NanoArmoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes

NanoArmoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes

1st Edition - April 13, 2017
This is the Latest Edition
  • Editor: C. Vijay Kumar
  • eBook ISBN: 9780128105030
  • Hardcover ISBN: 9780128105023

Purchase options

Purchase options
DRM-free (Mobi, EPub, PDF)
Available
Sales tax will be calculated at check-out

Institutional Subscription

Free Global Shipping
No minimum order

Description

Nanoarmoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes, Volume 590 is the latest volume in the Methods in Enzymology series that focuses on nanoarmoring of enzymes and the rational design of polymer-wrapped enzymes. This new volume presents the most updated information on a variety of topics, including specific chapters on Encapsulating Proteins in Nanoparticles: Batch by Batch or One by One, Enzyme Adsorption on Nanoparticle Surfaces Probed by Highly Sensitive Second Harmonic Light Scattering, Armoring Enzymes by Metal–Organic Frameworks by the Coprecipitation Method, and Enzyme Armoring by an Organosilica Layer: Synthesis and Characterization of Hybrid Organic/Inorganic Nanobiocatalysts. Users will find this to be an all-encompassing resource on nanoarmoring in enzymes.

Key Features

  • Focuses on the nanoarmoring of enzymes
  • Covers the rational design of polymer-wrapped enzymes
  • Includes contributions from leading authorities working in enzymology
  • Informs and updates on all the latest developments in the field of enzymology

Readership

Biochemists, biophysicists, molecular biologists, analytical chemists, and physiologists

Table of Contents

  • Chapter One: Encapsulating Proteins in Nanoparticles: Batch by Batch or One by One
    Y. Liu and A. Cao

    • Abstract
    • 1 Introduction
    • 2 “Batch-by-Batch” Encapsulation of Proteins in Silica NPs
    • 3 “One-by-One” Encapsulation of Proteins in NPs
    • 4 Summary and Outlook
    • Acknowledgments

    Chapter Two: Enzyme Adsorption on Nanoparticle Surface Probed by Highly Sensitive Second Harmonic Light Scattering
    A. Das, A. Chakrabarti and P.K. Das

    • Abstract
    • 1 Introduction
    • 2 Techniques for Probing Nanoparticle–Protein Interaction
    • 3 Second Harmonic Light Scattering
    • 4 Sample Preparation
    • 5 Interaction of ADH With GNPs
    • 6 Interaction of Insulin With GNPs
    • 7 Stoichiometry of the Protein–GNP Conjugate
    • 8 Summary and Future Outlook
    • Acknowledgment

    Chapter Three: Armoring Enzymes by Metal–Organic Frameworks by the Coprecipitation Method
    M. Hou and J. Ge

    • Abstract
    • 1 Introduction
    • 2 Equipment
    • 3 Materials
    • 4 Protocol
    • 5 Safety
    • 6 Step1: Preparation of Enzyme@MOF
    • 7 Step2: Preparation of Buffers
    • 8 Step3: Purification of the Product
    • 9 Step4: Characterization of Enzyme@MOF
    • 10 Step5: Measurement of Enzyme Loading in MOFS
    • 11 Step6: Analysis of the Location of Enzymes in MOFS
    • 12 Step7: Determination of Enzyme Activities
    • 13 Conclusions

    Chapter Four: Enzyme Armoring by an Organosilica Layer: Synthesis and Characterization of Hybrid Organic/Inorganic Nanobiocatalysts
    M.R. Correro, S. Sykora, P.F.-X. Corvini and P. Shahgaldian

    • Abstract
    • 1 Introduction
    • 2 Equipments and Chemicals
    • 3 Safety Guidelines
    • 4 Procedures
    • 5 Conclusions
    • Acknowledgments

    Chapter Five: Strategies for Biophysical Characterization of Protein–Polymer Conjugates
    C. Williams, M.L. Dougherty, K. Makaroff, J. Stapleton, D. Konkolewicz, J.A. Berberich and R.C. Page

    • Abstract
    • 1 Introduction
    • 2 Biophysical Methods
    • 3 Conclusions
    • Acknowledgments

    Chapter Six: Guide to the Preparation of Molecularly Imprinted Polymer Nanoparticles for Protein Recognition by Solid-Phase Synthesis
    J. Xu, P.X. Medina-Rangel, K. Haupt and B.Tse Sum Bui

    • Abstract
    • 1 Introduction
    • 2 Protocols
    • 3 Physicochemical Characterization of MIP-NPs
    • 4 Evaluation of the Binding Characteristics of MIP-NPs Using a Quartz Crystal Microbalance
    • 5 MIP-NPs Protect Enzyme From Thermal and pH Denaturation
    • 6 Concluding Remarks
    • 7 Notes
    • Acknowledgments

    Chapter Seven: Armored Urease: Enzyme-Bioconjugated Poly(acrylamide) Hydrogel as a Storage and Sensing Platform
    K.R. Kunduru, S.N.R. Kutcherlapati, D. Arunbabu and T. Jana

    • Abstract
    • 1 Introduction
    • 2 Reversible Immobilization
    • 3 Jack Bean Urease: Active Site
    • 4 Immobilization of Jack Bean Urease
    • 5 Methodology
    • 6 Results and Discussion
    • 7 Conclusions and Future Perspectives
    • Acknowledgments

    Chapter Eight: Armored Enzyme–Nanohybrids and Their Catalytic Function Under Challenging Conditions
    O.V. Zore, R.M Kasi and C.V. Kumar

    • Abstract
    • 1 Introduction
    • 2 Methods: Synthesis of Bienzyme–Polymer Conjugates
    • 3 Methods: Synthesis of Bienzyme–Polymer–Graphene Oxide (GOx–HRP–PAA/GO) Hybrid Materials
    • 4 Characterization
    • 5 Conclusions
    • Acknowledgments

    Chapter Nine: Approaches for Conjugating Tailor-Made Polymers to Proteins
    M. Paeth, J. Stapleton, M.L. Dougherty, H. Fischesser, J. Shepherd, M. McCauley, R. Falatach, R.C. Page, J.A. Berberich and D. Konkolewicz

    • Abstract
    • 1 Introduction
    • 2 Polymer Synthetic Procedures
    • 3 Protein Conjugation Methods—Amine Based
    • 4 Polymer Conjugation by GT Using Click Approaches
    • 5 Conjugate Characterization Methods
    • 6 Conclusions

    Chapter Ten: NanoArmoring of Enzymes by Polymer-Functionalized Iron Oxide Nanoparticles
    G. Premaratne, L. Coats and S. Krishnan

    • Abstract
    • 1 Introduction
    • 2 NanoArmoring by Making Polymer–Enzyme Conjugates
    • 3 MNP–Polymer–Enzyme Conjugates
    • 4 Covalent vs Noncovalent Immobilization of Peroxidase Active Proteins on PolyMNPs. What Difference Does It Make in the Electrocatalytic Activity and Kinetics?
    • 5 Effect of MNP Size on Activity and Recovery of GOx (Park, McConnell, Boddohi, Kipper, & Johnson, 2011)
    • 6 Summary
    • 7 Future Outlook
    • Acknowledgments

    Chapter Eleven: Expression of Cellulolytic Enzyme as a Fusion Protein That Reacts Specifically With a Polymeric Scaffold
    P. Katyal, Y. Yang, O. Vinogradova and Y. Lin

    • Abstract
    • 1 Introduction
    • 2 Materials
    • 3 Methods
    • 4 Future Outlook
    • Acknowledgments

    Chapter Twelve: Nanoarmoring of Proteins by Conjugation to Block Copolymer Micelles
    N. Suthiwangcharoen and R. Nagarajan

    • Abstract
    • 1 Introduction
    • 2 Synthesis of Block Copolymer–Protein (BSA) Conjugate
    • 3 Characterization of Block Copolymer–Protein Conjugate
    • 4 Characterization of Protein–Polymer Conjugate Micelle
    • 5 Conclusions
    • Acknowledgments

    Chapter Thirteen: Semisynthetic Enzymes by Protein–Peptide Site-Directed Covalent Conjugation: Methods and Applications
    J.M Palomo

    • Abstract
    • 1 Introduction
    • 2 Protein–Peptide Site-Directed Covalent Conjugation
    • 3 The New Semisynthetic Lipases in Biotransformations
    • 4 Conclusions
    • Acknowledgments

    Chapter Fourteen: Transgultaminase-Mediated Nanoarmoring of Enzymes by PEGylation
    A. Grigoletto, A. Mero, K. Maso and G. Pasut

    • Abstract
    • 1 Introduction
    • 2 Materials and Reagents
    • 3 Methods

    Chapter Fifteen: Polymer-Based Protein Engineering: Synthesis and Characterization of Armored, High Graft Density Polymer–Protein Conjugates
    S. Carmali, H. Murata, C. Cummings, K. Matyjaszewski and A.J. Russell

    • Abstract
    • 1 Introduction
    • 2 Protocols
    • 3 Protein–Polymer-Based Engineering
    • 4 Conclusions
    • Acknowledgment

    Chapter Sixteen: Nano-Armoring of Enzymes: Rational Design of Polymer-Wrapped Enzymes
    K. Raghupathi and S. Thayumanavan

    • Abstract
    • 1 Introduction
    • 2 Covalent Conjugation
    • 3 Electrostatic Complexation
    • 4 Noncovalent Entrapment
    • 5 Conclusions
    • Acknowledgments

    Chapter Seventeen: Nanoarmored Enzymes for Organic Enzymology: Synthesis and Characterization of Poly(2-Alkyloxazoline)–Enzyme Conjugates
    M. Leurs and J.C. Tiller

    • Abstract
    • 1 Introduction
    • 2 Synthesis of Poly(2-Alkyloxazoline)s With an NH2 End Group
    • 3 Conjugation of Enzymes With Poly(2-Alkyloxazoline)s
    • 4 Conjugate Characterization
    • 5 Activity Evaluation of Poly(2-Alkyloxazoline)–Enzyme Conjugates
    • 6 Conclusions
    • Acknowledgments

    Chapter Eighteen: Preparation and Applications of Dendronized Polymer–Enzyme Conjugates
    A. Küchler, D. Messmer, A.D. Schlüter and P. Walde

    • Abstract
    • 1 Introduction
    • 2 The Denpol de-PG2
    • 3 The Denpol–Enzyme Conjugate de-PG2-BAH-proK
    • 4 Synthesis and Characterization of Denpols Carrying Other Types of Enzymes
    • 5 Immobilization of de-PG2-BAH-proK on Silicate Surfaces
    • 6 Entrapment of Denpol–Enzyme Conjugates Inside Phospholipid Vesicles
    • 7 Conclusions and Outlook
    • Acknowledgments

    Chapter Nineteen: Nanoarmoring of Enzymes by Interlocking in Cellulose Fibers With Poly(Acrylic Acid)
    C.M. Riccardi, R.M. Kasi and C.V. Kumar

    • Abstract
    • 1 Introduction
    • 2 Equipment and Reagents
    • 3 Methods
    • 4 Conclusions
    • Acknowledgments

Product details

  • No. of pages: 558
  • Language: English
  • Copyright: © Academic Press 2017
  • Published: April 13, 2017
  • Imprint: Academic Press
  • eBook ISBN: 9780128105030
  • Hardcover ISBN: 9780128105023

About the Serial Volume Editor

C. Vijay Kumar

Department of Chemistry, University of Connecticut, USA

Affiliations and Expertise

Department of Chemistry, University of Connecticut, USA