Neuronal and Glial Proteins

Neuronal and Glial Proteins

Structure, Function, and Clinical Application

1st Edition - January 28, 1987

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  • Editor: Paul Marangos
  • eBook ISBN: 9780323151566

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Neuronal and Glial Proteins: Structure, Function, and Clinical Application focuses on the basic and clinical information relating to a number of proteins that are either enriched in or unique to nervous tissue. This book discusses the structural and functional characteristics of cell-specific proteins, which provide a better understanding of the molecular mechanisms involved in processes that are specific to glia or neurons. Organized into three sections encompassing 15 chapters, this book starts with an overview of the fundamental principles and strategies involved in studying the anatomical, structural, functional, and immunological aspects of brain protein. This text then discusses the techniques, including the preparation of brain tissues as well as the preparation of neural and glial cells in purified form. Other chapters review the two-dimensional gel electrophoresis, which is recognized as a significant technique for discovering brain molecules. The final chapter deals with the membrane-associated nervous system proteins. Neurochemists and clinical researchers will find this book useful.

Table of Contents

  • Preface

    Section I Methods of Identifying and Modifying Brain Proteins

    1. Preparation of Cellular and Subcellular Fractions from the Central Nervous System

    I. Introduction

    II. Preparation of Subcellular Fractions

    III. Neural Cell Preparations

    IV. Bulk-Isolation of Cells from Brain

    V. Preparation of Plasma Membranes from Bulk-Isolated Cells

    VI. Summary


    2. The Use of Two-dimensional Gel Electrophoresis to Study Proteins in the Central Nervous System

    I. Introduction

    II. Methodology

    III. Studies on Central Nervous System Proteins Using 2DE

    IV. Clinical Application of 2DE in the Study of Central Nervous System Proteins

    V. Conclusions


    3. Immunological Approaches to the Identification of Cell Surface Specific Antigens in Brain

    I. Introduction

    II. Antibody Production

    III. Antibody Screening and Analysis

    IV. Antibodies in Neurobiology

    V. Monoclonal Antibodies Used to Study Tumors of the Nervous System

    VI. Clinical Applications of Monoclonal Antibodies against Nervous System Antigens


    4. Methods of Localizing Cell-Specific Proteins in Brain

    I. Introduction

    II. Cell-Specific Proteins—Measurement versus Localization

    III. Fixation, Processing, and Detection

    IV. Interpretation of Immunocytochemical Results

    V. Application—Developing Brain Tissue

    VI. Application—Tissue Culture

    VII. Application—Human Pathology

    VIII. Role of Localization with Other Methodologies


    5. Protein Phosphorylation: A Convergence Site for Multiple Effector Pathways

    I. Introduction

    II. Protein Kinases

    III. Protein Kinase Substrates

    IV. Multisite Phosphorylation of Enzymes

    V. Multisite Phosphorylation of Structural Proteins

    VI. Multisite Phosphorylation of Receptors

    VII. Closing Remarks

    VIII. Conclusions


    Section II Soluble Nervous System Proteins

    6. Neuron-Specific Enolase: A Neural and Neuroendocrine Protein

    I. Introduction

    II. Functional Characterization of the 14-3-2 Protein

    III. Structure of the Brain Enolases

    IV. Tissue Distribution and Cellular Localization of Neuron-Specific Enolase and Nonneuron Enolase

    V. Developmental and Molecular Biological Aspects of Neuron-Specific Enolase

    VI. Enzymologic Differences between Nonneuron Enolase and Neuron-Specific Enolase

    VII. Clinical Studies

    VIII. Concluding Remarks


    7. The S-100 Protein

    I. Introduction and History

    II. Purification of S-100

    III. Antibodies and Immunology

    IV. Distribution of S-100 in Neural and Nonneural Tissues

    V. Species Comparisons and Evolution

    VI. Development

    VII. Structure and Chemistry of S-100 Protein

    VIII. The S-100 Protein in Tumors

    IX. Function of S-100 Protein

    X. Summary and Future Work


    8. Growth Factors for the Nervous System

    I. Nerve Growth Factor

    II. Other Factors Acting on Cells from the Nervous System


    9. Protein Gene Product 9.5: A New Neuronal and Neuroendocrine Marker

    I. Introduction

    II. Properties of PGP 9.5

    III. Production of Antibodies to Human PGP 9.5

    IV. Possible Functional Roles and Primary Structure of PGP 9.5


    Section III Membrane-Associated Nervous System Proteins

    10. Structural, Functional, and Clinical Aspects of Myelin Proteins

    I. Introduction

    II. Myelin

    III. Major Protein Components of Central Nervous System Myelin

    IV. Proteins of Peripheral Nervous System Myelin

    V. Nonenzyme-Catalyzed Alterations of Myelin Proteins

    VI. Immunological Studies

    VII. Molecular Genetics

    VIII. Infectious Agents

    IX. Conclusions


    11. Myelin Proteolipid Protein

    I. Introduction

    II. Isolation and Identification

    III. Properties of Proteolipid Apoprotein

    IV. Biosynthesis of the Myelin Proteolipid Protein

    V. Posttranslational Modification: Acylation

    VI. Molecular and Genetic Approaches

    VII. Proteolipids in Disease

    VIII. Functions of the Myelin Proteolipid

    IX. Future Directions


    12. Myelin-Associated Glycoprotein: Functional and Clinical Aspects

    I. Introduction

    II. General Properties of Myelin-Associated Glycoprotein

    III. Functional Aspects

    IV. Clinical Aspects

    V. Conclusions


    13. Microtubules: Structural, Functional, and Clinical Aspects

    I. Introduction

    II. Methods Used in the Study of Microtubules

    III. Microtubule Assembly-Disassembly

    IV. Functions of Microtubules

    V. Clinical Aspects and Applications


    14. Glial Fibrillary Acidic Protein: A Review of Structure, Function, and Clinical Application

    I. Introduction

    II. Glial Fibrillary Acidic Protein Chemistry

    III. Glial Fibrillary Acidic Protein Immunochemistry

    IV. Glial Fibrillary Acidic Protein Metabolism

    V. Glial Fibrillary Acidic Protein in Reactive Astrogliosis

    VI. Vimentin in Central Nervous System Development and Reactive Astrogliosis

    VII. Glial Fibrillary Acidic Protein Clinical Studies

    VIII. Future Prospectives


    15. Thy-1: Possible Functions in Nervous and Immune Systems

    I. Introduction

    II. Evidence from Structure

    III. Evidence from Tissue Distribution

    IV. Evidence from Antibody Perturbation of Cellular Function

    V. Evidence from Genetic Manipulation

    VI. Conclusions and Prospects



Product details

  • No. of pages: 410
  • Language: English
  • Copyright: © Academic Press 1987
  • Published: January 28, 1987
  • Imprint: Academic Press
  • eBook ISBN: 9780323151566

About the Editor

Paul Marangos

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