Nuclear magnetic Resonance in biochemistry - 1st Edition - ISBN: 9780123809506, 9780323141048

Nuclear magnetic Resonance in biochemistry

1st Edition

Authors: Thomas James
eBook ISBN: 9780323141048
Imprint: Academic Press
Published Date: 28th June 1975
Page Count: 428
Tax/VAT will be calculated at check-out Price includes VAT (GST)
30% off
30% off
30% off
30% off
30% off
20% off
20% off
30% off
30% off
30% off
30% off
30% off
20% off
20% off
30% off
30% off
30% off
30% off
30% off
20% off
20% off
54.95
38.47
38.47
38.47
38.47
38.47
43.96
43.96
72.95
51.06
51.06
51.06
51.06
51.06
58.36
58.36
43.99
30.79
30.79
30.79
30.79
30.79
35.19
35.19
Unavailable
Price includes VAT (GST)
× DRM-Free

Easy - Download and start reading immediately. There’s no activation process to access eBooks; all eBooks are fully searchable, and enabled for copying, pasting, and printing.

Flexible - Read on multiple operating systems and devices. Easily read eBooks on smart phones, computers, or any eBook readers, including Kindle.

Open - Buy once, receive and download all available eBook formats, including PDF, EPUB, and Mobi (for Kindle).

Institutional Access

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.

Description

Nuclear Magnetic Resonance in Biochemistry: Principles and Applications focuses on the principles and applications of nuclear magnetic resonance (NMR) in biochemistry. Topics covered include experimental methods in NMR; the mechanisms of NMR relaxation; chemical and paramagnetic shifts; spin-spin splitting; the use of NMR in investigations of biopolymers and biomolecular interactions; and molecular dynamics in biological and biochemical systems. This text is comprised of eight chapters; the first of which gives an overview of NMR spectroscopy and its use in studies of biological systems. The next two chapters discuss the theoretical basis for NMR applications in biochemistry, with emphasis on Bloch equations, quantum mechanics, correlation function and correlation time, double resonance, and chemical exchange. The reader is then introduced to the basis for chemical shifts and spin-spin splitting, along with several examples of the use of these NMR parameters in studies of small molecule interactions and structure. The experimental apparatus and procedures employed in NMR studies, Fourier transform NMR, and NMR spectral parameters of small molecules interacting with macromolecules are also considered. The book highlights the information obtainable from the spectra of biopolymers, and then concludes with a chapter on NMR investigations of the state of motion of lipids in membranes and model membranes; water in macromolecular and cellular systems; and sodium ion in biological tissue. This book is intended primarily for chemists, biochemists, biophysicists, and molecular biologists, as well as graduate students.

Table of Contents


Preface

Chapter 1 Introduction

1.1. Magnetic Properties of Nuclei

1.2. Magnetic Resonance

1.3. NMR Relaxation

1.4. Chemical Shift and Shielding

1.5. Spin-Spin Splitting

1.6. General Features of the NMR Spectrum

References

Chapter 2 Principles of Nuclear Magnetic Resonance

2.1. Classical Description—Bloch Equations

2.2. Quantum Mechanical Description of NMR

2.3. Correlation Function and Correlation Time

2.4. Relaxation Mechanisms

2.5. Double Resonance

2.6. Chemical Exchange

References

Chapter 3 Chemical Shifts and Structure

3.1. Shielding

3.2. Chemical Shifts

3.3. Paramagnetic Shifts

References

Chapter 4 Spin-Spin Splitting and Structure

4.1. Origin of Spin-Spin Splitting

4.2. Analysis of NMR Spectra

4.3. Molecular Structure

References

Chapter 5 Experimental Methods

5.1. Detection of Nuclear Magnetic Resonance

5.2. High-Resolution Continuous Wave Spectrometer

5.3. Pulsed NMR Spectrometer

5.4. Fourier Transform NMR Spectrometer

5.5. Multiple Irradiation

5.6. Correlation Spectroscopy

5.7. Relaxation Time Measurements

5.8. Self-Diffusion Coefficient Measurements

5.9. Multiple-Pulse NMR Experiments

5.10. Sample Considerations

References

Chapter 6 NMR Studies of Biomolecular Interactions

6.1. Chemical Exchange

6.2. Paramagnetic Probes

6.3. Quadrupolar Nuclei

6.4. Binding of Small Diamagnetic Molecules to Macromolecules

6.5. Application of the Intermolecular Nuclear Overhauser Effect

6.6. Application of Chemically Induced Dynamic Nuclear Polarization

References

Chapter 7 NMR Spectra of Biopolymers

7.1. Proteins and Polypeptides

7.2. Nucleic Acids and Polynucleotides

7.3. Polysaccharides

References

Chapter 8 Molecular Dynamics in Biological and Biochemical Systems

8.1. Membranes and Membrane Models

8.2. Water in Biochemical and Biological Systems

8.3. State of Sodium and Potassium Ions in Biological Tissue

References

Appendix 1 List of Symbols

Appendix 2 Table of Nuclear Properties

Subject Index


Details

No. of pages:
428
Language:
English
Copyright:
© Academic Press 1975
Published:
Imprint:
Academic Press
eBook ISBN:
9780323141048

About the Author

Thomas James