This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 3 volumes covering Natural product biosynthesis by microorganisms and plants, it has chapters on such topics as Kinetics of plant sesquiterpene synthases, Terpenoid biosynthesis in fungi, and plant Type III polyketide synthases.

Key Features

  • Contains quality chapters authored by leaders in the field
  • The first of 3 volumes
  • Has chapters on such topics as kinetics of plant sesquiterpene synthases, terpenoid biosynthesis in fungi, and plant Type III polyketide synthases
  • Readership

    Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

    Table of Contents

    1. Steady-state kinetic characterization of sesquiterpene synthases by gas chromatography-mass spectroscopy
      Steven R. Garrett, Richard J. Morris, and Paul E. O’Maille
    2. Automating gene library synthesis by structure-based combinatorial protein engineering (SCOPE): examples from plant sesquiterpene synthases
      Melissa Dokarry, Caroline Laurendon, Paul E. O’Maille
    3. In planta Transient Expression Analysis of Monoterpene Synthases
      Sol A. Green, Xiuyin Chen and Adam J. Matich
    4. Natural Rubber Biosynthesis in Plants: Rubber Transferase
      Katrina Cornish and Wenshuang Xie
    5. Discovery and Characterization of Terpenoid Biosynthetic Pathways of Fungi
      Grayson T. Wawrzyn, Sarah E. Bloch and Claudia Schmidt-Dannert
    6. Menaquinone biosyntheses in microorganisms
      Tohru Dairi
    7. Diversity and analysis of bacterial terpene synthases
      Yuuki Yamada, David E. Cane and Haruo Ikeda
    8. Platensimycin and platencin biosynthesis in Streptomyces platensis, showcasing discovery and characterization of novel bacterial diterpene synthases
      Michael J. Smanski, Ryan M. Peterson, and Ben Shen
    9. Strategies for Engineering Plant Natural Products:The Iridoid-Derived Monoterpene Indole Alkaloids of Catharanthus roseus
      Sarah O'Connor
    10. Discovery and functional analysis of monoterpenoid indole alkaloid pathways in plants
      Vincenzo De Luca, Vonny Salim, Dylan Levac1, Sayaka Masada Atsumi and Fang Yu
    11. Biochemical Genomics for Gene Discovery in Benzylisoquinoline Alkaloid Biosynthesis in Opium Poppy and Related Species
      Thu Thuy T. Dang, Akpevwe Onoyovwi, Scott C. Farrow and Peter J. Facchini
    12. Analysis and modification of ergot alkaloid pro


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    © 2012
    Academic Press
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