Description

This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 3 volumes covering Natural product biosynthesis by microorganisms and plants, it has chapters on such topics as Kinetics of plant sesquiterpene synthases, Terpenoid biosynthesis in fungi, and plant Type III polyketide synthases.

Key Features

  • Contains quality chapters authored by leaders in the field
  • The first of 3 volumes
  • Has chapters on such topics as kinetics of plant sesquiterpene synthases, terpenoid biosynthesis in fungi, and plant Type III polyketide synthases

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • Series Page
  • Contributors
  • Preface
  • Methods in Enzymology
  • Chapter One Steady-State Kinetic Characterization of Sesquiterpene Synthases by Gas Chromatography–Mass Spectroscopy
    • 1 Introduction
    • 2 Experimental Components and Considerations
    • 3 Pilot Experiments
    • 4 Steady-State Kinetic Experiments
    • 5 Data Handling/Processing
    • 6 Summary
  • Chapter Two Automating Gene Library Synthesis by Structure-Based Combinatorial Protein Engineering
    • 1 Overview
    • 2 Plasmid Library Synthesis
    • 3 Optimization of SCOPE PCR Conditions
    • 4 Generic SCOPE Method
    • 5 Applications of SCOPE Synthesis
    • 6 Troubleshooting
    • 7 Conclusions
  • Chapter Three In Planta Transient Expression Analysis of Monoterpene Synthases
    • 1 Introduction
    • 2 Plant Transformation Vector Construction
    • 3 Agrobacterium-Mediated Transient Expression
    • 4 Agrobacterium Preparation for High-Throughput Analysis
    • 5 Product Identification
    • 6 Transient Expression of a Kiwifruit Linalool Synthase
    • 7 Conclusions
  • Chapter Four Natural Rubber Biosynthesis in Plants
    • 1 Introduction
    • 2 Preparing Samples for Assaying Rubber Transferase Activity
    • 3 The Rubber Transferase Assay
    • 4 Identification and Purification of Rubber Transferase
    • 5 Qualitative Protein Analysis
    • 6 Protein Quantification
    • 7 Summary
  • Chapter Five Discovery and Characterization of Terpenoid Biosynthetic Pathways of Fungi
    • 1 Introduction
    • 2 Identification, Cloning, and Characterization of Terpene Synthases
    • 3 Structural Characterization and Engineering of Fungal Terpene Synthase Activities
    • 4 Identification and Characterization of Biosynthetic Gene Clusters
    • 5 Genome Mi

Details

No. of pages:
472
Language:
English
Copyright:
© 2012
Published:
Imprint:
Academic Press
Electronic ISBN:
9780123946270
Print ISBN:
9780123942906

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