Molecular Motors and the Cytoskeleton, Volume 196

1st Edition

Serial Volume Editors: Richard Vallee
Editor-in-Chiefs: John Abelson Melvin Simon
Hardcover ISBN: 9780121820978
eBook ISBN: 9780080883113
Imprint: Academic Press
Published Date: 1st March 1991
Page Count: 559


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Table of Contents

Preparation of Myosin- and Actomyosin-Related Proteins:

H. Swanljung-Collins and J.H. Collins, Rapid, High-Yield Purification of Intestinal Brush Border Myosin I.

T.J. Lynch, H. Brzeska, I.C. Baines, and E.D. Korn, Purification of Myosin I and Myosin I Heavy Chain Kinase from Acanthamoeba castellanii.

Q.G. Medley, S.-F. Lee, and G.P. C~acot~aae, Purification and Characterization of Myosin II Heavy Chain Kinase A from Dictyostelium.

M.A. Conti and R.S. Adelstein, Purification and Properties of Myosin Light Chain Kinases.

L.J. Wuestehube, D.W. Speicher, A. Shariff, and E.J. Luna, F-Actin Affinity Chromatography of Detergent-Solubilized Plasma Membranes: Purification and Initial Characterization of Ponticulin from ~Dictyostelium discoideum.

J.R. Glenney, Jr. , Purification of Calpactins I and II and Isolation of N-Terminal Tail of Calpactin I.

A.R. Bresnick and J. Condeelis, Isolation of Actin-Binding Proteins from Dictyostelium discoideum.

M. Fechheimer and R. Furukawa, Preparation of 30,000-Da Actin Cross-Linking Protein from Dictyostelium discoideum.

P.A. Janmey, Polyproline Affinity Method for Purification of Platelet Profilin and Modification with Pyrene-Maleimide.

M. Rozycki, C.E. Schutt, and U. Lindberg, Affinity Chromatography-Based Purification of Profilin: Actin.

M.R. Bubb and E.D. Korn, Purification of Actobindin from Acanthamoeba castellanii.

J.R. Bamburg, L.S. Minamide, T.E. Morgan, S.M. Hayden, K.A. Giuliano, and A. Koffer, Purification and Characterization of Low-Molecular-Weight Actin-Depolymerizing Proteins from Brain and Cultured Cells.

J.F. Casella and J.A. Cooper, Purification of Cap Z from Chicken Skeletal Muscle.

Preparation of M


This volume attempts to describe the purification and assay of the diverse group of proteins involved in otoplasmic organization. Some of the more promising attempts to apply techniques of molecular biology, genetics, and molecular cytochemistry to these proteins are discussed.


Biochemists, cell biologists, molecular biologists, developmental biologists, geneticists, and biophysicists.


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© Academic Press 1991
Academic Press
eBook ISBN:
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About the Serial Volume Editors

Richard Vallee Serial Volume Editor

Affiliations and Expertise

Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts, U.S.A.

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA