Neurophotonics and Biomedical Spectroscopy

Neurophotonics and Biomedical Spectroscopy

1st Edition - November 22, 2018

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  • Editors: Robert Alfano, Lingyan Shi
  • eBook ISBN: 9780323480680
  • Hardcover ISBN: 9780323480673

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Neurophotonics and Biomedical Spectroscopy addresses the novel state-of-the-art work in non-invasive optical spectroscopic methods that detect the onset and progression of diseases and other conditions, including pre-malignancy, cancer, Alzheimer’s disease, tissue and cell response to therapeutic intervention, unintended injury and laser energy deposition. The book then highlights research in neurophotonics that investigates single and multi-photon excitation optical signatures of normal/diseased nerve tissues and in the brain, providing a better understanding of the underlying biochemical and structural changes of tissues and cells that are responsible for the observed spectroscopic signatures. Topics cover a wide array of well-established UV, visible, NIR and IR optical and spectroscopic techniques and novel approaches to diagnose tissue changes, including: label free in vivo and ex vivo fluorescence spectroscopy, Stoke shift spectroscopy, spectral imaging, Resonance Raman spectroscopy, multiphoton two Photon excitation, and more.

Key Features

  • Provides an overview of the spectroscopic properties of tissue and tissue-light interaction, describing techniques to exploit these properties in imaging
  • Explores the potential and significance of molecule-specific imaging and its capacity to reveal vital new information on nanoscale structures
  • Offers a concise overview of different spectroscopic methods and their potential benefits for solving diagnostic and therapeutic problems


Researchers in nanomedicine, nanophotonics and optics, biomedical optics, neuroscientists, molecular biologists, clinical neurologists, and optical physics

Table of Contents

  • Part 1: Topics in Optical Spectroscopy Biopsy
    1. Origin of tissue optical properties
    2. Optical methods for tissue diagnosis and treatment
    3. Methods for in vivo assessment of physiological state of tissue
    4. Excitation, absorption, scattering fluorescence spectroscopy
    5. Resonance Raman spectroscopy
    6. Inelastic light scattering spectroscopy and imaging
    7. Stimulated Raman gain spectroscopy and imaging—NIR optical windows in 700 nm to 2500 nm
    8. Coherent and non-contact photo-acoustic imaging
    9. Polarization and diffusive reflectance spectral imaging time resolved spectroscopy single and multi-photon excitation and imaging
    10. Mid infrared diagnostic methods
    11. Nano particle tagging and contrast agents optical metabolomics Demos, Gansean
    12. Supercontinuum for medical and biological applications
    13. Stokes shift spectroscopy

    Part 2: Topics in Optics in Brain
    14. Alzheimer’s Disease Optical Imaging in vascular impairment
    15. Hemodynamics by NIR in Brain, and Overview View summary of field
    16. Blood Barrier in mice using two photon and ultrasound
    17. Overview of Infrared neural stimulation
    18. 2Photon excitation in brain
    19. Overview NIR correlation spectroscopy in brain
    20. Overview of Optical Coherent Tomography in neuron tree
    21. IR stimulation in spinal nerves
    22. Ca+2 channels in brain by multiphotons
    23. Ca2+ flow in brain by multiphotons
    24. Overview Ca2+ indicators by 2PEF
    25. Control Biosystems using optogentics
    26. Optogenetic circuit mapping with traditional whole cell patch clamp to determine synaptic connectivity between identified neurons in mouse models of psychiatric disease
    27. Special Proteins Red fluorescent proteins RFP in brain probing (Dr. Robert Campbell)
    28. OCT neural activity
    29. Stimulated Raman Scattering Microscopy in Brain Research
    30. Imaging the neurovascular coupling system with laminar optical tomography and hyperspectral two-photon microscopy
    31. Multi photon Imaging in neuroscience

Product details

  • No. of pages: 610
  • Language: English
  • Copyright: © Elsevier 2018
  • Published: November 22, 2018
  • Imprint: Elsevier
  • eBook ISBN: 9780323480680
  • Hardcover ISBN: 9780323480673

About the Editors

Robert Alfano

Robert A. Alfano is Distinguished Professor and Fellow at The City College of City University of New York, USA. He is also an Optical Society of America; Fellow, and a Fellow of IEEE. He has been involved in developing ultrafast laser spectroscopic techniques and applications of these techniques to study ultrafast dynamical processes in physical, chemical, and biological systems. His research encompasses the study and development of supercontinuum, tunable solid-state lasers, nonlinear optical processes, application of optical spectroscopic techniques for medical diagnosis (optical biopsy), study of photon migration in turbid media, and development of optical imaging techniques for biomedical imaging (optical mammography). He has published more than 700 papers and holds 102 patents.

Affiliations and Expertise

Professor and Fellow, The City College of City University of New York, USA

Lingyan Shi

Lingyan Shi is an Assistant Professor in the Department of Bioengineering at the UC San Diego Jacobs School of Engineering. She is developing and applying new ultrafast laser scanning optical imaging and spectroscopic technologies, such as stimulated Raman scattering (SRS) microscopy for solving biological questions. Her recent major scientific contributions include the discovery of the “Golden Optical Window” for deep brain imaging, and the development of a breakthrough platform that combines deuterium probing with SRS for optical imaging of metabolic dynamics in living organisms. She is combining bioorthogonal labeling and SRS imaging for directly visualizing complex molecular events in brain, tumor, and metabolic diseases at sub-cellular scale in situ, which offer powerful tools potentially for disease detection, diagnosis and treatment, as well as for mechanistic understanding of scientific fundamentals. Shi is a member of OSA, SPIE, and also a member of New York Academy of Science.

Affiliations and Expertise

Research Scientist, Columbia University, USA

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