Description

Next Generation Sequencing demystifies the process of sequencing with a hands-on approach. Offering a theoretical and practical discussion of the how and why behind the kit culture, this publication instructs readers on the science behind the technology and the analysis and application of data. The primer includes a needed pros-and-cons list, protocols, and a discussion of implementing applications.

Filling the gap in next generation sequencing literature, Next Generation Sequencing: A Primer provides needed instruction for early researchers, students and researchers outside of their expertise across the biological and biomedical sciences.

Key Features

  • Provides information on how technology works
  • Discusses how and why to prepare samples
  • Instructs on how to analyze data
  • Covers basic techniques and history behind the kit culture

Readership

Students, graduate students, postdocs and researchers in molecular biology, biochemistry, and genetics and across the life and biomedical sciences

Table of Contents

Introduction

Current next generation technologies, clonal approaches, single molecule approaches

Rationale: NGS enables new biological questions

Growth of cells for NGS (using yeast and cultured mammalian cells)

Nucleic acid extraction: DNA and RNA

PCR, gel electrophoresis and quantitative PCR

Overview of diverse types of NGS sequencing libraries.

Preparation of genomic DNA and RNA-seq libraries

Sequencer Operation: overview of diverse platforms

Sequencing on the MiSeq

Data extraction and analysis I: quality filters, trimming, demultiplexing and mapping

Data analysis II: genome viariants- SNPs, CNVs and rearrangements

Data analysis III: RNA seq analysis- gene expression, non-coding sequences, splicing

Data analysis IV: Data visualization and data sharing

Summary and report preparation

Applications of NGS

Perspectives: NEXT next generation technologies

Details

No. of pages:
240
Language:
English
Copyright:
© 2014
Published:
Imprint:
Academic Press
Print ISBN:
9780124077706
Electronic ISBN:
9780124078963