New Approaches for Flavin Catalysis

New Approaches for Flavin Catalysis

1st Edition - May 7, 2019

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  • Editor: Bruce Palfey
  • eBook ISBN: 9780128168301
  • Hardcover ISBN: 9780128168295

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New Approaches for Flavin Catalysis, Volume 620, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered in this update include Anaerobiosis and Methods for Reduction, Reduction Potentials, Anaerobic Stopped-Flow, No Glove-Box, Anaerobic Stopped-Flow, in a Glove-Box, Chemical Quenching, Oxygen Reactions, Double-mixing Stopped-Flow, Kinetic Isotope Effects and Viscosity Effects, Heavy Enzymes Synthetic Flavins & Linear Free Energy Relationships, Vibrational Spectroscopy, Stark Spectroscopy, EPR and Related Methods, Molecular Dynamics, Phylogenetic Relationships/Superfamilies, O2 and Superoxide Analogs, and more.

Key Features

  • Provides the authority and expertise of leading contributors from an international board of authors
  • Presents the latest release in the Methods in Enzymology series
  • Updated release includes the latest information on New Approaches for Flavin Catalysis


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • 1. Methods for determining the reduction potentials of flavin enzymes
    Shelbi L. Christgen, Sophia M. Becker and Donald F. Becker
    2. Anaerobic methods for the transient-state study of flavoproteins: The use of specialized glassware to define the concentration of dioxygen
    Graham R. Moran
    3. Performing anaerobic stopped-flow spectrophotometry inside of an anaerobic chamber
    Hannah Valentino and Pablo Sobrado
    4. Chemical quenching and identification of intermediates in flavoenzyme-catalyzed reactions
    Kalani Karunaratne and Tatiana V. Mishanina
    5. On the use of noncompetitive kinetic isotope effects to investigate flavoenzyme mechanism
    Kevin Francis and Giovanni Gadda
    6. Isotopically labeled flavoenzymes and their uses in probing reaction mechanisms
    Andreea I. Iorgu, Matthew J. Cliff, Jonathan P. Waltho, Nigel S. Scrutton and Sam Hay
    7. Unraveling flavoenzyme reaction mechanisms using flavin analogues and linear free energy relationships
    Christopher J. Thibodeaux, Wei-chen Chang and Hung-wen Liu
    8. Vibrational spectroscopy of flavoproteins
    James N. Iuliano, Jarrod B. French and Peter J. Tonge
    9. Measuring electronic structure properties of flavins and flavoproteins by electronic stark spectroscopy
    Robert J. Stanley and Cornelius van Galen
    10. EPR spectroscopy on flavin radicals in flavoproteins
    Daniel Nohr, Stefan Weber and Erik Schleicher
    11. Applications of molecular modeling to flavoproteins: Insights and challenges
    Emil Sjulstok, Ilia A. Solov'yov and Peter L. Freddolino
    12. Exploring the sequence, function, and evolutionary space of protein superfamilies using sequence similarity networks and phylogenetic reconstructions
    Janine N. Copp, Dave W. Anderson, Eyal Akiva, Patricia C. Babbitt and Nobuhiko Tokuriki
    13. Structural methods for probing the interaction of flavoenzymes with oxygen and its surrogates
    Raspudin Saleem-Batcha and Robin Teufel
    14. Reduction midpoint potentials of bifurcating electron transfer flavoproteins
    Anne-Frances Miller, H. Diessel Duan, Taylor A. Varner and Nishya M. Raseek
    15. Investigations of two-component flavin-dependent monooxygenase systems
    John M. Robbins and Holly R. Ellis
    16. The styrene monooxygenase system
    George T. Gassner
    17. Flavin-N5-oxide intermediates in dibenzothiophene, uracil, and hexachlorobenzene catabolism
    Sanjoy Adak and Tadhg P. Begley
    18. Prenylated FMN: Biosynthesis, purification, and Fdc1 activation
    Anna N. Khusnutdinova, Johnny Xiao, Po-Hsiang Wang, Khorcheska A. Batyrova, Robert Flick, Elizabeth A. Edwards and Alexander F. Yakunin
    19. Heterologous production, reconstitution and EPR spectroscopic analysis of prFMN dependent enzymes
    Stephen Marshall, Karl A.P. Payne, Karl Fisher, Deepankar Gahloth, Samuel S. Bailey, Arune Balaikaite, Annica Saaret, Irina Gostimskaya, Godwin Aleku, Huanming Huang, Stephen E.J. Rigby, David Procter and David Leys
    20. Physical methods for studying flavoprotein photoreceptors
    Estella F. Yee, Siddarth Chandrasekaran, Changfan Lin and Brian R. Crane

Product details

  • No. of pages: 564
  • Language: English
  • Copyright: © Academic Press 2019
  • Published: May 7, 2019
  • Imprint: Academic Press
  • eBook ISBN: 9780128168301
  • Hardcover ISBN: 9780128168295

About the Serial Volume Editor

Bruce Palfey

Dr. Bruce A. Palfey teaches at the Department of Biological Chemistry of the University of Michigan Medical School, U.S.A.

Affiliations and Expertise

University of Michigan Medical School, USA

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