SECTION I. BIOCHEMICAL, MOLECULAR, AND REAL-TIME DETECTION OF NITRIC OXIDE1. The preparation of anaerobic nitric oxide solutions for the study of heme model systems in aqueous and non-aqueous media. Some consequences of NOx impurities 2, The NOtizer – A device for the convenient preparation of diazen-1-ium-1,2-diolates 3, Quantum Mechanical Determinations of Reaction Mechanisms, Acid Base and Redox Properties of Nitrogen Oxides and their Donors 4, Electrochemical and spectrophotometric methods for evaluation of NO dissociation rate constants from nitrosyl metal complexes activated through reduction 5. Sensitive and isotope-selective (14NO/15NO) on-line detection of nitric oxide by Faraday - Laser Magnetic Resonance Spectroscopy (LMRS) 6. Qualitative and quantitative determination of nitrite and nitrate with ion chromatography 7. Electrochemical Detection of Nitric Oxide in Biological Fluids 8. Simultaneous Detection of NO and ROS by ESR in Biological Systems 9. The ESR method to determine nitric oxide in plants
SECTION II. NITRATION AND S-NITROSYLATION 10, S-Nitrosothiol Formation11. Detection and characterization of protein nitrosothiols 12. Detection of nitrosothiols and other nitroso species in vitro and in cells 13. Detection and identification of S-nitrosylated proteins in endothelial cells 14. S-Nitrosylation in Parkinson’s disease and related neurodegenerative disorders 15. Dynamic assessment of nitration reactions in vivo 16. Protein nitration in biological aging: proteomic and tandem mass spectrometric characterization of nitrated sites 17. HPLC-Electrochemical detection of tocopherol products as indicators of reactive nitrogen intermediates 18. Hydrophobic tyrosyl probes for moni
Since the inception of the series, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all field of life sciences.
This final volume in the five-part Nitric Oxide series supplements MIE volumes 268, 269, 301 and 359. Nitric Oxide impinges on a wide range of fields in biological research, particularly in the areas of biomedicine and cell and organic biology, as well as fundamental chemistry. These volumes are a valuable resource for the experienced researcher and for those entering the field.
One of the most highly respected publication in the field of biochemistry since 1955 Frequently consulted and praised by researchers and reviewers alike *Truly an essential publication for anyone in any field of the life sciences
Biochemists, biophysicists, cell biologists, molecular biologists, geneticists, developmental biologists
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- 18th August 2005
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Praise for the Series: "The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE "It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY
University of Southern California, Los Angeles, USA
Lester Packer received a PhD in Microbiology and Biochemistry in 1956 from Yale University. In 1961, he joined the University of California at Berkeley serving as Professor of Cell and Molecular Biology until 2000, and then was appointed Adjunct Professor, Pharmacology and Pharmaceutical Sciences, School of Pharmacy at the University of Southern California. Dr Packer received numerous distinctions including three honorary doctoral degrees, several distinguished Professor appointments. He was awarded Chevalier de l’Ordre National du Merite (Knight of the French National Order of Merit) and later promoted to the rank of Officier. He served as President of the Society for Free Radical Research International (SFRRI), founder and Honorary President of the Oxygen Club of California. He has edited numerous books and published research; some of the most cited articles have become classics in the field of free radical biology: Dr Packer is a member of many professional societies and editorial boards. His research elucidated - the Antioxidant Network concept. Exogenous lipoic acid was discovered to be one of the most potent natural antioxidants and placed as the ultimate reductant or in the pecking order of the “Antioxidant Network” regenerating vitamins C and E and stimulating glutathione synthesis, thereby improving the overall cellular antioxidant defense. The Antioxidant Network is a concept addressing the cell’s redox status. He established a world-wide network of research programs by supporting and co-organizing conferences on free radical research and redox biology in Asia, Europe, and America.
Department of Molecular Pharmacology and Toxicology, School of Pharmaceutical Sciences, University of Southern California, USA