mRNA Formation and Function presents a compendium of techniques geared exclusively toward the understanding of RNA metabolism. It will be particularly useful because a number of different organisms and systems are employed.
@introbul:Key Features @bul:* Isolation and characterization of specific RNA binding proteins
- RNA metabolism and associated regulatory proteins
- RNA detection and localization
- A genetic approach to RNA function
Geneticists, biochemists, molecular biologists, cell biologists, and microbiologists.
C. Sune, P.R. Bohjanen, Y. Liu, M.A. Garcia-Blanco, and S.F. Jamison, The Tat-TAR RNP, a Master Switch That Regulates HIV-1 Gene Expression.
C.R. Miller, S.F. Jamison, and M.A. Garcia-Blanco, HeLa Nuclear Extract: Amodified Protocol.
J. Valcarcel, C. Martinez, and M.R. Green, Functional Analysis of Splicing Factors and Regulators.
J.G. Patton, B.T. Dye, D.C. Barnard, and J.G. McAfee, Identification of pre-mRNA Splicing Factors and Analysis of RNA-Protein Interaction.
G. Gilmartin, In Vitro Analysis of Mammalian Cell mRNA 3 Processing.
J. Wilusz, Rapid Identification and Cloning of Sequence-Specific RNA Binding Proteins.
J.S. Butler, M.W. Briggs, and A. Proweller, Analysis of Polyadenylation Phenotypes in Saccharomyces cerevisiae.
P.D. Gershon, Poly(A) Polymerase/cap-Specific 2-O-Methyltransferase from Vaccinia Virus: Expression, Purification, Uses, and Protein-Ligand Interaction Assays.
A.N. Hennigan and A. Jacobson, A Genetic Approach to Mapping Coding Region Determinants of mRNA Instability in Yeast.
W.F. Marzluff, M.L. Whitfield, Z. Dominski, and Z.-F. Wang, Identification of the Protein Which Interacts with the 3 End of Histone mRNA.
M. Holcik and S.A. Liebhaber, Analysis of mRNP Complexes Assembled in Vitro.
A. Laird-Offringa, Analysis of RNA-Binding Proteins Using in Vitro Genetics.
L.G. Andrews and J.D. Keene, Interactions of Proteins with Specific Sequences in RNA.
C. Jain and J.G. Belasco, A Rapid Genetic Method for the Study of RNA Binding Proteins.
P. Ansel-McKinney and L. Gehrke, Footprinting RNA-Protein Complexes with Hydroxyl Radicals.
H. Ruan, C.Y. Brown, and D.R. Morris, Analysis of
- No. of pages:
- © Academic Press 1997
- 2nd October 1997
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
Worcester Foundation for Biomedical Research, University of Massachusetts Medical Center, Worcester, U.S.A.