General Description of the Series:
Neurotransmitter Transporters focuses on biochemical, electrophysiological, pharmacological, molecular, and cell biological approaches used to study neurotransmitter transport systems. The articles provide detailed descriptions of procedures that should enable the reader to understand how they are accomplished and to repeat or adapt them for their own experimental needs. This book is the first to focus on methods that have been the basis for the rapid development of this area.
General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
@introbul:Key Features @bul:* The transport of CNS neurotransmitter transporters
- Electrophysiological, biochemical, molecular, cellular biological, pharmacological, neurochemical, and structural approaches
- Both plasma and vesicular carriers
Biochemists, neuroscientists, physiologists, molecular and cell biologists, pharmacologists, and geneticists.
Purification of Transporter Proteins and cDNAs:
Plasma Membrane Carriers:
C. Aragon and B. Lopez-Corcuera, Purification, Hydrodynamic Properties, and Glycosylation Analysis of Glycine Transporters.
M.F. Romero, Y. Kanai, H. Gunshin, and M.A. Hediger, Expression Cloning Using Xenopus laevis Oocytes.
N. Nelson and Q.R. Liu, Cloning of Genes of cDNAs Encoding Neurotransmitter Transporters and their Localization by Immunocytochemistry.
R. Yelin and S. Schuldiner, Purification of the Vesicular Monoamine Transporters: From Classical Techniques to Histidine Tags.
J.P. Henry, C. Sagné, M.F. Isambert, and B. Gasnier, Noncovalent and Covalent Labeling of the Vesicular Monoamine Transporter with Tetrabenazine and Ketanserin Derivatives; Purification of the Photolabeled Protein.
H. Varoqui and J.D. Erickson, Functional Identification of Vesicular Monoamine and Acetylcholine Transporters.
S.M. Parsons, G.A. Rogers, and L.M. Gracz, Photoaffinity Labeling of the Vesicular Acetylcholine Transporter from the Electric Organ of Torpedo.
J.W. Hell and R. Jahn, Bioenergetic Characterization of the GABA Transporter of Synaptic Vesicles.
S.M. Lewis and T. Ueda, Solubilization and Reconstitution of the Synaptic Vesicle Glutamate Transport System.
J.P. Finn, III, A. Merickel, and R.H. Edwards, Analysis of Neurotransmitter Transport into Secretory Vesicles. Pharmacological Approaches and Binding Studies:
P. Krogsgaard-Larsen, B.F. Frølund, and E. Falch, Inhibitors of GABA Transport as Experimental Tools and Therapeutic Agents.
A.R. Chamberlin, H.P. Koch, and R.J. Bridges, Design and Synthesis of the Con
- No. of pages:
- © Academic Press 1998
- 15th September 1998
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY