Chapter 1. General properties and applications of monoclonal antibodies. 2. Assay techniques. 3. Selection of animals in which hybridomas may be generated. 4. Immunization. 5. Cell culture requirements for hybridomas. 6. Fusion procedures. 7. Alternative strategies for the immortalization of antibody producing cells. 8. Selection and cloning. 9. The use of recombinant DNA methods. 10. Antibody production and purification. 11. Characterisation of monoclonal antibodies. 12. Immunosensors. References. Subject Index.