Immunohistochemistry is the use of specific antibodies to stain particular molecular species in situ. This technique has allowed the identification of many more cell types than could be visualized by classical histology, particularly in the immune system and among the scattered hormone-secreting cells of the endocrine system, and has the potential to improve diagnosis, prognosis and therapeutic options of cancer.
This book discusses all aspects of immunohistochemistry and in situ hybridization technologies and the important role they play in reaching a cancer diagnosis. It provides step-by-step instructions on the methods of additional molecular technologies such as DNA microarrays, and microdissection, along with the benefits and limitations of each method. The topics of region-specific gene expression, its role in cancer development and the techniques that assist in the understanding of the molecular basis of disease are relevant and necessary in science today.
This book is the second volume of three planned, individually-sold volumes on this topic. Like Volume 1, this book fully explains the principles and applications of modern techniques used in the field of molecular genetics. It will be of particular interest to pathologists and molecular pathologists conducting both academic and/or clinical research.
- The only book available that translates molecular genetics into cancer diagnosis
- The results of each Immunohistochemical and in situ hybridization method are presented in the form of color illustrations
- Methods discussed were either developed or refined by expert contributors in their own laboratories
Graduate and medical students in cancer research, oncology, pathology, biology, immunology, bioinformatics and endocrinology.
Authors and Coauthors of Volume 2 Foreword Preface to Volume 2 Contents of Volume 1 Prologue Selected Definitions I Molecular Pathology 1.1 Laser Capture Microdissection-Microarray Technology: Global mRNA Amplification for Expression Profiling on Laser Capture Cells 1.2 Comparative Genomic Hybridization Analysis Using Metaphase or Microarray Slides 1.3 Microarray Immunoassay of Complex Specimens: Problems and Technologic Challenges 1.4 Comparative Genomic Hybridization 1.5 Microsatellite Instability in Cancer: Assessment by High Resolution Fluorescent Microsatellite Analysis 1.6 The Role of Extreme Phenotype Selection in Cancer Research 1.7 Rolling Circle Amplification 1.8 Direct, in situ Assessment of Telomere Length Variation in Human Cancers and Preneoplastic Lesions 1.9 Clinical Flow Cytometry of Solid Tumors 1.10 Suppression Subtractive Hybridization Technology II Colorectal Carcinoma 127 2.1 Colorectal Carcinoma: An Introduction 2.2 Role of Immunohistochemical Expression of p53 2.3 Applying Tissue Microarray in Rectal Cancer: Immunostaining of Ki-67 and p53 2.4 Role of Immunohistochemical Expression of p21 in Rectal Carcinoma 2.5 Role of p107 Expression in Colorectal Carcinoma 2.6 Expression of Gastric MUC5AC Mucin During Colon Carcinogenesis 2.7 Role of Cyclooxygenase2 Expression in Colorectal Cancer 2.8 Role of Immunohistochemical Expression of Bcl-2 in Colorectal Carcinoma 2.9 Immunohistochemical Detection of CD97 Protein in Colorectal Carcinoma 2.10 Roles of Immunohistochemical Expression of Cyclin A and Cyclin-Dependent Kinase 2 in Colorectal Tumors 2.11 Role of Mismatch Repair Proteins and Microsatellite Instability in Colon Carcinoma 2.12 Role of CD-61 (Beta-3 Integrin) Glycoprotein in Colon Carcinoma 2.13 Immunohistochemical and in situ Hybridization Analysis of Lumican in Colorectal
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- © Academic Press 2005
- 12th January 2005
- Academic Press
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"The impressive list of contributors, form 15 countries, and the extensive coverage in 492 pages (with many micrographs of excellent quality) maintains the high standard established in Volume 1 of the Handbook series. It represents a valuable addition to the available technical literature on human carcinomas. Professor Hayat is again to be congratulated for producing such a useful book. This book should be considered for purchase by medical school librarians and by hospital Pathology/Oncology departments." --MICRON (2005)