Neuroanatomical Research Techniques

Neuroanatomical Research Techniques

1st Edition - January 1, 1978

Write a review

  • Editor: Richard T. Robertson
  • eBook ISBN: 9781483266404

Purchase options

Purchase options
DRM-free (PDF)
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order


Neuroanatomical Research Techniques discusses developments in major neuroanatomical research techniques. The book is organized into four parts. Part I deals generally with the preparation and study of brain tissue. It includes a chapter on the microscope, discussing optical magnification, limitations of microscopy, and optical contrasting methods. Other chapters summarize basic techniques for tissue preparation and sectioning; present guidelines for a number of standard, but essential, staining procedures; and present sophisticated and contemporary computer techniques that are proving to be invaluable as neuroanatomy evolves from a qualitative to a quantitative discipline. Part II deals with techniques often used for the study of normal tissue. These include the Golgi method, fluorescence histochemistry, techniques for staining single neurons, and the use of the electron microscope. Part III presents techniques for studying intrinsic connections of the nervous system. These include techniques for silver impregnation of degenerating fibers; autoradiographic technique for studying axonal projections; and somatopetal movement of horseradish peroxidase as a tool for studying connections and neuron morphology. Part IV discusses the interpretation of results from neuroanatomical research techniques and presents examples of the applications of neuroanatomical methods to major problems in physiological psychology.

Table of Contents

  • List of Contributors



    Preparation and Study of Brain Tissues

    Chapter 1 Microscopy and Photomicrography: an Introduction

    I. The Use of the Microscope

    II. Photographic Techniques

    Chapter 2 Tissue Preparation and Basic Staining Techniques

    I. Introduction

    II. Tissue Fixation

    III. Preparation of Tissues for Sectioning

    IV. Mounting of Sections

    V. Staining Procedures

    VI. Conclusions


    Chapter 3 Computer-Assisted Recording of Neuroanatomical Data

    I. Introduction

    II. Suitability of Computers to Recording Neuroanatomical Data

    III. Methodology

    IV. Appearance of the Data, Analysis, and Interpretation

    V. Technical Problems, Methodological Limitations, and Interpretive Difficulties

    VI. Summary and Comments


    Techniques for the Study of Normal Tissue

    Chapter 4 The Methods of Golgi

    I. Introduction

    II. Why Use the Golgi Methods?

    III. Where is the Golgi Useful?

    IV. How to Use the Golgi Methods

    V. What are the Artifacts and Pitfalls?

    VI. Rationale of the Golgi Methods

    VII. Interpretation of the Data


    Chapter 5 Fluorescence Histochemistry

    I. Introduction

    II. Fluorescence Histochemical Methods for Demonstrating Biogenic Amines in Neurons

    III. Conclusions


    Chapter 6 Single-Cell Staining Techniques

    I. Introduction

    II. Microelectrode Injection

    III. Axonal Iontophoresis: Procedure

    IV. Tissue Processing for Light Microscopy

    V. Ultrastructural Use of Intracellular Dyes

    VI. Possible Limitations of Intracellular Staining

    VII. Prospectus

    VIII. Addendum


    Chapter 7 Electron Microscopy and the Study of the Ultrastructure of the Central Nervous System

    I. Introduction

    II. Specimen Preparation

    III. Additional Selective Methods for Electron Microscopy

    IV. Ultrastructure: The Interpretation of the Electron Microscopic Image


    Study of Connections in the Nervous System

    A. Techniques Based on Orthograde Processes

    Chapter 8 The Study of Degenerating Nerve Fibers Using Silver-Impregnation Methods

    Chapter 9 The Use of Axonal Transport for Autoradiographic Tracing of Pathways in the Central Nervous System

    Chapter 10 The Use of Somatofugal Transport of Horseradish Peroxidase for Tract Tracing and Cell Labeling

    Chapter 11 Neurochemical Effects of Lesions

    B. Techniques Based on Retrograde Processes

    Chapter 12 Analysis of Retrograde Degeneration in Cell Soma following Axon Transection

    Chapter 13 A Review of the Retrograde Transport Technique

    C. Electrophysiological Techniques

    Chapter 14 Electrophysiological Mapping Techniques


    Chapter 15 Histological Techniques in Neuropsychology: The Past and Some Trends for the Future

    I. Introduction

    II. Some Examples of Experiments with Simultaneous Anatomical and Behavioral Manipulations

    III. Concluding Remarks


    Author Index

    Subject Index

Product details

  • No. of pages: 462
  • Language: English
  • Copyright: © Academic Press 1978
  • Published: January 1, 1978
  • Imprint: Academic Press
  • eBook ISBN: 9781483266404

About the Editor

Richard T. Robertson

Ratings and Reviews

Write a review

There are currently no reviews for "Neuroanatomical Research Techniques"