Molecular of Cloning of Recombinant Dna

Molecular of Cloning of Recombinant Dna

1st Edition - January 1, 1977

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  • Editor: W.A. Scott
  • eBook ISBN: 9780323158725

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Mobilization and Reassembly of Genetic Information documents the proceedings of the Miami Winter Symposium, sponsored by the Department of Biochemistry, University of Miami School of Medicine, Miami, Florida, January 1977. This volume is the 13th in the ""Miami Winter Symposia"" series. Topics for the Miami Winter Symposia focus on areas of biochemistry in which recent progress offers new insights into the molecular basis of biological phenomena. The manuscripts presented by researchers at the symposium cover a wide range of topics including DNA cloning and plasmid biology; yeast DNA expression in Escherichia coli; characterization of tetracycline and ampicillin resistant plasmid cloning vehicles; eukaryotic genome organization; bacterial plasmids containing silk gene sequences; DNA cloning in bacteria for the study of immunoglobulin genes; DNA degradation by rat intestinal nucleases; recombination between bacterial plasmids leading to the formation of plasmid multimers; general methods for inserting specific DNA sequences in cloning vehicles; and cloning and characterization of yeast DNA.

Table of Contents

  • Speakers, Chairmen, and Discussants


    The Eighth Feodor Lynen Lecture: Biochemical Pastimes ... and Future Times

    DNA Cloning and Plasmid Biology

    The Expression of Yeast DNA in Escherichia Coli

    Characterization of Tetracycline and Ampicillin Resistant Plasmid Cloning Vehicles

    Eucaryotic Genome Organization: Cloning and Beyond

    Construction and Use of Safer Bacterial Host Strains for Recombinant DNA Research

    The Nitrogen Fixation (nif) Operon of Klebsiella pneumoniae: Cloning nif Genes and the Isolation of nif Control Mutants

    Applications of Bacteriophage λ in Recombinant DNA Research

    Rapid Selection and Screening Methods for the Isolation of Particular Cloned Eukaryotic DNA Sequences

    Bacterial Plasmids Containing Silk Gene Sequences

    An Approach to the Study of Developmentally Regulated Genes

    Use of an EK-2 Vector for the Cloning of DNA from Higher Organisms

    DNA Cloning in Bacteria as a Tool for Study of Immunoglobulin Genes

    Poster Session: Communications

    DNA Degradation by Rat Intestinal Nucleases

    Restriction Analysis and Cloning of DNA from the ILV Gene Cluster of E. coli K12

    The Localization of the Eco R,-Sensitive Sites on the Chromosomes of Different Bacteria

    Repression Control by Homologous and Heterologous Repression System of Different Bacterial Genera, and the Plasmids Obtained from Bacillus subtilis Group

    Recombination Between Bacterial Plasmids Leading to the Formation of Plasmid Multimers

    Plasmids Carrying the λ i434 cro and c11 Genes Express Specific DNA Binding Activity

    The Filamentous Coliphage M13 as a Cloning Vehicle. Insertion of a Hind II Fragment of the Lac Regulatory Region in M13 RF In Vitro

    General Methods for Inserting Specific DNA Sequences in Cloning Vehicles

    Enzymatic Synthesis of Rabbit Globin Genes: Comparison of T4 DNA Polymerase and RNA-Directed DNA Polymerase

    Organization of Bombyx Mori rDNA

    Cloning and Characterization of Yeast DNA

    Transformation Procedure to E. coli χ 1776 Strain

    Restriction Endonucleases: Protection of Particular Recognition Sites with Antibiotics

Product details

  • No. of pages: 262
  • Language: English
  • Copyright: © Academic Press 1977
  • Published: January 1, 1977
  • Imprint: Academic Press
  • eBook ISBN: 9780323158725

About the Editor

W.A. Scott

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