Nonisotopic Probing, Blotting, and Sequencing - 2nd Edition - ISBN: 9780124262911, 9780080537665

Nonisotopic Probing, Blotting, and Sequencing

2nd Edition

Editors: Larry Kricka
Hardcover ISBN: 9780124262911
eBook ISBN: 9780080537665
Imprint: Academic Press
Published Date: 22nd May 1995
Page Count: 518
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Description

Labels, Labelling, Analytical Strategies and Applications. Nonradioactive Labelling Methods for Nucleic Acids. Detection of Alkaline Phosphatase Labels By Time-Resolved Fluorescence. Detection of Alkaline Phosphatase By Bioluminescense. Detection of DNA on Membranes with Alkaline Phosphatase Labelled Probes and Chemiluminescence Cspdr Substrate. Detection of Alkaline Phosphatase By Colorimetry. Detection of Alkaline Phosphatase By Chemiluminescence Using NADP, Ascorbate and Indoxyl Phosphates. Detection of Horseradish Peroxidase By Enhanced Chemiluminescence. Detection of Horseradish Peroxidase By Colorimetry. Detection of Glucose 6-Phosphate Dehydrogenase By Bioluminescence. Detection of Xanthine Oxidase By Enhhanced Chemiluminescence. Electrochemiluminescence Detection of PCR-Derived Nucleic Acids. Detection of Phosphors By Phosphorescence. Detection of Europium Cryptates By Fluorescence. Detection of Lanthanid Chelates By Time-Resolved Fluorescence. Detection of Lanthanide Chelates and Multiple Labelling Strategies Based on Time-Resolved Fluorescence. Detection of Acridinium Esters By Chemiluminescence. Detection of Energy Transfer and Fluorescence Quenching. Sequencing By Nonisotopic Methods. Chemiluminesence DNA Sequencing with 1, 2-Dioxetanes.

Key Features

@introbul:Key Features @bul:* Provides strategies and detailed procedures for labeling, blotting, and probing specific nucleic acid sequences and, with this edition, protein molecules

  • Gives protocols for nonisotopic DNA sequencing - new in this edition
  • Gives extensive, practical information
  • Presents background information for each method
  • Provides expert accounts from the inventor or developer of each method
  • Contains seven entirely new chapters
  • Covers all major types of nonisotopic procedures for labeling and detection

Readership

AUDIENCE: Researchers of molecular biology, cell biology, genetics, developmental biology, microbiology, clinical medicine, diagnostics, biochemistry, immunology, neurosciences, oncology, pharmacology in academic and industrial laboratories.

Table of Contents

Labels, Labelling, Analytical Strategies and Applications. Nonradioactive Labelling Methods for Nucleic Acids. Detection of Alkaline Phosphatase Labels By Time-Resolved Fluorescence. Detection of Alkaline Phosphatase By Bioluminescense. Detection of DNA on Membranes with Alkaline Phosphatase Labelled Probes and Chemiluminescence Cspdr Substrate. Detection of Alkaline Phosphatase By Colorimetry. Detection of Alkaline Phosphatase By Chemiluminescence Using NADP, Ascorbate and Indoxyl Phosphates. Detection of Horseradish Peroxidase By Enhanced Chemiluminescence. Detection of Horseradish Peroxidase By Colorimetry. Detection of Glucose 6-Phosphate Dehydrogenase By Bioluminescence. Detection of Xanthine Oxidase By Enhhanced Chemiluminescence. Electrochemiluminescence Detection of PCR-Derived Nucleic Acids. Detection of Phosphors By Phosphorescence. Detection of Europium Cryptates By Fluorescence. Detection of Lanthanid Chelates By Time-Resolved Fluorescence. Detection of Lanthanide Chelates and Multiple Labelling Strategies Based on Time-Resolved Fluorescence. Detection of Acridinium Esters By Chemiluminescence. Detection of Energy Transfer and Fluorescence Quenching. Sequencing By Nonisotopic Methods. Chemiluminesence DNA Sequencing with 1, 2-Dioxetanes.

Details

No. of pages:
518
Language:
English
Copyright:
© Academic Press 1995
Published:
Imprint:
Academic Press
eBook ISBN:
9780080537665
Hardcover ISBN:
9780124262911

About the Editor

Larry Kricka

Affiliations and Expertise

Hospital of the University of Pennsylvania, Philadelphia, U.S.A.