New Trends in Genetic Risk Assessment is based on the Fifth International Round Table of the Rhône-Poulenc Santé Foundation, held in Nice 1987. The conference was an attempt to review the latest theories and mechanisms stipulated for the various aspects of genotoxicity; it was above all an open forum to discuss the new trends, the new tests, and the new battery of tests for assessing the genetic risk of chemicals and especially drugs. This volume is actually not a proceedings of the meeting but a monograph specially edited to report the reviews which were presented and the discussions which took place; it was designed to provide a better understanding of the knowledge obtained in the most recent years and to help in the practical choice of approaches or tests for the prediction of the various forms of genotoxicity. The main subjects of this review concern molecular analysis of mutagenesis, detection of DNA damage, gene mutation, clastogenesis, aneuploidy, and germ cells. Each theme is preceded by a short overview summarizing the state of the art and the contributions of each author. Finally, special attention was given to the personal views of some leading toxicologists as to the battery of tests presently available or recommended.
Contributors Participants Preface Acknowledgments Introduction 1 The Role of Genotoxicity in Drug Safety Evaluation Part I - Molecular Analysis of Mutagenesis Introduction 2 DNA Repair and Genetic Alterations 3 Molecular Approaches to the Detection of Germinal Mutations in Mammalian Organisms, Including Man 4 Molecular analysis of Genetic and Epigenetic Alterations in Human Cells 5 Artificial Intelligence in the Study of the Structural Relationships Amongst Mutagens and Carcinogens Part II - Detection of DNA Damage Genotoxicity and Covalent Binding. An Introduction 6 Fluorimetric Analysis of DNA-strand Breakage and Repair Kinetics. Application to Radiotoxicology 7 Detection by 32P-postlabeling of Carcinogen-DNA Adducts in Animals and Man 8 SOS Chromotest and the Use of Bacteria for the Detection and Diagnosis of Genotoxic Agents Part III - Gene Mutation Introduction 9 Mutation at the hprt Locus in Human T-lymphocytes: In Vivo and In Vitro Studies 10 Mutagenicity and Clastogenicity Testing with Mouse Lymphoma Cells 11 Measurement of Mutant Frequency to 6-Thioguanine Resistance in Circulating T-lymphocytes for Human Population Monitoring Part IV - Clastogenesis and Aneuploidy Introduction 12 Specific Sites of Chromosomal Radiationinduced Rearrangements 13 Flow Cytometric Analysis and Sorting of Chromosomes 14 Flow Cytometric Detection of Micronuclei 15 Induced Chromosome Aneuploidy: its Role in the Assessment of the Genetic Toxicology of Environmental Chemicals 16 Assessment of Chemicallyinduced Aneuploidy: an Assay System Using a Mouse/Human Hybrid Cell Line Part V - Germ Cells Introduction 17 Prospective Overview on Germ Cell Mutations in Mice 18 Flow Cytometric Analysis of Toxic Chemicalinduced Alterations in Testicular Cell Kinetics and Sperm Chromatin Structure Part VI - Test Selection and Risk Assessment Introd
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- © Academic Press 1989
- 28th January 1989
- Academic Press
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Rhone-Poulenc Rorer, Antony, France