Neuroanatomical Research Techniques - 1st Edition - ISBN: 9780125903509, 9781483266404

Neuroanatomical Research Techniques

1st Edition

Editors: Richard T. Robertson
eBook ISBN: 9781483266404
Imprint: Academic Press
Published Date: 1st January 1978
Page Count: 462
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Neuroanatomical Research Techniques discusses developments in major neuroanatomical research techniques. The book is organized into four parts. Part I deals generally with the preparation and study of brain tissue. It includes a chapter on the microscope, discussing optical magnification, limitations of microscopy, and optical contrasting methods. Other chapters summarize basic techniques for tissue preparation and sectioning; present guidelines for a number of standard, but essential, staining procedures; and present sophisticated and contemporary computer techniques that are proving to be invaluable as neuroanatomy evolves from a qualitative to a quantitative discipline. Part II deals with techniques often used for the study of normal tissue. These include the Golgi method, fluorescence histochemistry, techniques for staining single neurons, and the use of the electron microscope. Part III presents techniques for studying intrinsic connections of the nervous system. These include techniques for silver impregnation of degenerating fibers; autoradiographic technique for studying axonal projections; and somatopetal movement of horseradish peroxidase as a tool for studying connections and neuron morphology. Part IV discusses the interpretation of results from neuroanatomical research techniques and presents examples of the applications of neuroanatomical methods to major problems in physiological psychology.

Table of Contents

List of Contributors



Preparation and Study of Brain Tissues

Chapter 1 Microscopy and Photomicrography: an Introduction

I. The Use of the Microscope

II. Photographic Techniques

Chapter 2 Tissue Preparation and Basic Staining Techniques

I. Introduction

II. Tissue Fixation

III. Preparation of Tissues for Sectioning

IV. Mounting of Sections

V. Staining Procedures

VI. Conclusions


Chapter 3 Computer-Assisted Recording of Neuroanatomical Data

I. Introduction

II. Suitability of Computers to Recording Neuroanatomical Data

III. Methodology

IV. Appearance of the Data, Analysis, and Interpretation

V. Technical Problems, Methodological Limitations, and Interpretive Difficulties

VI. Summary and Comments


Techniques for the Study of Normal Tissue

Chapter 4 The Methods of Golgi

I. Introduction

II. Why Use the Golgi Methods?

III. Where is the Golgi Useful?

IV. How to Use the Golgi Methods

V. What are the Artifacts and Pitfalls?

VI. Rationale of the Golgi Methods

VII. Interpretation of the Data


Chapter 5 Fluorescence Histochemistry

I. Introduction

II. Fluorescence Histochemical Methods for Demonstrating Biogenic Amines in Neurons

III. Conclusions


Chapter 6 Single-Cell Staining Techniques

I. Introduction

II. Microelectrode Injection

III. Axonal Iontophoresis: Procedure

IV. Tissue Processing for Light Microscopy

V. Ultrastructural Use of Intracellular Dyes

VI. Possible Limitations of Intracellular Staining

VII. Prospectus

VIII. Addendum


Chapter 7 Electron Microscopy and the Study of the Ultrastructure of the Central Nervous System

I. Introduction

II. Specimen Preparation

III. Additional Selective Methods for Electron Microscopy

IV. Ultrastructure: The Interpretation of the Electron Microscopic Image


Study of Connections in the Nervous System

A. Techniques Based on Orthograde Processes

Chapter 8 The Study of Degenerating Nerve Fibers Using Silver-Impregnation Methods

Chapter 9 The Use of Axonal Transport for Autoradiographic Tracing of Pathways in the Central Nervous System

Chapter 10 The Use of Somatofugal Transport of Horseradish Peroxidase for Tract Tracing and Cell Labeling

Chapter 11 Neurochemical Effects of Lesions

B. Techniques Based on Retrograde Processes

Chapter 12 Analysis of Retrograde Degeneration in Cell Soma following Axon Transection

Chapter 13 A Review of the Retrograde Transport Technique

C. Electrophysiological Techniques

Chapter 14 Electrophysiological Mapping Techniques


Chapter 15 Histological Techniques in Neuropsychology: The Past and Some Trends for the Future

I. Introduction

II. Some Examples of Experiments with Simultaneous Anatomical and Behavioral Manipulations

III. Concluding Remarks


Author Index

Subject Index


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© Academic Press 1978
Academic Press
eBook ISBN:

About the Editor

Richard T. Robertson

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