SECTION I. BIOCHEMICAL, MOLECULAR, AND REAL-TIME DETECTION OF NITRIC OXIDE 1. The preparation of anaerobic nitric oxide solutions for the study of heme model systems in aqueous and non-aqueous media. Some consequences of NOx impurities 2, The NOtizer – A device for the convenient preparation of diazen-1-ium-1,2-diolates 3, Quantum Mechanical Determinations of Reaction Mechanisms, Acid Base and Redox Properties of Nitrogen Oxides and their Donors 4, Electrochemical and spectrophotometric methods for evaluation of NO dissociation rate constants from nitrosyl metal complexes activated through reduction 5. Sensitive and isotope-selective (14NO/15NO) on-line detection of nitric oxide by Faraday - Laser Magnetic Resonance Spectroscopy (LMRS) 6. Qualitative and quantitative determination of nitrite and nitrate with ion chromatography 7. Electrochemical Detection of Nitric Oxide in Biological Fluids 8. Simultaneous Detection of NO and ROS by ESR in Biological Systems 9. The ESR method to determine nitric oxide in plants
SECTION II. NITRATION AND S-NITROSYLATION 10, S-Nitrosothiol Formation 11. Detection and characterization of protein nitrosothiols 12. Detection of nitrosothiols and other nitroso species in vitro and in cells 13. Detection and identification of S-nitrosylated proteins in endothelial cells 14. S-Nitrosylation in Parkinson’s disease and related neurodegenerative disorders 15. Dynamic assessment of nitration reactions in vivo 16. Protein nitration in biological aging: proteomic and tandem mass spectrometric characterization of nitrated sites 17. HPLC-Electrochemical detection of tocopherol products as indicators of reactive nitrogen intermediates 18. Hydrophobic tyrosyl probes for monitoring nitration reactions in membranes
SECTION III: PEROXYNITRITE 19. Synthesis of peroxynitrite from nitrite and hydrogen peroxide 20. Peroxynitrite-dependent upregulation of Src kinases in red blood cells. Strategies to study the activation mechanisms 21. Hemoglobin and red blood cells as tools for studying peroxynitrite biochemistry 22. Quantification of 3-nitrotyrosine levels using a bench top ion trap mass spectrometry method 23. Mapping sites of tyrosine nitration by matrix-assisted laser desorption/ionization mass spectrometry 24. Peroxynitrite in the pathogenesis of Parkinson’s disease and the neuroprotective Role of metallothioneins
SECTION IV: SIGNALING AND GENE EXPRESSION 25. Yeast model system for examining nitrogen oxide biochemistry/signaling 26. Fluorescence resonance energy transfer based assays for real time detection of nitric oxide signaling 27. Nitric oxide is a signaling molecule that regulates gene expression 28. NO signaling in ARE-mediated gene expression 29. Tyrosine phosphorylation in nitric oxide-mediated signaling events 30. Identification and evaluation of NO regulated genes by differential analysis of primary cDNA library expression (DAzLE) 31. Role of NO in enhancing the expression of HO-1 in LPS-stimulated macrophages 32. G protein signaling in iNOS gene expression 33. Determination of nitric oxide donor effects on tissue gene expression in vivo using low density gene arrays
SECTION V: CELL BIOLOGY AND PHYSIOLOGY 34. Cell H2O2 steady-state concentration and mitochondrial nitric oxide 35. Cytotoxic and cytoprotective actions of O2- and NO (ONOO-) are determined both by cellular GSH level and HO activity in macrophages 36. Determination of mitochondrial nitric oxide synthase activity 37. Functional activity of mitochondrial nitric oxide synthase 38. Tetrahydrobiopterin as combined electron and proton donor in nitric oxide biosynthesis: Cryogenic UV/Vis and EPR detection of reaction intermediates 39. Antisense-mediated knock-down of iNOS expression in the presence of cytokines 40. Soluble Guanylyl Cyclase: The nitric oxide receptor 41. Purification and characterization of NO-sensitive guanylyl cyclase 42. The Measurement of Nitric Oxide Production by Cultured Endothelial Cells 43. Use of microdialysis to study interstitial nitric oxide and other reactive oxygen and nitrogen species in skeletal muscle 44. Nitric oxide, proteasomal function, and iron homeostasis: Implications in aging and neurodegenerative diseases 45. Nitric oxide production in primary liver cells isolated from amino acid diet fed rats 46. Update on nitric oxide-dependent vasodilation in human subjects 47. Assessing NO-dependent vasodilatation using vessel bioassays at defined oxygen tensions 48. Non-enzymatic nitric oxide formation during UVA-irradiation of human skin: Experimental setups and ways to measure 49. In vivo evaluation of NO-dependent apoptosis by NMR techniques 50. Microelectrode for in vivo real-time detection of NO 51. Real-time detection of nitric oxide isotopes in lung function tests 52. ESR techniques for the detection of nitric oxide in in vivo and in tissues
Since the inception of the series, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all field of life sciences.
This final volume in the five-part Nitric Oxide series supplements MIE volumes 268, 269, 301 and 359. Nitric Oxide impinges on a wide range of fields in biological research, particularly in the areas of biomedicine and cell and organic biology, as well as fundamental chemistry. These volumes are a valuable resource for the experienced researcher and for those entering the field.
*One of the most highly respected publication in the field of biochemistry since 1955 *Frequently consulted and praised by researchers and reviewers alike *Truly an essential publication for anyone in any field of the life sciences
Biochemists, biophysicists, cell biologists, molecular biologists, geneticists, developmental biologists
- No. of pages:
- © Academic Press 2005
- 18th August 2005
- Academic Press
- Hardcover ISBN:
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Praise for the Series: "The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE "It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY
University of Southern California, Los Angeles, USA
Lester Packer received a PhD in Microbiology and Biochemistry in 1956 from Yale University. In 1961, he joined the University of California at Berkeley serving as Professor of Cell and Molecular Biology until 2000, and then was appointed Adjunct Professor, Pharmacology and Pharmaceutical Sciences, School of Pharmacy at the University of Southern California.
Dr Packer received numerous distinctions including three honorary doctoral degrees, several distinguished Professor appointments. He was awarded Chevalier de l’Ordre National du Merite (Knight of the French National Order of Merit) and later promoted to the rank of Officier. He served as President of the Society for Free Radical Research International (SFRRI), founder and Honorary President of the Oxygen Club of California.
He has edited numerous books and published research; some of the most cited articles have become classics in the field of free radical biology:
Dr Packer is a member of many professional societies and editorial boards. His research elucidated - the Antioxidant Network concept. Exogenous lipoic acid was discovered to be one of the most potent natural antioxidants and placed as the ultimate reductant or in the pecking order of the “Antioxidant Network” regenerating vitamins C and E and stimulating glutathione synthesis, thereby improving the overall cellular antioxidant defense. The Antioxidant Network is a concept addressing the cell’s redox status. He established a world-wide network of research programs by supporting and co-organizing conferences on free radical research and redox biology in Asia, Europe, and America.
Department of Molecular Pharmacology and Toxicology, School of Pharmaceutical Sciences, University of Southern California, USA
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