This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The second of 3 volumes covering Natural product biosynthesis by microorganisms and plants.

Key Features

  • This new volume continues the legacy of this premier serial
  • Contains quality chapters authored by leaders in the field
  • The second of 3 volumes it has chapters on such topics as biological chlorination, bromination and iodination, and phylogenetic approaches to natural product structure prediction
  • Readership

    Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

    Table of Contents

    Chapter One. In Vivo Production of Thiopeptide Variants

    1 Introduction

    2 Generation of Thiopeptide Variants


    Chapter Two. Microviridin Biosynthesis

    1 Introduction

    2 Reconstitution In Vitro of the Cyclization Reactions of the Microviridin Biosynthetic Pathway from P. agardhii

    3 Influence of Leader Peptide on the Cyclization Reactions

    4 Summary


    Chapter Three. Cyclotide Isolation and Characterization

    1 Introduction

    2 Isolation of Cyclotides from Plant Tissues

    3 Isolation of Nucleic Acids Encoding Cyclotide Precursors

    4 Mass Spectrometric Detection and Characterization of Cyclotides

    5 Structural Analysis of Cyclotides

    6 Membranolytic Assays of Cyclotides

    7 Summary


    Chapter Four. Ribosomally Encoded Cyclic Peptide Toxins from Mushrooms

    1 Introduction

    2 Detection and Purification of Amanita Cyclic Peptide Toxins

    3 Identification of Cyclic Peptide Genes in Amanita and Other Fungi

    4 Purification and Assay of Prolyl Oligopeptidase (POP) from Mushrooms

    5 Immunodetection of POP in Mushroom Tissues

    6 Summary


    Chapter Five. The Pictet–Spengler Mechanism Involved in the Biosynthesis of Tetrahydroisoquinoline Antitumor Antibiotics

    1 Introduction

    2 Biosynthetic Analysis of THIQ Core Scaffold Assembly

    3 Reaction Mechanism for Constructing a Pentacyclic THIQ Core Skeleton Catalyzed by a Novel Function for a Single NRPS Module

    4 Comparison Between Typical Peptide Synthesis and the Pictet–Spengler Reaction Catalyzed by NRPS Machinery

    5 Reaction Profiles Affected by the Chain Length of the Acyl Moiety

    6 Multiple Roles for the Fatty Acyl Chain During the Maturation Process in Late-Stage Biosynthesis

    7 Methods for Substrate Synthesis, Enzyme Preparation, and Assay Co


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    © 2012
    Academic Press
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