Natural Product Biosynthesis by Microorganisms and Plants Part B, Volume 516

1st Edition

Serial Volume Editors: David Hopwood
Hardcover ISBN: 9780123942913
eBook ISBN: 9780123946287
Imprint: Academic Press
Published Date: 17th October 2012
Page Count: 462
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Table of Contents

Chapter One. In Vivo Production of Thiopeptide Variants

1 Introduction

2 Generation of Thiopeptide Variants

References

Chapter Two. Microviridin Biosynthesis

1 Introduction

2 Reconstitution In Vitro of the Cyclization Reactions of the Microviridin Biosynthetic Pathway from P. agardhii

3 Influence of Leader Peptide on the Cyclization Reactions

4 Summary

References

Chapter Three. Cyclotide Isolation and Characterization

1 Introduction

2 Isolation of Cyclotides from Plant Tissues

3 Isolation of Nucleic Acids Encoding Cyclotide Precursors

4 Mass Spectrometric Detection and Characterization of Cyclotides

5 Structural Analysis of Cyclotides

6 Membranolytic Assays of Cyclotides

7 Summary

References

Chapter Four. Ribosomally Encoded Cyclic Peptide Toxins from Mushrooms

1 Introduction

2 Detection and Purification of Amanita Cyclic Peptide Toxins

3 Identification of Cyclic Peptide Genes in Amanita and Other Fungi

4 Purification and Assay of Prolyl Oligopeptidase (POP) from Mushrooms

5 Immunodetection of POP in Mushroom Tissues

6 Summary

References

Chapter Five. The Pictet–Spengler Mechanism Involved in the Biosynthesis of Tetrahydroisoquinoline Antitumor Antibiotics

1 Introduction

2 Biosynthetic Analysis of THIQ Core Scaffold Assembly

3 Reaction Mechanism for Constructing a Pentacyclic THIQ Core Skeleton Catalyzed by a Novel Function for a Single NRPS Module

4 Comparison Between Typical Peptide Synthesis and the Pictet–Spengler Reaction Catalyzed by NRPS Machinery

5 Reaction Profiles Affected by the Chain Length of the Acyl Moiety

6 Multiple Roles for the Fatty Acyl Chain During the Maturation Process in Late-Stage Biosynthesis

7 Methods for Substrate Synthesis, Enzyme Preparation, and Assay Co


Description

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The second of 3 volumes covering Natural product biosynthesis by microorganisms and plants.

Key Features

  • This new volume continues the legacy of this premier serial
  • Contains quality chapters authored by leaders in the field
  • The second of 3 volumes it has chapters on such topics as biological chlorination, bromination and iodination, and phylogenetic approaches to natural product structure prediction

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists


Details

No. of pages:
462
Language:
English
Copyright:
© Academic Press 2012
Published:
Imprint:
Academic Press
eBook ISBN:
9780123946287
Hardcover ISBN:
9780123942913

Reviews

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The second of 3 volumes covering Natural product biosynthesis by microorganisms and plants.


About the Serial Volume Editors

David Hopwood Serial Volume Editor

Affiliations and Expertise

John Innes Centre and AFRC Institute of Plant Science Research, Norwich, UK