Mutagenicity: Assays and Applications - 1st Edition - ISBN: 9780128092521

Mutagenicity: Assays and Applications

1st Edition

Editors: Ashutosh Kumar Vasily Dobrovolsky Alok Dhawan Rishi Shanker
Paperback ISBN: 9780128092521
Imprint: Academic Press
Published Date: 1st October 2017
Page Count: 256
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Mutagenicity: Assays and Applications presents an extensive examination of the detection, assessment and future of mutagenicity, particularly as it concerns human health and the environment. Chapters focused on specific types of mutagens or testing methods for their detection collectively explore the current state of human and environmental mutagenesis, future perspectives and regulatory needs. The test procedures for measuring mutagenicity, their advantages and limitations are described with practical and procedural detail, along with their presentation and data processing aspects. It is an essential reference covering the breadth and depth of the field of mutagenicity studies and regulation.

By providing both important introductory material and practical assays and applications, this book is useful to graduate students, academic and industry researchers and regulators at various stages of their careers, leading to improved risk assessment and regulation.

Key Features

  • Presents an up-to-date and in-depth review of the current state of mutagenesis research
  • Draws upon the combined experience and expertise of an international group of highly respected editors and chapter authors
  • Provides an introduction to the concept of mutagenesis with particular consideration given to novel chemicals and materials


Academic and industry researchers, regulators and graduate students in toxicology

Table of Contents

  1. Mutagenesis and health problems
    2. Molecular mechanisms of mutagenesis
    3. Bacterial mutation assays
    4. Gene mutation assays in cultured mammalian cells
    5. Chromosomal aberration test
    6. In vivo cytogenetic assay
    7. Mutagenicity testing in environmental pollution control
    8. Mutagens in food and water
    9. Emerging methods for the identification of mutagens
    10. Guidelines and registration of the new compounds or drug like molecules
    11. Mutagenicity testing, regulatory guidelines and current needs


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Academic Press
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About the Editor

Ashutosh Kumar

Dr. Ashutosh Kumar works as an Assistant Professor at the Division of Biological & Life Sciences, Ahmedabad University, Gujarat, India from June 2012. Before this, he worked in CSIR-Indian Institute of Toxicology Research, Lucknow as a research fellow and has been involved in understanding the adverse effects of nanomaterials on biological systems. His research areas of interest are microbiology and biotechnology with special reference to nanomaterials. He established several new methods for nanomaterial toxicology in India. He developed a novel method for the detection of uptake of nanoparticles in live bacteria for several generations. He also unravelled the mechanism of metal oxide nanoparticles induced genotoxicity and apoptosis in the prokaryotic as well as in eukaryotic cells using the state of art techniques such as flow cytometry, electron and confocal microscopy, biochemical enzyme assays, genomics and proteomics. The work conducted by him is in the frontiers of nanomaterial safety area worldwide. His studies shall have far reaching applications in predicting the adverse health effects of nanoparticles and safe use of ENMs to protect human and environmental health. He has received several national and international awards including the “INSA MEDAL FOR YOUNG SCIENTIST 2014” in the area of Health Sciences for his scientific contributions. His research work has been published in reputed journals such as Free Radical Biology and Medicine, Nanomedicine, Cytometry A, Chemosphere, Journal of Biological Chemistry, Journal of Biomedical Nanotechnology, Nanotoxicology, Toxicological Sciences (cover page article) and others. His teaching interests include Biotechnology, Genomics Proteomics and Metabolomics, Biophysical Instrumentation, Genetic engineering, Microbiology, Cell and Molecular Biology, Genetics and Animal Biotechnology.

Affiliations and Expertise

Assistant Professor, Institute of Life Sciences, Ahmedabad University, Ahmedabad, India

Vasily Dobrovolsky

Vasily Dobrovolsky joined the U.S. FDA’s National Center for Toxicological Research (NCTR) in 1994, first as a postdoctoral fellow from the Oak Ridge Institute for Science and Education (ORISE), then as a staff fellow in 1998 and later as Research Microbiologist at the Division of Genetic and Molecular Toxicology. He oversees a group consisting of NCTR scientists, students and support personnel who are involved in research projects aimed at developing novel methodologies for basic and applied genetic toxicology. Vasily received his MS degree in Biotechnology in 1988 from Moscow Institute of Physics and Technology (Moscow, Russia) and PhD degree in Molecular Biology in 1994 from the Institute of Bioorganic Chemistry (Russian Academy of Sciences). This was followed by postdoctoral training at NCTR under the guidance from Dr. Robert H. Heflich, designing in vitro and in vivo models for detecting mutation in endogenous and transgenic reporter genes. Dr. Dobrovolsky has published over 60 journal articles, book chapters, and conference proceedings over his career. He is a member of US EMGS; he has served as a Guest Editor to Special issues of Environmental and Molecular Mutagenesis and Mutagenesis. He participated in working groups from ILSI-HESI, and IWGT interested in integrating genetox assays into general toxicology protocols, quantitative analysis of genetic toxicology data and the Pig-a in vivo gene mutation assay. He was honored to receive an informal Pig-a Award (pink glass pig) in 2015 for his work on sequencing Pig-a mutants.

Affiliations and Expertise

U.S. Food and Drug Administration/National Center for Toxicological Research, Division of Genetic and Molecular Toxicology

Alok Dhawan

Professor Alok Dhawan is currently Director, Institute of Life Sciences, Ahmedabad University, Gujarat on lien from CSIR-Indian Institute of Toxicology Research, Lucknow where he is Principal Scientist and Area Coordinator, Nanomaterial Toxicology Group. He obtained his Ph.D. Biochemistry from University of Lucknow, India in 1991. Professor Dhawan started the area of nanomaterial toxicology in the country and published a guidance document on the safe use of nanomaterials. His group elucidated the mechanism of toxicity of metal oxide nanoparticles in human and bacterial cells. His work has been widely cited. He set up a state of the art nanomaterial toxicology facility at CSIR-IITR. He pioneered the identification of peripheral biomarkers of exposure, effect and susceptibility to xenobiotics. Using DNA damage in lymphocytes as a biomarker of exposure, he has established the reference values for DNA damage in healthy Indian population. He also developed and validated Drosophila melanogaster for in vivo genotoxicity assessment. These can have far reaching applications in predicting the adverse health effects of drugs and chemicals on human health. He was instrumental in promoting the cause of alternate to animal models in toxicology including in silico models. Professor Dhawan has won several honours and awards including the INSA Young Scientist Medal in 1994, CSIR Young Scientist Award in 1999 the Shakuntala Amir Chand Prize of ICMR in 2002 and the Vigyan Ratna by the Council of Science and Technology, UP in 2011. His work in the area of nanomaterial toxicology has won him international accolades as well and he was awarded two Indo-UK projects under the prestigious UK-IERI programme. He also has two European Union Projects under the FP7 and New INDIGO programmes. He founded the Indian Nanoscience Society in 2007. In recognition of his work he has been elected Fellow, The National Academy of Sciences, India; Fellow, The Academy of Toxicological Sciences, USA; Fellow, The Academy of Environmental Biology; Fellow, Academy of science for Animal Welfare; Fellow—Society of Toxicology (India), Founder Fellow, Indian Nanoscience Society; Vice President—Environmental Mutagen Society of India (2006–07); Member—National Academy of Medical Sciences. He has to his credit over 100 publications in peer reviewed international journals. Five reviews/book chapters. Four patents, one copyright and the Comet assay website He has also been a Guest Editor of a special issue of the journal Nanotoxicology (Supplement 1, 2008) and Volume 3(1) 2009 published by Taylor and Francis group, UK. He has edited a book along with Prof. Diana Anderson entitled The Comet Assay In Toxicology which has been published by The Royal Society of Chemistry, UK under its series on Issues in Toxicology. He is the Editor-In-Chief, Journal of Translational Toxicology published by American Scientific Publishers, USA and serves as the Member, Editorial Board of Mutagenesis, Mutation Research Reviews and other journal of repute.

Affiliations and Expertise

Director, Institute of Life Sciences, Ahmedabad University, Gujarat, India; Senior Principal Scientist, CSIR-Indian Institute of Toxicology Research

Rishi Shanker

Professor, Institute of Life Sciences, Ahmedabad University, Ahmedabad, India • Formerly Chief Scientist and Area Coordinator of research on environmental and nanomaterial toxicology at CSIR-Indian Institute of Toxicology Research. • Published more than 80 peer-reviewed articles, has an h-index of 13 and has served as a guest editor on several major journals • Founder Fellow of the Indian Nanoscience Society and Society of Toxicology, India and Elected Member of the National Academic of Sciences, Allahabad

Affiliations and Expertise

Professor, Institute of Life Sciences, Ahmedabad University, Ahmedabad, India