with hnRNP

    I. Introductory Remarks

    II. Enzymes Involved in Posttranscriptional Maturation of hnRNA into mRNA

    III. Enzymes Involved in Posttranslational Modifications of hnRNP Proteins

    IV. Concluding Remarks

    References

    Chapter 6 Digestion Products of Nuclear Ribonucleoprotein

    I. Introduction

    II. Initial Work Utilizing Nucleases

    III. Studies Involving Specific Nucleotide Sequences

    IV. Concluding Remarks

    References

    Chapter 7 Nucleocytoplasmic Transport of mRNA

    I. Introduction

    II. Processing as a Prerequisite for Transport

    III. Transport through the Nuclear Pore

    IV. Evidence for Regulation of mRNA Transport

    V. Evidence for Defective Regulation of mRNA Transport

    VI. Possible Mechanism of Regulation—mRNA-Associated Proteins

    References

    Chapter 8 Nucleocytoplasmic Transport of Ribosomal Subparticles: Interplay with the Nuclear Envelope

    I. Introduction

    II. Tetrahymena: Nuclear System and Extrachromosomal rRNA Genes

    III. Nucleocytoplasmic rRNP Transport

    IV. The Nuclear Envelope

    V. rRNP Translocation through Pore Complexes

    VI. Conclusion

    References

    Chapter 9 RNP Particles Involved in Release of in Vitro Synthesized Poly(A)-Containing RNA in Isolated Nuclei

    I. Introduction

    II. Results and Discussion

    References

    Chapter 10 Nuclear Bodies as Functional Indicators in the Target Cells of Sex Steroid Hormones

    I. Introduction

    II. Effects of Estrogens on Estrogen-Induced Responses and Estrogen Receptor Binding in Relation to the Formation of Nuclear Bodies

    III. Evidence Related to Endocrine Control of Nuclear Body Formation in the Uterus

    IV. Some Tentative Conclusions and Speculations Concerning the Structure and Function of Nuclear Bodies

    References

    Chapter 11 Nuclear Glycoproteins and Glycosaminoglycans

    I. Evidence for the Presence of Nuclear Glycoproteins and Glycosaminoglycans

    II. Association of Carbohydrate-Containing Molecules with Nucleoli and Nucleosomes

    III. Functional Implications of Nuclear Glycoproteins and Glycosaminoglycans

    References

    Index


Product details

  • No. of pages: 394
  • Language: English
  • Copyright: © Academic Press 1981
  • Published: January 28, 1981
  • Imprint: Academic Press
  • eBook ISBN: 9781483216812

About the Editor

Harris Busch

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