This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The second of 3 volumes covering Natural product biosynthesis by microorganisms and plants.

Key Features

  • This new volume continues the legacy of this premier serial
  • Contains quality chapters authored by leaders in the field
  • The second of 3 volumes it has chapters on such topics as biological chlorination, bromination and iodination, and phylogenetic approaches to natural product structure prediction


Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

Chapter One. In Vivo Production of Thiopeptide Variants

1 Introduction

2 Generation of Thiopeptide Variants


Chapter Two. Microviridin Biosynthesis

1 Introduction

2 Reconstitution In Vitro of the Cyclization Reactions of the Microviridin Biosynthetic Pathway from P. agardhii

3 Influence of Leader Peptide on the Cyclization Reactions

4 Summary


Chapter Three. Cyclotide Isolation and Characterization

1 Introduction

2 Isolation of Cyclotides from Plant Tissues

3 Isolation of Nucleic Acids Encoding Cyclotide Precursors

4 Mass Spectrometric Detection and Characterization of Cyclotides

5 Structural Analysis of Cyclotides

6 Membranolytic Assays of Cyclotides

7 Summary


Chapter Four. Ribosomally Encoded Cyclic Peptide Toxins from Mushrooms

1 Introduction

2 Detection and Purification of Amanita Cyclic Peptide Toxins

3 Identification of Cyclic Peptide Genes in Amanita and Other Fungi

4 Purification and Assay of Prolyl Oligopeptidase (POP) from Mushrooms

5 Immunodetection of POP in Mushroom Tissues

6 Summary


Chapter Five. The Pictet–Spengler Mechanism Involved in the Biosynthesis of Tetrahydroisoquinoline Antitumor Antibiotics

1 Introduction

2 Biosynthetic Analysis of THIQ Core Scaffold Assembly

3 Reaction Mechanism for Constructing a Pentacyclic THIQ Core Skeleton Catalyzed by a Novel Function for a Single NRPS Module

4 Comparison Between Typical Peptide Synthesis and the Pictet–Spengler Reaction Catalyzed by NRPS Machinery

5 Reaction Profiles Affected by the Chain Length of the Acyl Moiety

6 Multiple Roles for the Fatty Acyl Chain During the Maturation Process in Late-Stage Biosynthesis

7 Methods for Substrate Synthesis, Enzyme Preparation, and Assay Co


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© 2012
Academic Press
eBook ISBN:
Print ISBN:

About the serial-volume-editor

David Hopwood

Affiliations and Expertise

John Innes Centre and AFRC Institute of Plant Science Research, Norwich, UK