Description

mRNA Formation and Function presents a compendium of techniques geared exclusively toward the understanding of RNA metabolism. It will be particularly useful because a number of different organisms and systems are employed.

Key Features

@introbul:Key Features @bul:* Isolation and characterization of specific RNA binding proteins * RNA metabolism and associated regulatory proteins * RNA detection and localization * A genetic approach to RNA function

Readership

Geneticists, biochemists, molecular biologists, cell biologists, and microbiologists.

Table of Contents

C. Sune, P.R. Bohjanen, Y. Liu, M.A. Garcia-Blanco, and S.F. Jamison, The Tat-TAR RNP, a Master Switch That Regulates HIV-1 Gene Expression. C.R. Miller, S.F. Jamison, and M.A. Garcia-Blanco, HeLa Nuclear Extract: Amodified Protocol. J. Valcarcel, C. Martinez, and M.R. Green, Functional Analysis of Splicing Factors and Regulators. J.G. Patton, B.T. Dye, D.C. Barnard, and J.G. McAfee, Identification of pre-mRNA Splicing Factors and Analysis of RNA-Protein Interaction. G. Gilmartin, In Vitro Analysis of Mammalian Cell mRNA 3 Processing. J. Wilusz, Rapid Identification and Cloning of Sequence-Specific RNA Binding Proteins. J.S. Butler, M.W. Briggs, and A. Proweller, Analysis of Polyadenylation Phenotypes in Saccharomyces cerevisiae. P.D. Gershon, Poly(A) Polymerase/cap-Specific 2-O-Methyltransferase from Vaccinia Virus: Expression, Purification, Uses, and Protein-Ligand Interaction Assays. A.N. Hennigan and A. Jacobson, A Genetic Approach to Mapping Coding Region Determinants of mRNA Instability in Yeast. W.F. Marzluff, M.L. Whitfield, Z. Dominski, and Z.-F. Wang, Identification of the Protein Which Interacts with the 3 End of Histone mRNA. M. Holcik and S.A. Liebhaber, Analysis of mRNP Complexes Assembled in Vitro. A. Laird-Offringa, Analysis of RNA-Binding Proteins Using in Vitro Genetics. L.G. Andrews and J.D. Keene, Interactions of Proteins with Specific Sequences in RNA. C. Jain and J.G. Belasco, A Rapid Genetic Method for the Study of RNA Binding Proteins. P. Ansel-McKinney and L. Gehrke, Footprinting RNA-Protein Complexes with Hydroxyl Radicals. H. Ruan, C.Y. Brown, and D.R. Morris, Analysis of

Details

No. of pages:
395
Language:
English
Copyright:
© 1997
Published:
Imprint:
Academic Press
Print ISBN:
9780125875455
Electronic ISBN:
9780080537047

About the author

Joel Richter

Affiliations and Expertise

Worcester Foundation for Biomedical Research, University of Massachusetts Medical Center, Worcester, U.S.A.