Molecular Microbial Diagnostic Methods - 1st Edition - ISBN: 9780124169999, 9780124171701

Molecular Microbial Diagnostic Methods

1st Edition

Pathways to Implementation for the Food and Water Industries

Editors: Nigel Cook Martin D'Agostino K. Clive Thompson
eBook ISBN: 9780124171701
Hardcover ISBN: 9780124169999
Imprint: Academic Press
Published Date: 1st June 2015
Page Count: 262
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Molecular Microbial Diagnostic Methods: Pathways to Implementation for the Food and Water Industry was developed by recognized and experienced highlevel scientists. It’s a comprehensive and detailed reference that uncovers industry needs for the use of molecular methods by providing a brief history of water and food analysis for the pathogens of concern. It also describes the potential impact of current and cutting-edge molecular methods. This book discusses the advantages of the implementation of molecular methods, describes information on when and how to use specific methods, and presents why one should utilize them for pathogen detection in the routine laboratory. The content is also pertinent for anyone carrying out microbiological analysis at the research level, and for scientists developing methods, as it focuses on the requirements of end-users.

Key Features

  • Includes information on how to introduce and implement molecular methods for routine monitoring in food and water laboratories
  • Discusses the importance of robust validation of molecular methods as alternatives to existing standard methods to help ensure the production of defendable results
  • Highlights potential issues with respect to successful implementation of these methods


Laboratory managers; senior microbiologists in routine food and water microbiological laboratories

Table of Contents

  • Contributors
  • Preface
  • Chapter 1: Food industry current status
    • Abstract
    • Introduction
    • Molecular methods currently used
    • Laboratory automation in molecular methods
    • Challenges
    • Acceptance of use by the food industry
    • Compliance with current legal requirements
    • Disclaimer
  • Chapter 2: Future directions for molecular microbial diagnostic methods for the food industry
    • Abstract
    • Introduction
    • Evolution of food microbiology diagnostics: from petri dishes to PCR
    • Why introduce an alternative molecular diagnostic method?
    • Characteristics of an ideal food molecular method
    • Past and current challenges
    • Current challenges
    • Concluding remarks
  • Chapter 3: Current status of molecular microbiological techniques for the analysis of drinking water
    • Abstract
    • Introduction and overview
    • The current state of play
    • The influence of standard and reference methods
    • Molecular techniques for testing potable water quality
    • Application of molecular techniques to wastewater
    • Conclusions
  • Chapter 4: What is now required for water?
    • Abstract
    • Introduction and overview
    • Indicator organisms
    • Pathogens in drinking water
    • Total heterotrophic bacteria
    • Feasibility of the use of routine rapid water molecular methods
    • Future predictions
    • Conclusions
  • Chapter 5: CEN/ISO standards for both culture and molecular methods
    • Abstract
    • Introduction
    • Standards, standardization bodies, and structures in the microbiology of the food chain
    • Standards developed in the microbiology of the food chain
    • Status of novel technologies
    • Conclusions
  • Chapter 6: Laboratory validation, verification, and accreditation of molecular methods
    • Abstract
    • Alternative methods
    • Foundations of an accredited laboratory
    • Quality systems
    • Cornerstones of accreditation
    • Maintaining accreditation
    • Customer education
    • Health and safety
  • Chapter 7: DNA extraction: finding the most suitable method
    • Abstract
    • Boiling method
    • Column extraction
    • Magnetic beads
    • FTA™ cards
    • RNA extraction methods
    • Conclusions
  • Chapter 8: Assessing organism viability and interpreting genomic unit versus colony forming unit data for water and food borne microorganisms, such as Legionella, Campylobacter, Salmonella, and Listeria
    • Abstract
    • Introduction
    • Polymerase chain reaction
    • Assessing microbial viability
    • Overcoming the viability hurdle
    • When to use viability discrimination
    • Standardization and quality assurance
    • Proficiency testing
    • Conclusions
  • Chapter 9: MALDI-TOF MS: a rapid microbiological confirmation technique for food and water analysis
    • Abstract
    • Introduction and overview
    • Current confirmation methods and advantages of MALDI-TOF MS
    • MALDI-TOF MS Listeria speciation validation
  • Chapter 10: Chapter highlights, future requirements, and conclusions
    • Chapter highlights
    • Future requirements and conclusions
  • Index


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© Academic Press 2016
Academic Press
eBook ISBN:
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About the Editor

Nigel Cook

Dr. Nigel Cook is Senior microbiologist at The Food and Environment Research Agency (FERA) in the UK. He specializes in integrated monitoring and control of foodborne viruses in European Food Supply Chains.

Affiliations and Expertise

Research Scientist, Fera Science Ltd., York, United Kingdom

Martin D'Agostino

Martin D’Agostino has worked for Fera Science Ltd, formerly the Food and Environment Research Agency (Fera) since 1994. His microbiological career began with Kennerty Farm Dairies in Aberdeen (now Robert Wisemans) as a laboratory assistant, until he joined what was the Torry Food Science Laboratory (Central Science Laboratory, MAFF) in 1994, now named the Food and Environment Research Agency as part of Defra. He has been involved in EU Framework 5, 6 and 7 projects dealing with such areas as Food PCR, Surveillance of Adenovirus and Norovirus in European Bathing waters and more recently has assisted in the coordination of the FP7 project entitled “Integrated monitoring and Control of Foodborne Viruses in European Food Supply Chains” ( This has resulted in the accumulation of many European and worldwide contacts from both Academia and Industry related to both food and environmental microbiology. Martin has experience of chairing and presenting scientific talks at international scientific meetings and has been an author in at least 25 peer reviewed articles including journal manuscripts, several reviews and book chapters and is an editor of a book, related to the detection and implementation of rapid methods for foodborne and environmental human pathogens.

Affiliations and Expertise

Microbiologist, Fera Science Ltd., York, United Kingdom

K. Clive Thompson

Dr. K. Clive Thompson is Fellow of the Royal Society of Chemistry; Fellow of the Institute of Food Science & Technology; Fellow of the Royal Society of Public Health; Member of the Water Management Society; Member of the American Chemical Society; Member of the American Society for Microbiology; Fellow of the Chartered Institute of Water and Environmental Management; Member of Board of Trustees of Society of Chemical Industry; Society of Chemical Industry (SCI) Environmental Medal (2003); Member of AOAC; Member of the Society of Environmental Toxicology and Chemistry; Distinguished Service Certificate, British Standards in appreciation of long and valued contributions to the development of British, European and International Standards; and Visiting Professor at Brunel University.

He is currently Chief Scientist at ALcontrol Laboratories UK, which analyses a very wide range of samples including food, drinking water, process waters, and effluents for both chemical and microbiological parameters. It has eight laboratories in the UK and is one of the largest contract contaminated land analysis / water analysis / food analysis laboratory organisations in Europe. Dr Thompson previously worked for Yorkshire water and Severn Trent Water. He is a member of many national and international standardisation committees and has published more than 40 peer-reviewed papers and co-edited a significant number of books.

Affiliations and Expertise

Chief Scientist, ALcontrol Laboratories, South Yorkshire, United Kingdom

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