Description

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The third of 3 volumes covering Natural product biosynthesis by microorganisms and plants.

Key Features

  • This new volume continues the legacy of this premier serial
  • Contains quality chapters authored by leaders in the field
  • The third of 3 volumes, it has chapters on such topics as metabolic pathways in Aspergillus oryzae, heterologous gene clusters and cyanobacteria as a source of natural products

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

Contributors

Preface

Methods in Enzymology

Chapter One. Methods for the Study of Endophytic Microorganisms from Traditional Chinese Medicine Plants

1 Introduction

2 Experimental Methods

3 Novel Bioactive Compounds from Endophytic Actinobacteria

4 Conclusion

References

Chapter Two. Cyanobacteria as a Source of Natural Products

1 Introduction

2 Cyanobacteria as Starting Material for Natural Products

3 Screening for New Compounds and Producers

4 Genomic Mining of Cyanobacterial Natural Products

5 Summary

References

Chapter Three. Isolating Antifungals from Fungus-Growing Ant Symbionts Using a Genome-Guided Chemistry Approach

1 Introduction

2 Strain Isolation and Genome Sequencing

3 Identifying Antifungals made by Pseudonocardia P1

4 Identifying Antifungals made by S. albus S4

5 Summary and Perspectives

References

Chapter Four. Gamma-Butyrolactone and Furan Signaling Systems in Streptomyces

1 Introduction

2 AfsA-Like Enzymes and the Biosynthesis of Signaling Molecules

3 Heterologous Expression of an AfsA-Like-Containing Mini-Cluster Combined with Comparative Metabolic Profiling

4 Genetic Disruption of mmfLHP and Chemical Complementation Experiments

5 Conclusion

References

Chapter Five. Old Meets New: Using Interspecies Interactions to Detect Secondary Metabolite Production in Actinomycetes

1 Introduction

2 Setting Up Binary Actinomycete Interactions

3 Activity-Guided Fractionation for Amychelin Isolation

4 Determining the Affinity of Amychelin for Fe

5 Identifying the Amychelin Biosynthetic Gene Cluster

6 Transcriptomic Analysis using Nanostring Technology

7 Summary

References

Chapter Six. Finding and Analyzing Plant Metabolic Gene Clusters

1 Introduc

Details

No. of pages:
512
Language:
English
Copyright:
© 2012
Published:
Imprint:
Academic Press
eBook ISBN:
9780124046177
Print ISBN:
9780124046344

About the serial-volume-editor

David Hopwood

Affiliations and Expertise

John Innes Centre and AFRC Institute of Plant Science Research, Norwich, UK