This volume attempts to describe the purification and assay of the diverse group of proteins involved in otoplasmic organization. Some of the more promising attempts to apply techniques of molecular biology, genetics, and molecular cytochemistry to these proteins are discussed.


Biochemists, cell biologists, molecular biologists, developmental biologists, geneticists, and biophysicists.

Table of Contents

Preparation of Myosin- and Actomyosin-Related Proteins: H. Swanljung-Collins and J.H. Collins, Rapid, High-Yield Purification of Intestinal Brush Border Myosin I. T.J. Lynch, H. Brzeska, I.C. Baines, and E.D. Korn, Purification of Myosin I and Myosin I Heavy Chain Kinase from Acanthamoeba castellanii. Q.G. Medley, S.-F. Lee, and G.P. C~acot~aae, Purification and Characterization of Myosin II Heavy Chain Kinase A from Dictyostelium. M.A. Conti and R.S. Adelstein, Purification and Properties of Myosin Light Chain Kinases. L.J. Wuestehube, D.W. Speicher, A. Shariff, and E.J. Luna, F-Actin Affinity Chromatography of Detergent-Solubilized Plasma Membranes: Purification and Initial Characterization of Ponticulin from ~Dictyostelium discoideum. J.R. Glenney, Jr. , Purification of Calpactins I and II and Isolation of N-Terminal Tail of Calpactin I. A.R. Bresnick and J. Condeelis, Isolation of Actin-Binding Proteins from Dictyostelium discoideum. M. Fechheimer and R. Furukawa, Preparation of 30,000-Da Actin Cross-Linking Protein from Dictyostelium discoideum. P.A. Janmey, Polyproline Affinity Method for Purification of Platelet Profilin and Modification with Pyrene-Maleimide. M. Rozycki, C.E. Schutt, and U. Lindberg, Affinity Chromatography-Based Purification of Profilin: Actin. M.R. Bubb and E.D. Korn, Purification of Actobindin from Acanthamoeba castellanii. J.R. Bamburg, L.S. Minamide, T.E. Morgan, S.M. Hayden, K.A. Giuliano, and A. Koffer, Purification and Characterization of Low-Molecular-Weight Actin-Depolymerizing Proteins from Brain and Cultured Cells. J.F. Casella and J.A. Cooper, Purification of Cap Z from Chicken Skeletal Muscle. Preparation of M


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