Nuclear Mechanics and Genome Regulation

Nuclear Mechanics and Genome Regulation

1st Edition - September 10, 2010
  • Editor: G.V. Shivashankar
  • eBook ISBN: 9780123810106
  • Hardcover ISBN: 9780123810090

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In recent years new discoveries have made this an exciting and important field of research. This exhaustive volume presents comprehensive chapters and detailed background information for researchers working with in the field of nuclear mechanics and genome regulation.

Key Features

  • Both classic and state-of-the-art methods readily adaptable and designed to last the test of time
  • Relevant to clinicians and scientists working in a wide range of fields


Researchers and students in cell, molecular and developmental biology

Table of Contents

  • Section-A: Cell Nucleus: Organization & MechanoBiology

    1. Fluorescence Fluctuation Microscopy to reveal 3D-Architecture and Function in the Cell Nucleus
    2. Thorsten Lenser1,2, Klaus Weisshart2, Tobias Ulbricht3, Karolin Klement3, and Peter Hemmerich

    3. Studying histone modifications and their genomic functions by employing chromatin immunoprecipitation and immunoblotting
    4. Ranveer S. Jayani, Praveena L. Ramanujam and Sanjeev Galande

    5. Dynamic organization of chromatin assembly & transcription factories in living cells
    6. Bidisha Sinha, Dipanjan Bhattacharya, Deepak Kumar Sinha, Shefali Talwar,

      Shovamayee Maharana, Soumya Gupta and G. V.Shivashankar


    7. Manipulation & Isolation of Single Cells & Nuclei
    8. Swee Jin Tan, Qingsen Li and Chwee Teck Lim

    9. Beyond lamins: other structural components of the nucleoskeleton
    10. Zhixia Zhong , Katherine L. Wilson and Kris Noel Dahl

    11. Altered mechanical properties of the nucleus in disease
    12. Maria Lucia Lombardi and Jan Lammerding

    13. Theoretical concepts and models of cellular mechanosensing
    14. Rumi De, Assaf Zemel and Samuel A. Safran

      Section-B: Impact of Nuclear Mechanics on Function

    15. Mechanical Induction of Gene Expression in Connective Tissue Cells
    16. Mathew Chan, Boris Hinz, Christopher A. McCulloch

    17. Physical plasticity of the Nucleus and its manipulation
    18. Irena Ivanovska, Joe Swift, Takamasa Harada, J. David Pajerowski and Dennis E. Discher

    19. Prestressed Nuclear Organization in Living Cells
    20. Aprotim Mazumder, Roopa T. , Abhishek Kumar, K.Venkatesan Iyer,

      Nisha M Ramdas and G. V.Shivashankar

    21. Nanotopography/mechanical induction of stem-cell differentiation
    22. Evelyn Yim


    23. Mechanical Induction in Embryonic Development and Tumour Growth: Integrative Cues through Molecular to Multi-cellular Interplay, and Evolutionary Perspectives
    24. Maria-Elena Fernandez-Sanchez, Fanny Serman, Padra Ahmadi and Emmanuel Farge

    25. Informatics based analysis of mechanosignaling in the laminopathies
    26. Frank P.L. Lai, Radfidah A. Mutalif, Siew Cheng Phua, and Colin L. Stewart

    27. Autosomal Dominant Leukodystrophy caused by lamin B1 duplications: A clinical and molecular case study of altered nuclear function and disease

    Quasar Saleem Padiath and Ying-Hui Fu

Product details

  • No. of pages: 392
  • Language: English
  • Copyright: © Academic Press 2010
  • Published: September 10, 2010
  • Imprint: Academic Press
  • eBook ISBN: 9780123810106
  • Hardcover ISBN: 9780123810090

About the Serial Volume Editor

G.V. Shivashankar

Dr. G. V. Shivashankar is currently the Deputy Director of Mechanobiology Institute, National University of Singapore. Shivashankar’s laboratory is focused on understanding the role of cell geometry on nuclear mechanics and genome regulation in living cells using a multi-disciplinary approach. He carried out his PhD research at the Rockefeller University (1994-1999) and Postdoctoral research at NEC Research Institute, Princeton, USA (1999-2000). He started his laboratory at the National Center for Biological Sciences, TIFR- Bangalore, India (2000-2009) before relocating to a tenured faculty position at the National University of Singapore in 2009. His scientific awards include the Birla Science Prize (2006) and the Swarnajayanthi Fellowship (2007), and he was elected to the Indian Academy of Sciences (2010). He also edited a Methods in Cell Biology volume on Nuclear Mechanics and Genome Regulation (2010), published by Elsevier. More recently, he headed the Joint Research Laboratory with the FIRC Institute of Molecular Oncology (IFOM), Milan, Italy and was appointed as an IFOM-NUS Chair Professor in 2014.

Affiliations and Expertise

Mechanobiology Institute, National University of Singapore & FIRC Institute of Molecular Oncology (IFOM), Milan, Italy